Strain number NIES-2293  
Phylum Chlorophyta  
Class Chlorophyceae  
Scientific name Hamakko caudatus Nakada  
Synonym  
Former name  
Common name Green alga  
Locality (Date of collection) Kohoku-ku, Yokohama Kanagawa Japan (2003-05-12)  
Latitude / Longitude 35.503258736 / 139.596789656 
Habitat (Isolation source) Freshwater (Soil)  
History < Nakada, Takashi  
Isolator (Date of isolation) Nakada, Takashi (2004-05-11)  
Identified by Nakada, Takashi  
State of strain Subculture; Unialgal; Clonal; Axenic[2018 Jan]  
Culture condition
(Preculture condition)
Medium:  AF-6MG  
Temperature:  20 C
Light intensity:  10-17 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  1 M  
Gene information 18S rRNA ( AB451188 ) , ITS1-5.8S-ITS2 ( LC095630 ) , 26S rRNA ( LC066337 ) , atpB ( LC066347 ) , psaB ( AB451212 ) , rbcL ( AB451193 )  
Cell size (min - max)  
Organization  
Characteristics Authentic strain (Nakada, T. et al. 2009)  
Other strain no. Other strain no. : KzCl-4-1  
Remarks Axenic 
Movie  
Reference
Temraleeva, A. D., Moskalenko, S. V., Bachura, Y. M. 2017 Morphology, ecology, and 18S rDNA phylogeny of the green microalgal order Protosiphonales (Chlorophyceae, Chlorophyta). Microbiology, 86, 159-169.
Keywords: green microalgae; morphology; ecology; molecular phylogeny; polyphasic approach
Strain(s): 2293 
DOI: 10.1134/S0026261717020205

Munakata, H., Nakada, T., Nakahigashi, K., Nozaki, H., Tomita, M. 2016 Phylogenetic position and molecular chronology of a colonial green flagellate, Stephanosphaera pluvialis (Volvocales, Chlorophyceae), among unicellular algae. J. Eucaryot. Microbiol., 63, 340-348.
Keywords: Balticola; coloniality; divergence time; evolution; molecular phylogeny
Strain(s): 12314442142843243944656756957165686188796810482293256441244125 
PubMed: 26595722
DOI: 10.1111/jeu.12283

Nakada, T., Nozaki, H. 2009 Taxonomic study of two new genera of fusiform green flagellates, Tabris gen. nov. and Hamakko gen. nov. (Volvocales, Chlorophyceae). J. Phycol., 45, 482-492.
Keywords: Chlorogonium; Hamakko gen. nov.; molecular phylogeny; Tabris gen. nov.; taxonomy; ultrastructure; Volvocales
Strain(s): 122637748752760968135813591849185018692293229422954125 
PubMed: 27033826
DOI: 10.1111/j.1529-8817.2009.00652.x

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