Strain number NIES-22  
Phylum Cyanophyta  
Class Cyanophyceae  
Scientific name Calothrix brevissima G.S.West  
Synonym  
Former name  
Common name Blue-green alga ; Cyanobacteria  
Locality (Date of collection) Palau Isl. Palau (1941-09-**)  
Latitude / Longitude  
Habitat (Isolation source) Freshwater (Soil)  
History < IAM (1983);  
Isolator (Date of isolation) Watanabe, Atsushi  
Identified by Negoro, Ken-ichiro  
State of strain Cryopreservation; Unialgal; Non-clonal; Axenic[2018 Feb]  
Culture condition
(Preculture condition)
Medium:  MDM (agar)  
Temperature:  20 (25) C
Light intensity:  4-10 (14-16) µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  4 M (14 D)  
Gene information Whole-genome ( AP018207 ) , 16S rRNA ( AB074504 ) , gyrB ( AB074768 ) , rpoC1 ( AB074791 ) , rpoD1 ( AB074818 )  
Cell size (min - max)  
Organization Filamentous 
Characteristics Nitrogen fixation  (aerobic condition) ; Chromatic adaptation  
Other strain no. Other collection strain no. : IAM M-7 (=M-249)
 
Remarks Cryopreserved; Axenic 
Movie  
Reference
Curren, E., Teruaki, Y., Kuwahara, V. S., Leong, S. C. Y. 2019 Rapid profiling of tropical marine cyanobacterial communities. Reg. Stud. Mar. Sci., 25, 100485 (article ID).
Keywords: Cyanobacteria; MinION-Oxford Nanopore sequencer; Biodiversity; 16S rRNA amplicon sequencing; Long-read sequencing; Coastal waters; Southeast Asia; Monitoring
Strain(s): 22254284321352481 
DOI: 10.1016/j.rsma.2018.100485

Seo, P-S., Yokota, A. 2003 The phylogenetic relationships of cyanobacteria inferred from 16S rRNA, gyrB, rpoC1 and rpoD1 gene sequences. J. Gen. Appl. Micobiol., 49, 191-203.
Keywords: cyanobacteria; gyrB; phylogeny; protein evolution; rpoC1; rpoD1; 16S rDNA
Strain(s): 19212225391042093209521283277 
DOI: 10.2323/jgam.49.191

Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55.
Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection
Strain(s): 19212223242526272829303132333435363738394041444546505152535455565758596061626364656667686970737475767778798081878990919293949596979899100101102103104105106107108109110111112119120122123124125127128129130131132133134135137138139144147148150151152153154155156157158159160162163164165166167168169170171172173174175176177178179180181182183185186187188189191192193194195197198199200201202203204205206207208209210211212213214215216217224226227228229230231232241242243244245246248257259261262263266267268271287288289290294295297298299300301302303305306308309310311312313329334336337338339340341342349351359360361362375378379380382384385390394396397398415416418419421422423424425426427428429430431432433434436437438439440446447448449450451452453454455456457458459460464468469474478479480481503504505506507509510512514515522523524527528529530531532536537538539540541542543544545546564565566567568569570571572573574575576577578579580581582583584585592593594595596597598604610628630631632633634635636637638639640641642643644645646647648649650651652653654655656657658659660661662663664665666667672685686687688689690691692693694717718719720721722723724725726727728729 

Hirata, K., Phunchindawan, M., Tukamoto, J., Goda, S., Miyamoto, K. 1996 Cryopreservation of microalgae using encapsulation-dehydration. CryoLetters, 17, 321-328.
Keywords: Dunaliella tertiolecta; microalgae; cryopreservation; encapsulation-dehydration
Strain(s): 8192224273244242375396422437 

Ichimura, T., Itoh, T. 1977 17. Preservation methods of microalgae (I) [17. Bisaisôrui no hozonhô (I)]. In Preservation methods of microorganisms [Biseibutsu Hozonhô], Ed. by Nei, T., University of Tokyo Press, Tokyo, p. 355-373 (in Japanese without English title).
Strain(s): 19212223242728303132333436373839404144454647484950122123127131144152173175179180181182183185198200203206217226227241266267268271295309310334338379436448450455527 

Pedersén, M., DaSilva, E. J. 1973 Simple brominated phenols in the bluegreen alga Calothrix brevissima West. Planta, 115, 83-86.
Strain(s): 22 
PubMed: 24458820
DOI: 10.1007/BF00388608

Watanabe, A. 1960 List of algal strains in collection at the Institute of Applied Microbiology, University of Tokyo. J. Gen. Appl. Microbiol., 6, 283-292.
Strain(s): 192122232425262829374749226227 

Watanabe, A. 1959 Distribution of nitrogen-fixing blue-green algae in various areas of south and east Asia. J. Gen. Appl. Microbiol., 5, 21-29.
Strain(s): 2237 
DOI: 10.2323/jgam.5.21

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