The Microbial Culture Collection at the National Institute for Environmental Studies

Strain Data


Strain number NIES-418  
Phylum Chlorophyta   Class Chlorophyceae 
Scientific name Astrephomene gubernaculifera Pocock  
Synonym -----  
Former name  
Common name Green alga  
Locality (Date of collection) Kanagawa Japan (1981-04-**)  
Habitat (Isolation source) Freshwater (Paddy soil)  
History < Nozaki, Hisayoshi  
Isolator (Date of isolation) Nozaki, Hisayoshi (1981-05-20)  
Identified by Nozaki, Hisayoshi  
State of strain Unialgal; Clonal; Axenic[2012 Dec]  
Preservation state
(State of preservation)
Subculture  
Culture condition
( Preculture condition )
Medium:  VTAC  
Temperature: 20 C
Light intensity: 15-22 µmol photons/m2/sec     L/D cycle: 12L:12D
Duration: 20 D  
Gene information atpB ( AB014022 AB014023 ) , psaA ( AB044233 AB044234 ) , psaB ( AB044458 ) , psbC ( AB044513 AB044514 ) , rbcL ( D63428 ) , rbcL-462 intron ( AB076095 )
Cell size (min - max) - 35 μm   Organization Colonial; Flagellate 
Characteristics Heterothallic; Mating type(−); Crosses with NIES-419  
Other strain no. Other strain no. : 1520-4 (−)  
Remarks  

Reference
635)   Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55. , Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection , Strain(s): 19212223242526272829303132333435363738394041444546505152535455565758596061626364656667686970737475767778798081878990919293949596979899100101102103104105106107108109110111112119120122123124125127128129130131132133134135137138139144147148150151152153154155156157158159160162163164165166167168169170171172173174175176177178179180181182183185186187188189191192193194195196197198199200201202203204205206207208209210211212213214215216217224226227228229230231232241242243244245246248257259261262263266267268271287288289290294295297298299300301302303305306308309310311312313329334336337338339340341342349351359360361362375378379380382384385390394396397398415416418419421422423424425426427428429430431432433434436437438439440446447448449450451452453454455456457458459460464468469474478479480481503504505506507509510512514515522523524527528529530531532536537538539540541542543544545546564565566567568569570571572573574575576577578579580581582583584585592593594595596597598604610628630631632633634635636637638639640641642643644645646647648649650651652653654655656657658659660661662663664665666667672685686687688689690691692693694717718719720721722723724725726727728729 

734)   Nakazawa, A., Nishii, I. 2012 Amidic and acetonic cryoprotectants improve cryopreservation of volvocine green algae. CryoLetters, 33, 201-212. , Keywords: cryopreservation; dimethylformamide; hydroxyacetone; Volvocaceae; Volvox; Chlamydomonas , Strain(s): 418468545564574717721728729730731877133113611710 

764)   Nozaki, H. 1983 Morphology and taxonomy of two species of Astrephomene (Chlorophyta, Volvocales) in Japan. J. Jpn. Bot., 58, 345-352, pls. 11-13. , Strain(s): 418419564565 

789)   Nozaki, H., Ito, M., Sano, R., Uchida, H., Watanabe, M. M., Kuroiwa, T. 1995 Phylogenetic relationships within the colonial Volvocales (Chlorophyta) inferred from rbcL gene sequence data. J. Phycol., 31, 970-979. , Keywords: Chlorophyta; Goniaceae; large subunit of ribulose-1,5-bisphosphate carboxylase / oxygenase (rbcL) gene; molecular phylogeny; Tetrabaenaceae; Volvocaceae , Strain(s): 418421456460541564566569571572574577653725731732734735737895 

807)   Nozaki, H., Misawa, K., Kajita, T., Kato, M., Nohara, S., Watanabe, M. M. 2000 Origin and evolution of the colonial Volvocales (Chlorophyceae) as inferred from multiple, chloroplast gene sequences. Mol. Phylog. Evol., 17, 256-268. , Strain(s): 418456460474541545564566569571572574577582653654666720721722724725727728730731732734735736737851854872882884886887888889890893895896898 

812)   Nozaki, H., Ohta, N., Takano, H., Watanabe, M. M. 1999 Reexamination of phylogenetic relationships within the colonial Volvocales (Chlorophyta): an analysis of atpB and rbcL gene sequences. J. Phycol., 35, 104-112. , Keywords: atpB gene; Chlorophyta; Gonium; molecular phylogeny; rbcL gene; Volvocaceae; volvocine series; Volvox , Strain(s): 418456460541564566568569571572574577582653654666720721722724725727728730731732734735736737851852858861882884895 

814)   Nozaki, H., Onishi, K., Morita, E. 2002 Differences in pyrenoid morphology are correlated with differences in the rbcL genes of members of the Chloromonas lineage (Volvocales, Chlorophyceae). J. Mol. Evol., 55, 414-430. , Keywords: ATP synthase β-subunit (atpB) gene; Chloromonas; gene evolution; large subunit of Rubisco (rbcL) gene; morphological evolution; P700 chlorophyll a-apoprotein A2 (psaB) gene; pyrenoid; Volvocales , Strain(s): 96123144213418421425428432439446447456474522564566567569571574582653732851858861875882883968 

817)   Nozaki, H., Takahara, M., Nakazawa, A., Kita, Y., Yamada, T., Takano, H., Kawano, S., Kato, M. 2002 Evolution of rbcL group IA introns and intron open reading frames within the colonial Volvocales (Chlorophyceae). Mol. Phylog. Evol., 23, 326-338. , Strain(s): 289418582654857875883891892