Strain number NIES-458  
Phylum Chlorophyta  
Class Chlorophyceae  
Scientific name Eudorina elegans Ehrenberg var. synoica Goldstein  
Synonym  
Former name  
Common name Green alga  
Locality (Date of collection) Midori-ku, Yokohama Kanagawa Japan (1980-01-**)  
Latitude / Longitude 35.515457 / 139.526291 
Habitat (Isolation source) Freshwater (Paddy soil)  
History < Nozaki, Hisayoshi  
Isolator (Date of isolation) Nozaki, Hisayoshi (1980-04-27)  
Identified by Nozaki, Hisayoshi  
State of strain Subculture; Unialgal; Clonal; Axenic[2017 Dec]  
Culture condition
(Preculture condition)
Medium:  VT  
Temperature:  20 C
Light intensity:  8 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  1 M  
Gene information rbcL ( D88807 )  
Cell size (min - max) - 35 μm  
Organization Colonial; Flagellate 
Characteristics Phototaxis ; Homothallic ; Monoecious  
Other strain no. Other strain no. : 04427-1  
Remarks Axenic 
Movie  
Reference
Hanschen, E. R., Herron, M. D., Wiens, J. J., Nozaki, H., Michod, R. E. 2018 Repeated evolution and reversibilityof self-fertilization in the volvocinegreen algae. Evolution, 72, 386-398.
Keywords: Haploid; phylogenetics; self-fertilization; sex; sexual reproduction; volvocine green algae
Strain(s): 28941845645846054154354454556456656856957157257457858265373285185685787287587687787888088288389189210381362171117132457256927383382338837803782 
PubMed: 29134623
DOI: 10.1111/evo.13394

Hanschen, E. R., Herron, M. D., Wiens, J. J., Nozaki, H., Michod, R. E. 2018 Multicellularity drives the evolution of sexual traits. Am. Nat., 192, E93-E10.
Keywords: sex; volvocine green algae; multicellularity; ancestral state; reconstruction; anisogamy; sexual dimorphism
Strain(s): 28941842145645846052254154354454556456656856957157257457858265373275185185685787287587687787888088288389189210381362171117132457256927383382338837803782 
DOI: 10.1086/698301

Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55.
Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection
Strain(s): 19212223242526272829303132333435363738394041444546505152535455565758596061626364656667686970737475767778798081878990919293949596979899100101102103104105106107108109110111112119120122123124125127128129130131132133134135137138139144147148150151152153154155156157158159160162163164165166167168169170171172173174175176177178179180181182183185186187188189191192193194195197198199200201202203204205206207208209210211212213214215216217224226227228229230231232241242243244245246248257259261262263266267268271287288289290294295297298299300301302303305306308309310311312313329334336337338339340341342349351359360361362375378379380382384385390394396397398415416418419421422423424425426427428429430431432433434436437438439440446447448449450451452453454455456457458459460464468469474478479480481503504505506507509510512514515522523524527528529530531532536537538539540541542543544545546564565566567568569570571572573574575576577578579580581582583584585592593594595596597598604610628630631632633634635636637638639640641642643644645646647648649650651652653654655656657658659660661662663664665666667672685686687688689690691692693694717718719720721722723724725726727728729 

Nozaki, H., Ito, M., Uchida, H., Watanabe, M. M., Kuroiwa, T. 1997 Phylogenetic analysis of Eudorina species (Volvocaceae, Chlorophyta) based on rbcL gene sequences. J. Phycol., 33, 859-863.
Keywords: Chlorophyta; Eudorina; large subunit of ribulose-1,5-bisphosphate carboxylase / oxygenase (rbcL) gene; molecular phylogeny; species delimitation; taxonomic species; Volvocaceae
Strain(s): 458568717718719720721723 
DOI: 10.1111/j.0022-3646.1997.00859.x

Nozaki, H. 1986 Zygote germination in Eudorina elegans var. synoica (Chlorophyta, Volvocales). J. Jpn. Bot., 61, 316-320, pl. 8.
Strain(s): 458 

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