株番号 NIES-2212  
門名 Chlorophyta  
綱名 Chlorophyceae 
学名 Chlamydomonas debaryana Goroschankin  
シノニム  
以前使われていた種名 Chlamydomonas angulosa Dill  
一般名 緑藻 ; クラミドモナス  
地名(採集年月) 日本 兵庫県 神戸市  
緯度/経度 34.689404 / 135.192204 
生息環境(分離源)  
分離後の履歴 ← IAM ← UTEX (1996);  
分離者(分離年月) 坪由宏  
同定者  
株の状態 継代培養; 無菌[2018 Feb]; 単藻; クローン  
培養条件
(前培養条件)
培地名:  C (寒天)  
温度: 20 ℃
光強度: 3-5 µmol photons/m2/sec,  明暗周期: 10L:14D
継代培養周期: 6 M  
遺伝子情報 Whole-genome ( BDDB00000000 ) , 18S rRNA ( AB701542 )  
特性 走光性 ; デンプン生産 (Toyoshima et al. 2015) ; ゲノム解読株 (Hirashima et al. 2016)  
サイズ (min - max) 7 - 12 μm  
体制 単細胞性; 鞭毛性 
その他の株番号 他のコレクション番号:IAM C-549 (=C-1); Tsubo I16; UTEX 618; CCAP 11/59; SAG 4.72
 
注意事項 無菌  
動画  
文献
Hirashima, T., Toyoshima, M., Moriyama, T., Sato, N. 2018 Evolution of the phosphatidylcholine biosynthesis pathways in green algae: Combinatorial diversity of methyltransferases. J. Mol. Evol., 86, 68-76.
キーワード: Chlamydomonas; Metabolic diversity; Phosphatidylcholine biosynthesis; Phosphoethanolamine-N-methyltransferase; Phospholipid-N-methyltransferase
Strain(s): 2202220722122242 
PubMed: 29330556
DOI: 10.1007/s00239-017-9826-4

Toyoshima, M., Sato, N. 2018 Optimization of triacylglycerol and starch production in Chlamydomonas debaryana NIES-2212 with regard to light intensity and CO2 concentration. Microbiology, 164, 359-368.
キーワード: light intensity; CO2 supplement; TAG; Chlamydomonas debaryana; starch
Strain(s): 2212 
PubMed: 29458672
DOI: 10.1099/mic.0.000603

Hirashima, T., Toyoshima, M., Moriyama, T., Nakamura, Y., Sato, N. 2017 Characterization of phosphoethanolamine-N-methyltransferases in green algae. Biochem. Biophys. Res. Commun., 488, 141-146.
キーワード: Green algal evolution; Chlamydomonas; Phosphatidylcholine biosynthesis; Phosphoethanolamine-N-methyltransferase
Strain(s): 22022207221222352242 
PubMed: 2848352
DOI: 10.1016/j.bbrc.2017.05.026

Hirashima, T., Tajima, N., Sato, N. 2016 Draft genome sequences of four species of Chlamydomonas containing phosphatidylcholine. GenomeA, 4, e01070-16 (article ID).
Strain(s): 2202220722122242 
PubMed: 27688324
DOI: 10.1128/genomeA.01070-16

Toyoshima, M., Sato N. 2015 High-level accumulation of triacylglycerol and starch in photoautotrophically grown Chlamydomonas debaryana NIES-2212. Plant Cell Physiol., 56, 2447-2456.
キーワード: Biofuel; Chlamydomonas debaryana NIES-2212; Fatty acid; Starch; Triacylglycerol
Strain(s): 2212 
PubMed: 26542110
DOI: 10.1093/pcp/pcv163

Sakurai, K., Mori, N., Sato, N. 2014 Detection and characterization of phosphatidylcholine in various strains of the genus Chlamydomonas (Volvocales, Chlorophyceae). J. Plant Res., 127, 641-650.
キーワード: Chlamydomonas; Delta 5 desaturase; Diacylglyceryl-N,N,N-trimethylhomoserine; Fatty acid; Phosphatidylcholine
Strain(s): 22022207221222152219222522302242231624622499 
PubMed: 24947506
DOI: 10.1007/s10265-014-0644-0

Yumoto, K., Kasai, F., Kawachi, M. 2013 Taxonomic re-examination of Chlamydomonas strains maintained in the NIES-Collection. Microbiol. Cult. Coll., 29, 1-12.
Strain(s): 1584374384408849681021102210481733184818492201220222032204220622072208220922102211221222132214221522162218221922202222222322242225222822292230223122332234223522362237223922402241224222432246224722482314231523162317231823192320232123222323232424622463249925752576257725782579 

Nozaki, H., Mori, T., Misumi, O., Matsunaga, S., Kuroiwa, T. 2006 Males evolved from the dominant isogametic mating type. Curr. Biol., 16, R1018-R1020.
Strain(s): 16111612161316141615221222132214 
PubMed: 17174904
DOI: 10.1016/j.cub.2006.11.019

Sakaguchi, T., Tsuji, T., Nakajima, A., Horikoshi, T. 1979 Accumulation of cadmium by green microalgae. Eur. J. Appl. Microbiol. Biotechnol., 8, 207-215.
Strain(s): 2212227322742279 
DOI: 10.1007/BF00506184

Nakajima, A., Horikoshi, T., Sakaguchi, T. 1979 Uptake of copper ion by green microalgae. Agric. Biol. Chem., 43, 1455-1460.
Strain(s): 2212227322742279 
DOI: 10.1080/00021369.1979.10863659

Sakaguchi, T., Horikoshi, T., Nakajima, A. 1978 Uptake of uranium from seawater by microalgae. J. Ferm. Technol., 56, 561-565.
Strain(s): 2138213922122279 

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