株番号 NIES-2212  
門名 Chlorophyta  
綱名 Chlorophyceae 
学名 Chlamydomonas debaryana Goroschankin  
シノニム  
以前使われていた種名 Chlamydomonas angulosa Dill  
一般名 緑藻 ; クラミドモナス  
地名(採集年月) 日本 兵庫県 神戸市  
緯度/経度 34.689404 / 135.192204 
生息環境(分離源)  
分離後の履歴 ← IAM ← UTEX (1996);  
分離者(分離年月) 坪由宏  
同定者  
株の状態 凍結保存; 無菌[2018 Feb]; 単藻; クローン  
培養条件
(前培養条件)
培地名:  C (寒天)  
温度: 20 ℃
光強度: 3-5 µmol photons/m2/sec,  明暗周期: 10L:14D
継代培養周期: 6 M  
遺伝子情報 Whole-genome ( BDDB00000000 ) , 18S rRNA ( AB701542 )  
特性 走光性 ; デンプン生産 (Toyoshima et al. 2015) ; ゲノム解読株 (Hirashima et al. 2016)  
サイズ (min - max) 7 - 12 μm  
体制 単細胞性; 鞭毛性 
その他の株番号 他のコレクション番号:IAM C-549 (=C-1); Tsubo I16; UTEX 618; CCAP 11/59; SAG 4.72
 
注意事項 凍結保存[2016 Apr]; 無菌  
動画  
文献
Hirashima, T., Toyoshima, M., Moriyama, T., Sato, N. 2018 Evolution of the phosphatidylcholine biosynthesis pathways in green algae: Combinatorial diversity of methyltransferases. J. Mol. Evol., 86, 68-76.
キーワード: Chlamydomonas; Metabolic diversity; Phosphatidylcholine biosynthesis; Phosphoethanolamine-N-methyltransferase; Phospholipid-N-methyltransferase
Strain(s): 2202220722122242 
PubMed: 29330556
DOI: 10.1007/s00239-017-9826-4

Toyoshima, M., Sato, N. 2018 Optimization of triacylglycerol and starch production in Chlamydomonas debaryana NIES-2212 with regard to light intensity and CO2 concentration. Microbiology, 164, 359-368.
キーワード: light intensity; CO2 supplement; TAG; Chlamydomonas debaryana; starch
Strain(s): 2212 
PubMed: 29458672
DOI: 10.1099/mic.0.000603

Widyaningrum, D., Iida, D., Tanabe, Y., Hayashi, Y., Kurniasih, S. D., Ohama, T. 2018 Acutely induced cell mortality in the unicellular green alga Chlamydomonas reinhardtii (Chlorophyceae) following exposure to acrylic resin nanoparticles. J. Phycol.,
Strain(s): 3514436241842143243845654557173273785810221048184821502169220122022208220922122280235224622578 
DOI: 10.1111/jpy.12798

Hirashima, T., Toyoshima, M., Moriyama, T., Nakamura, Y., Sato, N. 2017 Characterization of phosphoethanolamine-N-methyltransferases in green algae. Biochem. Biophys. Res. Commun., 488, 141-146.
キーワード: Green algal evolution; Chlamydomonas; Phosphatidylcholine biosynthesis; Phosphoethanolamine-N-methyltransferase
Strain(s): 22022207221222352242 
PubMed: 2848352
DOI: 10.1016/j.bbrc.2017.05.026

Hirashima, T., Tajima, N., Sato, N. 2016 Draft genome sequences of four species of Chlamydomonas containing phosphatidylcholine. GenomeA, 4, e01070-16 (article ID).
Strain(s): 2202220722122242 
PubMed: 27688324
DOI: 10.1128/genomeA.01070-16

Toyoshima, M., Sato N. 2015 High-level accumulation of triacylglycerol and starch in photoautotrophically grown Chlamydomonas debaryana NIES-2212. Plant Cell Physiol., 56, 2447-2456.
キーワード: Biofuel; Chlamydomonas debaryana NIES-2212; Fatty acid; Starch; Triacylglycerol
Strain(s): 2212 
PubMed: 26542110
DOI: 10.1093/pcp/pcv163

Sakurai, K., Mori, N., Sato, N. 2014 Detection and characterization of phosphatidylcholine in various strains of the genus Chlamydomonas (Volvocales, Chlorophyceae). J. Plant Res., 127, 641-650.
キーワード: Chlamydomonas; Delta 5 desaturase; Diacylglyceryl-N,N,N-trimethylhomoserine; Fatty acid; Phosphatidylcholine
Strain(s): 22022207221222152219222522302242231624622499 
PubMed: 24947506
DOI: 10.1007/s10265-014-0644-0

Yumoto, K., Kasai, F., Kawachi, M. 2013 Taxonomic re-examination of Chlamydomonas strains maintained in the NIES-Collection. Microbiol. Cult. Coll., 29, 1-12.
Strain(s): 158437438440884968102110221048173318481849220122022203220422062207220822092210221122122213221422152216221822192220222222232224222522282229223022312233223422352236223722382239224022412242224322462247224823142315231623172318231923202321232223232324246224632499257525762577257825793284 

Nozaki, H., Mori, T., Misumi, O., Matsunaga, S., Kuroiwa, T. 2006 Males evolved from the dominant isogametic mating type. Curr. Biol., 16, R1018-R1020.
Strain(s): 16111612161316141615221222132214 
PubMed: 17174904
DOI: 10.1016/j.cub.2006.11.019

Sakaguchi, T., Tsuji, T., Nakajima, A., Horikoshi, T. 1979 Accumulation of cadmium by green microalgae. Eur. J. Appl. Microbiol. Biotechnol., 8, 207-215.
Strain(s): 2212227322742279 
DOI: 10.1007/BF00506184

Nakajima, A., Horikoshi, T., Sakaguchi, T. 1979 Uptake of copper ion by green microalgae. Agric. Biol. Chem., 43, 1455-1460.
Strain(s): 2212227322742279 
DOI: 10.1080/00021369.1979.10863659

Sakaguchi, T., Horikoshi, T., Nakajima, A. 1978 Uptake of uranium from seawater by microalgae. J. Ferm. Technol., 56, 561-565.
Strain(s): 21382139221222382279 

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