Strain number NIES-2564  
Phylum Chlorophyta  
Class Chlorophyceae  
Scientific name Pyrobotrys squarrosa (Korshikov) Korshikov  
Synonym Chlamydobotrys squarrosa Korshikov 1928; Uva squarrosa (Korshikov) Fott 1967; Pyrobotrys squarrosa (Korshikov) Huber-Pestalozzi in Fott 1967  
Former name  
Common name Green alga  
Locality (Date of collection) Otsuka-minami, Toyama Toyama Japan (2005-09-23)  
Latitude / Longitude 36.72722222 / 137.16333333 
Habitat (Isolation source) Freshwater (Paddy soil)  
History < Nakada, Takashi  
Isolator (Date of isolation) Nakada, Takashi (2009-07-16)  
Identified by Nakada, Takashi  
State of strain Subculture; Unialgal; Clonal; Axenic[2017 Dec]  
Culture condition
(Preculture condition)
Medium:  mAC  
Temperature:  20 C
Light intensity:  7-13 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  1 M  
Gene information 18S rRNA ( AB542919 ) , atpB ( LC066350 ) , psaB ( AB542924 ) , rbcL ( AB542927 )  
Cell size (min - max) 8 - 18 μm  
Organization  
Characteristics Mixotrophic ; Osmotrophic ; Resting spore-forming  
Other strain no. Other collection strain no. : NBRC 107039
Other strain no. : KrCl905  
Remarks Fragile species to transportation stresses; Read and agree "How to order 4.1"; Axenic 
Movie  
Reference
Munakata, H., Nakada, T., Nakahigashi, K., Nozaki, H., Tomita, M. 2016 Phylogenetic position and molecular chronology of a colonial green flagellate, Stephanosphaera pluvialis (Volvocales, Chlorophyceae), among unicellular algae. J. Eucaryot. Microbiol., 63, 340-348.
Keywords: Balticola; coloniality; divergence time; evolution; molecular phylogeny
Strain(s): 12314442142843243944656756957165686188796810482293256441244125 
PubMed: 26595722
DOI: 10.1111/jeu.12283

Nakada, T., Nozaki, H., Tomita, M. 2010 Another origin of coloniality in volvocaleans: the phylogenetic position of Pyrobotrys Arnoldi (Spondylomoraceae, Volvocales). J. Eucaryot. Microbiol., 57, 379-382.
Keywords: Chlorophyceae; molecular phylogeny; multicellularity
Strain(s): 2564 
PubMed: 20553352
DOI: 10.1111/j.1550-7408.2010.00488.x

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