The Microbial Culture Collection at the National Institute for Environmental Studies

Strain Data


Strain number NIES-30  
Phylum Cyanophyta   Class Cyanophyceae 
Scientific name Leptolyngbya sp.  
Synonym  
Former name Phormidium tenue (C.Agardh ex Gomont) Anagnostidis et Komárek  
Common name Blue-green alga  
Locality (Date of collection) Akita Akita Japan (-----)  
Habitat (Isolation source) Freshwater (Paddy field)  
History < IAM (1983);  
Isolator (Date of isolation) Ishikawa, Masako (-----)  
Identified by Fukushima, Hiroshi; Watanabe, Makoto M. (Reidentify); Homma, Takamitsu (Re-reidentify)  
State of strain Unialgal; Non-clonal; Non-axenic  
Preservation state
(State of preservation)
Cryopreservation  
Culture condition
( Preculture condition )
Medium:  MDM (agar)  
Temperature: 20(25) C
Light intensity: 4-10(70-80) µmol photons/m2/sec     L/D cycle: 12L:12D
Duration: 4 M(14 D)  
Gene information 16S rRNA ( AB042857 )
Cell size (min - max) 1 - 3 μm   Organization Filamentous 
Characteristics  
Other strain no. Other collection strain no. : IAM M-40
 
Remarks Cryopreserved 

Reference
236)   Hodoki, Y., Ohbayashi, K., Kobayashi, Y., Okuda, N., Nakano, S. 2012 Detection and identification of potentially toxic cyanobacteria: ubiquitous distribution of Microcystis aeruginosa and Cuspidothrix issatschenkoi in Japanese lakes. Harmful Algae, 16, 49-57. , Keywords: Anatoxin-a; Automated rRNA intergenic spacer analysis (ARISA); Cuspidothrix issatschenkoi; Cyanotoxin; Microcystin; Microcystis aeruginosa , Strain(s): 304077102111230512843905928932937991164516691698172919502116 

270)   Ichimura, T., Itoh, T. 1977 17. Preservation methods of microalgae (I) [17. Bisaisôrui no hozonhô (I)]. In Preservation methods of microorganisms [Biseibutsu Hozonhô], Ed. by Nei, T., University of Tokyo Press, Tokyo, p. 355-373 (in Japanese without English title). , Strain(s): 19212223242728303132333436373839404144454647484950122123127131144152173175179180181182183185198200203206217226227241266267268271295309310334338379436448450455527 

632)   Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55. , Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection , Strain(s): 19212223242526272829303132333435363738394041444546505152535455565758596061626364656667686970737475767778798081878990919293949596979899100101102103104105106107108109110111112119120122123124125127128129130131132133134135137138139144147148150151152153154155156157158159160162163164165166167168169170171172173174175176177178179180181182183185186187188189191192193194195196197198199200201202203204205206207208209210211212213214215216217224226227228229230231232241242243244245246248257259261262263266267268271287288289290294295297298299300301302303305306308309310311312313329334336337338339340341342349351359360361362375378379380382384385390394396397398415416418419421422423424425426427428429430431432433434436437438439440446447448449450451452453454455456457458459460464468469474478479480481503504505506507509510512514515522523524527528529530531532536537538539540541542543544545546564565566567568569570571572573574575576577578579580581582583584585592593594595596597598604610628630631632633634635636637638639640641642643644645646647648649650651652653654655656657658659660661662663664665666667672685686687688689690691692693694717718719720721722723724725726727728729 

869)   Oikawa, E., Ishibashi, Y., Abe, T., Umetsu, H. 2000 Phylogenetic classification of musty odor and/or toxic compounds producing cyanobacteria. Environ. Eng. Res., 37, 183-191 (in Japanese with English summary). , Keywords: cyanobacteria; musty odor; 16S ribosomal RNA; PCR; phylogenetic trees , Strain(s): 213033417376512 

1055)   Shinobu, K., Abe, S., Asano, K., Abe, T. 1992 On the laboratory experiment of algal culturing (AGP-test) [Sôrui baiyô no shitsunai jikken (AGP shiken) ni tsuite]. Heisei 4-nendo Tôhoku Chihô Kensetsu-kyoku Kan-nai Gijutsu Kenkyû Happyôkai Shiryô, Kyô-28, 151-154 (in Japanese without English title). , Strain(s): 30