Strain number NIES-2540  
Phylum Chlorophyta  
Class Trebouxiophyceae  
Scientific name Chlorella variabilis Shihira & Krauss  
Synonym Chlorella paramecii Loefer 1936  
Former name  
Common name Green alga  
Locality (Date of collection) Japan  
Latitude / Longitude  
Habitat (Isolation source) (Paramecium bursaria F36)  
History < Hoshina, Ryo  
Isolator (Date of isolation) Kamako, Shin-ichiro (2002-**-**)  
Identified by Hoshina, Ryo  
State of strain Cryopreservation; Unialgal; Clonal; Axenic[2018 Mar]  
Culture condition
(Preculture condition)
Medium:  CYTHUT  
Temperature:  20 C
Light intensity:  4-10 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  1 M  
Gene information 16S rRNA ( AB208588 ) , 18S-ITS1-5.8S-ITS2 ( AB162914 ) , rbcL ( AB260900 ) , psaA ( AB260914 )  
Cell size (min - max) 5 - 8 μm  
Organization Unicellular 
Characteristics Symbiotic (Paramecium bursaria) ; Auxotrophic (Amino acid)  
Other strain no. Other strain no. : F36-ZK  
Remarks Cryopreserved; Axenic 
Movie  
Reference
Hamana, K., Kobayashi, M., Furuchi, T., Hayashi, H., Niitsu, M. 2018 Polyamine analysis of unicellular, colonial, and multicellular green algae —Detection of aminobutylcadaverine, N1-aminopentylspermidine, N8-aminopentylspermidine, and penta-amines.— Microb. Resour. Syst., 34, 73-82.
Keywords: aminobutylcadaverine; aminopentylspermidine; green alga; penta-amine; polyamine; thermospermine
Strain(s): 1835242691735127112782185218722352261254025723983 

Quispe, C. F., Sonderman, O., Khasin, M., Riekhof, W. R., Van Etten, J. L., Nickerson, K. W. 2016 Comparative genomics, transcriptomics, and physiology distinguish symbiotic from free-living Chlorella strains. Algal Res., 18, 332-340.
Keywords: Chlorella variabilis; Alga-paramecium symbiosis; Algal nitrogen metabolism; Amino acid transporters
Strain(s): 25402541 
DOI: 10.1016/j.algal.2016.06.001

Hoshina, R., Iwataki, M., Imamura, N. 2010 Chlorella variabilis and Micractinium reisseri sp. nov. (Chlorellaceae, Trebouxiophyceae): Redescription of the endosymbiotic green algae of Paramecium bursaria (Peniculia, Oligohymenophorea) in the 120th year. Phycol. Res., 58, 188-201.
Keywords: Chlorella variabilis; ITS2; Micractinium reisseri; Paramecium bursaria; species concept
Strain(s): 25402542 
DOI: 10.1111/j.1440-1835.2010.00579.x

Kato, Y., Imamura, N., 2008 Effect of calcium ion on uptake of amino acids by symbiotic Chlorella F36-ZK isolated from Japanese Paramecium bursaria. Plant Sci., 174, 88-96.
Keywords: Paramecium bursaria; Chlorella; Calcium; Divalent cation; Amino acid transport; Symbiosis
Strain(s): 22721572540 
DOI: 10.1016/j.plantsci.2007.10.001

Kato, Y., Ueno, S., Imamura, N. 2006 Studies on the nitrogen utilization of endosymbiotic algae isolated from Japanese Paramecium bursaria. Plant Sci., 170, 481-486.
Keywords: Endosymbiont; Paramecium bursaria; Nitrogen utilization; Chlorella
Strain(s): 22725402541 
DOI: 10.1016/j.plantsci.2005.09.018

Hoshina, R., Kato, Y., Kamako, S., Imamura, N. 2005 Genetic evidence of "American" and "European" type symbiotic algae of Paramecium bursaria Ehrenberg. Plant Biol., 7, 526-532.
Keywords: 18S rDNA; "American"; "European"; Chlorella; ITS; Paramecium bursaria; Phylogeny
Strain(s): 25402541 
DOI: 10.1055/s-2005-865912

Hoshina, R., Kamako, S.-I., Imamura, N. 2004 Phylogenetic position of endosymbiotic green algae in Paramecium bursaria Ehrenberg from Japan. Plant Biol., 6, 447-453.
Keywords: 18S rDNA; Chlorella; endosymbiont; ITS; Paramecium bursaria; phylogeny
Strain(s): 25402541 
DOI: 10.1055/s-2004-820888

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