Strain number NIES-213  
Phylum Chlorophyta  
Class Chlorophyceae  
Scientific name Pediastrum duplex Meyen var. duplex  
Synonym Pediastrum napoleonis Ralfs; Pediastrum pertusum Kützing; Pediastrum duplex f. genuinum Arnold & Aleksenko; Pediastrum selenaea Kützing 1845; Pediastrum pertusum var. genuinum A.Braun 1855; Pediastrum duplex var. reticulatum Lagerheim 1882; Pediastrum duplex var. clathratum (A.Braun) Lagerheim 1882; Pediastrum duplex var. genuinum (A.Braun) Lagerheim 1882  
Former name  
Common name Green alga  
Locality (Date of collection) Tsukuba Ibaraki Japan (1983-05-25)  
Latitude / Longitude 36.105319 / 140.081738 
Habitat (Isolation source) Freshwater (Pond water)  
History < Hiwatari, Takehiko  
Isolator (Date of isolation) Hiwatari, Takehiko (1983-06-14)  
Identified by Hiwatari, Takehiko; Watanabe, Masayuki (Reidentify)  
State of strain Cryopreservation; Unialgal; Clonal; Axenic  
Culture condition
(Preculture condition)
Medium:  C  
Temperature:  20 C
Light intensity:  8-15 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  2 M  
Gene information atpB ( AB084306 ) , rbcL ( AB084333 ) , psaB ( AB084340 ) , Pcy ( AB218890 )  
Cell size (min - max)  
Organization Colonial 
Characteristics  
Other strain no. Other strain no. : AQ-P-1  
Remarks Cryopreserved; Axenic 
Movie  
Reference
Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55.
Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection
Strain(s): 19212223242526272829303132333435363738394041444546505152535455565758596061626364656667686970737475767778798081878990919293949596979899100101102103104105106107108109110111112119120122123124125127128129130131132133134135137138139144147148150151152153154155156157158159160162163164165166167168169170171172173174175176177178179180181182183185186187188189191192193194195197198199200201202203204205206207208209210211212213214215216217224226227228229230231232241242243244245246248257259261262263266267268271287288289290294295297298299300301302303305306308309310311312313329334336337338339340341342349351359360361362375378379380382384385390394396397398415416418419421422423424425426427428429430431432433434436437438439440446447448449450451452453454455456457458459460464468469474478479480481503504505506507509510512514515522523524527528529530531532536537538539540541542543544545546564565566567568569570571572573574575576577578579580581582583584585592593594595596597598604610628630631632633634635636637638639640641642643644645646647648649650651652653654655656657658659660661662663664665666667672685686687688689690691692693694717718719720721722723724725726727728729 

Nozaki, H., Onishi, K., Morita, E. 2002 Differences in pyrenoid morphology are correlated with differences in the rbcL genes of members of the Chloromonas lineage (Volvocales, Chlorophyceae). J. Mol. Evol., 55, 414-430.
Keywords: ATP synthase β-subunit (atpB) gene; Chloromonas; gene evolution; large subunit of Rubisco (rbcL) gene; morphological evolution; P700 chlorophyll a-apoprotein A2 (psaB) gene; pyrenoid; Volvocales
Strain(s): 96123144213418421425428432439446447456474522564566567569571574582653732851858861875882883968 
PubMed: 12355262
DOI: 10.1007/s00239-002-2338-9

Hiwatari, T., Kasai, F., Watanabe, M. M., Nei, T. 1984 Cryopreservation of Scenedesmus acutus and Pediastrum duplex in liquid nitrogen - Survival and growth after freezing. Jpn. J. Freez. Dry., 30, 27-31 (in Japanese with English title).
Strain(s): 95213 

Watanabe, M. M., Kasai, F., Hiwatari, T., Suda, S., Nei, T. 1984 Cryopreservation of microalgae in liquid nitrogen - Survival of algal cultures after freezing. Jpn. J. Freez. Dry., 30, 23-26 (in Japanese with English title).
Strain(s): 1819212426293139404144454650737475767780818788899091939598991001011201221311351371441512042072132572633794152219223522392579 

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