Strain number NIES-2541  
Phylum Chlorophyta  
Class Trebouxiophyceae  
Scientific name Chlorella variabilis Shihira & Krauss  
Synonym Chlorella paramecii Loefer 1936  
Former name  
Common name Green alga  
Locality (Date of collection) Aichi Japan  
Latitude / Longitude  
Habitat (Isolation source) (Paramecium bursaria OK1)  
History < Hoshina, Ryo  
Isolator (Date of isolation) Kamako, Shin-ichiro (2003-**-**)  
Identified by Hoshina, Ryo  
State of strain Cryopreservation; Unialgal; Clonal; Axenic[2018 Mar]  
Culture condition
(Preculture condition)
Medium:  CYTHUT  
Temperature:  20 C
Light intensity:  4-10 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  1 M  
Gene information rbcL ( AB260899 ) , 28S rRNA ( AB437257 ) , psaA ( AB260913 ) , 18S-ITS1-5.8S-ITS2 ( AB162912 ) , 16S rRNA ( AB208587 )  
Cell size (min - max) 3 - 7 μm  
Organization Unicellular 
Characteristics Symbiotic (Paramecium bursaria) ; Auxotrophic (Amino acid)  
Other strain no. Other strain no. : OK1-ZK  
Remarks Cryopreserved; Axenic 
Movie  
Reference
Yokono, M., Takabayashi, A., Kishimoto, J., Fujita, T., Iwai, M., Murakami, A., Akimoto, S., Tanaka, A. 2019 The PSI-PSII megacomplex in green plants. Plant Cell Physiol., ,
Strain(s): 14429630322852541 
PubMed: 30753722
DOI: 10.1093/pcp/pcz026

Hamada, M., Schröder, K., Bathia, J., Kürn, U., Fraune, S., Khalturina, M., Khalturin, K., Shinzato, C., Satoh, N., Bosch, T. C.G. 2018 Metabolic co-dependence drives the evolutionary ancient Hydra-Chlorella symbiosis. eLife, e35122 (article ID).
Keywords: Chlorella; Hydra; evolutionary biology; genome; nitrogen metabolism; symbiosis
Strain(s): 216622352541 
PubMed: 29848439
DOI: 10.7554/eLife.35122

Quispe, C. F., Sonderman, O., Khasin, M., Riekhof, W. R., Van Etten, J. L., Nickerson, K. W. 2016 Comparative genomics, transcriptomics, and physiology distinguish symbiotic from free-living Chlorella strains. Algal Res., 18, 332-340.
Keywords: Chlorella variabilis; Alga-paramecium symbiosis; Algal nitrogen metabolism; Amino acid transporters
Strain(s): 25402541 
DOI: 10.1016/j.algal.2016.06.001

Kunugi, M., Satoh. S., Ihara. K., Shibata. K., Yamagishi. Y., Kogame. K., Obokata. J., Takabayashi. A., Tanaka. A. 2016 Evolution of green plants accompanied changes in light-harvesting systems. Plant Cell Physiol., 57, 1231-1243.
Keywords: Evolution; Green algae; Land plants; Light adaptation; Photosystem
Strain(s): 254141925412582 
PubMed: 27057002
DOI: 10.1093/pcp/pcw071

Hoshina, R., Imamura, N. 2009 Phylogenetically close group I introns with different positions among Paramecium bursaria photobionts imply a primitive stage of intron diversification. Mol. Biol. Evol., 26, 1309-1319.
Keywords: degenerative homing; intron diversification; intron transposition; Paramecium bursaria symbiotic algae; reverse splicing
Strain(s): 2541 
PubMed: 19279084
DOI: 10.1093/molbev/msp044

Kato, Y., Ueno, S., Imamura, N. 2006 Studies on the nitrogen utilization of endosymbiotic algae isolated from Japanese Paramecium bursaria. Plant Sci., 170, 481-486.
Keywords: Endosymbiont; Paramecium bursaria; Nitrogen utilization; Chlorella
Strain(s): 22725402541 
DOI: 10.1016/j.plantsci.2005.09.018

Hoshina, R., Kato, Y., Kamako, S., Imamura, N. 2005 Genetic evidence of "American" and "European" type symbiotic algae of Paramecium bursaria Ehrenberg. Plant Biol., 7, 526-532.
Keywords: 18S rDNA; "American"; "European"; Chlorella; ITS; Paramecium bursaria; Phylogeny
Strain(s): 25402541 
DOI: 10.1055/s-2005-865912

Hoshina, R., Kamako, S.-I., Imamura, N. 2004 Phylogenetic position of endosymbiotic green algae in Paramecium bursaria Ehrenberg from Japan. Plant Biol., 6, 447-453.
Keywords: 18S rDNA; Chlorella; endosymbiont; ITS; Paramecium bursaria; phylogeny
Strain(s): 25402541 
DOI: 10.1055/s-2004-820888

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