The Microbial Culture Collection at the National Institute for Environmental Studies

Strain Data


Strain number NIES-199  
Phylum Streptophyta   Class Charophyceae 
Scientific name Closterium venus Kützing ex Ralfs  
Synonym -----  
Former name  
Common name Desmid  
Locality (Date of collection) Kathmandu Nepal (1968-**-**)  
Habitat (Isolation source) Freshwater (Pond sediment)  
History < Watanabe, Masayuki  
Isolator (Date of isolation) Ichimura, Terunobu (-----)  
Identified by Ichimura, Terunobu  
State of strain Unialgal; Clonal; Non-axenic  
Preservation state
(State of preservation)
Subculture  
Culture condition
( Preculture condition )
Medium:  CA  
Temperature: 20(20) C
Light intensity: 8-15(22-32) µmol photons/m2/sec     L/D cycle: 12L:12D
Duration: 3 M(1 M)  
Gene information 18S rRNA ( AF352236 )
Cell size (min - max)   Organization Unicellular; Non-flagellate 
Characteristics  
Other strain no. Other strain no. : N-90-48  
Remarks  

Reference
81)   Denboh, T., Hendrayanti, D., Ichimura, T. 2001 Monophyly of the genus Closterium and the order Desmidiales (Charophyceae, Chlorophyta) inferred from nuclear small subunit rDNA data. J. Phycol., 37, 1063-1072. , Keywords: Charophyceae; Closterium; desmid; molecular phylogeny; placoderm; SSU rDNA; Zygnematales , Strain(s): 52125165170174175180181185187188191194198199200271339449 

653)   Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55. , Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection , Strain(s): 19212223242526272829303132333435363738394041444546505152535455565758596061626364656667686970737475767778798081878990919293949596979899100101102103104105106107108109110111112119120122123124125127128129130131132133134135137138139144147148150151152153154155156157158159160162163164165166167168169170171172173174175176177178179180181182183185186187188189191192193194195196197198199200201202203204205206207208209210211212213214215216217224226227228229230231232241242243244245246248257259261262263266267268271287288289290294295297298299300301302303305306308309310311312313329334336337338339340341342349351359360361362375378379380382384385390394396397398415416418419421422423424425426427428429430431432433434436437438439440446447448449450451452453454455456457458459460464468469474478479480481503504505506507509510512514515522523524527528529530531532536537538539540541542543544545546564565566567568569570571572573574575576577578579580581582583584585592593594595596597598604610628630631632633634635636637638639640641642643644645646647648649650651652653654655656657658659660661662663664665666667672685686687688689690691692693694717718719720721722723724725726727728729