Strain number NIES-2499  
Phylum Chlorophyta  
Class Chlorophyceae  
Scientific name Chlamydomonas eustigma Ettl  
Synonym  
Former name  
Common name Green alga  
Locality (Date of collection) Matsukawa River Takayama Vil. Nagano Japan (1992-08-**)  
Latitude / Longitude 36.65944444 / 138.52055555 
Habitat (Isolation source) Freshwater (Microfilm)  
History < Higuchi, Sumio  
Isolator (Date of isolation) Higuchi, Sumio (1992-10-**)  
Identified by Nozaki, Hisayoshi  
State of strain Subculture; Unialgal; Clonal; Axenic[2017 Dec]  
Culture condition
(Preculture condition)
Medium:  M-Allen  
Temperature:  20 C
Light intensity:  9-18 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  3 M  
Gene information Whole-genome ( BEGY01000000 ) , 18S rRNA ( AB701493 )  
Cell size (min - max)  
Organization Unicellular; Flagellate 
Characteristics Phototaxis ; Arsenic tolerance (Hirooka et al. 2017) ; Acidophilic ; Genome decoded strain (Hirooka et al. 2017)  
Other strain no.  
Remarks Axenic 
Movie  
Reference
Hirooka, S., Hirose, Y., Kanesaki, Y., Higuchi, S., Fujiwara, T., Onuma, R., Era, A., Ohbayashi, R., Uzuka, A., Nozaki, H., Yoshikawa, H., Miyagishima, SY. 2017 Acidophilic green algal genome provides insights into adaptation to an acidic environment. Proc. Natl. Acad. Sci. U. S. A., 114, E8304–E8313.
Keywords: acidic environment; acidophilic alga; comparative genomics; comparative transcriptomics; environmental adaptation
Strain(s): 2499 
PubMed: 28893987
DOI: 10.1073/pnas.1707072114

Sakurai, K., Mori, N., Sato, N. 2014 Detection and characterization of phosphatidylcholine in various strains of the genus Chlamydomonas (Volvocales, Chlorophyceae). J. Plant Res., 127, 641-650.
Keywords: Chlamydomonas; Delta 5 desaturase; Diacylglyceryl-N,N,N-trimethylhomoserine; Fatty acid; Phosphatidylcholine
Strain(s): 22022207221222152219222522302242231624622499 
PubMed: 24947506
DOI: 10.1007/s10265-014-0644-0

Yumoto, K., Kasai, F., Kawachi, M. 2013 Taxonomic re-examination of Chlamydomonas strains maintained in the NIES-Collection. Microbiol. Cult. Coll., 29, 1-12.
Strain(s): 158437438440884968102110221048173318481849220122022203220422062207220822092210221122122213221422152216221822192220222222232224222522282229223022312233223422352236223722382239224022412242224322462247224823142315231623172318231923202321232223232324246224632499257525762577257825793284 

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