The Microbial Culture Collection at the National Institute for Environmental Studies

Strain Data


Strain number NIES-196  
Phylum Streptophyta   Class Charophyceae 
Scientific name Closterium spinosporum Hodgetts var. spinosporum  
Synonym -----  
Former name  
Common name Desmid  
Locality (Date of collection) Tsukude Tsukude Aichi Japan (1972-10-**)  
Habitat (Isolation source) Freshwater (Paddy soil)  
History < Watanabe, Masayuki  
Isolator (Date of isolation) Watanabe, Makoto M. (-----)  
Identified by Watanabe, Masayuki  
State of strain Unialgal; Clonal; Non-axenic  
Preservation state
(State of preservation)
Subculture  
Culture condition
( Preculture condition )
Medium:  CAM  
Temperature: 20(20) C
Light intensity: 8-15(22-32) µmol photons/m2/sec     L/D cycle: 12L:12D
Duration: 3 M(1 M)  
Gene information
Cell size (min - max)   Organization Unicellular; Non-flagellate 
Characteristics Homothallic  
Other strain no. Other strain no. : A-7-6  
Remarks  

Reference
632)   Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55. , Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection , Strain(s): 19212223242526272829303132333435363738394041444546505152535455565758596061626364656667686970737475767778798081878990919293949596979899100101102103104105106107108109110111112119120122123124125127128129130131132133134135137138139144147148150151152153154155156157158159160162163164165166167168169170171172173174175176177178179180181182183185186187188189191192193194195196197198199200201202203204205206207208209210211212213214215216217224226227228229230231232241242243244245246248257259261262263266267268271287288289290294295297298299300301302303305306308309310311312313329334336337338339340341342349351359360361362375378379380382384385390394396397398415416418419421422423424425426427428429430431432433434436437438439440446447448449450451452453454455456457458459460464468469474478479480481503504505506507509510512514515522523524527528529530531532536537538539540541542543544545546564565566567568569570571572573574575576577578579580581582583584585592593594595596597598604610628630631632633634635636637638639640641642643644645646647648649650651652653654655656657658659660661662663664665666667672685686687688689690691692693694717718719720721722723724725726727728729 

1256)   Watanabe, M. 1978 A taxonomic study of the Closterium calosporum complex (1). Bull. Natn. Sci. Mus., Ser. B (Bot.), 4, 133-154, pls. 1-8. , Strain(s): 163164165168169171186187188189191192193194196197271336339341