The Microbial Culture Collection at the National Institute for Environmental Studies

Strain Data


Strain number NIES-447  
Phylum Chlorophyta   Class Chlorophyceae 
Scientific name Chloromonas insignis (Anachin) Gerloff et Ettl  
Synonym -----  
Former name  
Common name Green alga  
Locality (Date of collection) Lake Kasumigaura Tsuchiura Ibaraki Japan (1983-08-22)  
Habitat (Isolation source) Freshwater (Lake wate)  
History < Suda, Shoichiro  
Isolator (Date of isolation) Suda, Shoichiro (1983-08-24)  
Identified by Suda, Shoichiro  
State of strain Unialgal; Clonal; Axenic[2013 Feb]  
Preservation state
(State of preservation)
Subculture  
Culture condition
( Preculture condition )
Medium:  C  
Temperature: 20 C
Light intensity: 22-32 µmol photons/m2/sec     L/D cycle: 12L:12D
Duration: 2 M  
Gene information 18S rRNA ( AB504776 AB624567 ) , atpB ( AB084313 ) , psaA ( AB624593 ) , psaB ( AB084348 ) , rbcL ( AB022226 ) , ITS2 ( AB624578 )
Cell size (min - max)   Organization Unicellular; Flagellate 
Characteristics  
Other strain no. Other strain no. : Kas-8  
Remarks  

Reference
589)   Matsuzaki, R., Hara, Y., Nozaki, H. 2012 A taxonomic revision of Chloromonas reticulata (Volvocales, Chlorophyceae), the type species of the genus Chloromonas, based on multigene phylogeny and comparative light and electron microscopy. Phycologia, 51, 74-85. , Keywords: Chloromonadinia; Chloromonas; Chloromonas chlorococcoides comb. nov.; Chloromonas lineage; Chloromonas typhlos comb. nov.; Molecular phylogeny; Morphology; Taxonomy; Ultrastructure; Volvocales , Strain(s): 447 

653)   Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55. , Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection , Strain(s): 19212223242526272829303132333435363738394041444546505152535455565758596061626364656667686970737475767778798081878990919293949596979899100101102103104105106107108109110111112119120122123124125127128129130131132133134135137138139144147148150151152153154155156157158159160162163164165166167168169170171172173174175176177178179180181182183185186187188189191192193194195196197198199200201202203204205206207208209210211212213214215216217224226227228229230231232241242243244245246248257259261262263266267268271287288289290294295297298299300301302303305306308309310311312313329334336337338339340341342349351359360361362375378379380382384385390394396397398415416418419421422423424425426427428429430431432433434436437438439440446447448449450451452453454455456457458459460464468469474478479480481503504505506507509510512514515522523524527528529530531532536537538539540541542543544545546564565566567568569570571572573574575576577578579580581582583584585592593594595596597598604610628630631632633634635636637638639640641642643644645646647648649650651652653654655656657658659660661662663664665666667672685686687688689690691692693694717718719720721722723724725726727728729 

658)   Morita, E., Abe, T., Tsuzuki, M., Fujiwara, S., Sato, N., Hirata, A., Sonoike, K., Nozaki, H. 1999 Role of pyrenoids in the CO2-concentrating mechanism: comparative morphology, physiology and molecular phylogenetic analysis of closely related strains of Chlamydomonas and Chloromonas (Volvocales). Planta, 208, 365-372. , Keywords: Chlamydomonas; Chloromonas; CO2-concentrating mechanism; molecular phylogeny; pyrenoid; ribulose-1,5-bisphosphate carboxylase/oxygenase , Strain(s): 447 

832)   Nozaki, H., Nakada, T., Watanabe, S. 2010 Evolutionary origin of Gloeomonas (Volvocales, Chlorophyceae), based on ultrastructure of chloroplasts and molecular phylogeny. J. Phycol., 46, 195-201. , Keywords: Chloromonadinia; Chloromonas; evolution; Gloeomonas; molecular phylogeny; pyrenoid; ultrastructure; Volvocales , Strain(s): 447464 

836)   Nozaki, H., Onishi, K., Morita, E. 2002 Differences in pyrenoid morphology are correlated with differences in the rbcL genes of members of the Chloromonas lineage (Volvocales, Chlorophyceae). J. Mol. Evol., 55, 414-430. , Keywords: ATP synthase β-subunit (atpB) gene; Chloromonas; gene evolution; large subunit of Rubisco (rbcL) gene; morphological evolution; P700 chlorophyll a-apoprotein A2 (psaB) gene; pyrenoid; Volvocales , Strain(s): 96123144213418421425428432439446447456474522564566567569571574582653732851858861875882883968