Strain number NIES-2173  
Phylum Chlorophyta  
Class Trebouxiophyceae  
Scientific name Chlorella sorokiniana Shihira & R.W.Krauss  
Synonym  
Former name Chlorella vulgaris Beijerinck  
Common name Green alga  
Locality (Date of collection)  
Latitude / Longitude  
Habitat (Isolation source)  
History < IAM (2007) < Tsuzuki, Mikio (1988) < Avramova, S. T. (1982) < probably Semenenko  
Isolator (Date of isolation)  
Identified by Hoshina, Ryo (Reidentify)  
State of strain Cryopreservation; Unialgal; Clonal; Axenic[2018 Feb]  
Culture condition
(Preculture condition)
Medium:  C (agar)  
Temperature:  20 C
Light intensity:  8-15 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  3 M  
Gene information 18S rRNA ( AB080308 ) , actin-1 ( AB080312 ) , actin-2 ( AB080313 ) , 18S-ITS1-5.8S-ITS2-28S ( AB731601 AB731602 )  
Cell size (min - max)  
Organization Unicellular 
Characteristics Starch production (Miyachi et al. 1986)  
Other strain no. Other collection strain no. : IAM C-536; IPPAS C-3
 
Remarks Cryopreserved; Axenic 
Movie  
Reference
Mathiot, C., Ponge, P., Gallard, B., Sassi, J.-F., Delrue, F., Le Moigne, N. 2019 Microalgae starch-based bioplastics: screening of ten strains and plasticization of unfractionated microalgae by extrusion. Carbohydr. Polym., 208/, 142-151.
Keywords: Microalgae; Bioplastics; Starch; Plasticization; Extrusion
Strain(s): 942272152217321952280 
DOI: 10.1016/j.carbpol.2018.12.057

Kimura, S., Yamada, T., Ban, S., Koyama, M., Toda, T. 2019 Nutrient removal from anaerobic digestion effluents of aquatic macrophytes with the green alga, Chlorella sorokiniana. Biochem. Eng. J., 142, 170-177.
Keywords: Chlorella sorokiniana; anaerobic digestion effluent; maximum algal yield; nutrient removal rate; aquatic macrophytes
Strain(s): 2173 
DOI: 10.1016/j.bej.2018.12.001

Hayashida, G., Schneider, C., Espíndola, L., Arias, D., Riquelme, C., Wulff-Zottele, C., Díaz-Palma, P., Rivas, M. 2017 Characterization of a Chlorophyta microalga isolated from a microbial mat in Salar de Atacama (northern Chile) as a potential source of compounds for biotechnological applications. Phycol. Res., 65, 202-211.
Keywords: Altiplane; Chlorella sorokiniana; extreme tolerant; microalgae; microbial mat; oleaginous strain, polyunsaturated; fatty acid; stearic acid
Strain(s): 2173 
DOI: 10.1111/pre.12176

Huang, Y.-T., Su, C.-P. 2014 High lipid content and productivity of microalgae cultivating under elevated carbon dioxide. Int. J. Environ. Sci. Technol., 11, 703-710.
Keywords: Biodiesel; Chlorella; Global warming; Light intensity
Strain(s): 2173 
DOI: 10.1007/s13762-013-0251-y

Mizuno, Y., Sato, A., Watanabe, K., Hirata, A., Takeshita, T., Ota, S., Sato, N., Zachleder, V., Tsuzuki, M., Kawano, S. 2013 Sequential accumulation of starch and lipid induced by sulfur deficiency in Chlorella and Parachlorella species. Bioresour. Technol., 129, 150-155.
Keywords: Sulfur deficiency; Chlorella spp.; Parachlorella kessleri; Starch; Lipids
Strain(s): 21692173 
PubMed: 23238344
DOI: 10.1016/j.biortech.2012.11.030

Hoshina, R., Fujiwara, Y. 2013 Molecular characterization of Chlorella cultures of the National Institute for Environmental Studies culture collection with description of Micractinium inermum sp. nov., Didymogenes sphaerica sp. nov., Didymogenes soliella sp. nov., (Chlorellaceae, Trebouxiophyceae). Phycol. Res., 61, 124-132.
Keywords: Chlorella; Didymogenes; group 1 intron; internal transcribed spacer 2; Micractinium; species concept
Strain(s): 2167217121732330 
DOI: 10.1111/pre.12010

Yamamoto, M., Nozaki, H., Miyazawa, Y., Koide, T., Kawano, S. 2003 Relationship between presence of a mother cell wall and speciation in the unicellular microalga Nannochloris (Chlorophyta). J. Phycol., 39, 172-184.
Keywords: 18S rRNA; actin gene; autosporulation; binary fission; budding; Chlorella; Chlorophyta; molecular phylogeny; TBR, tree bisection reconnection
Strain(s): 21602169217321864016 
DOI: 10.1046/j.1529-8817.2003.02052.

Tsuzuki, M., Miyachi, S. 1989 The function of carbonic anhydrase in aquatic photosynthesis. Aquatic Botany, 34, 85-104.
Strain(s): 20932138216021732235 

Bürger, J., Miyachi, S., Galland, P., Senger, H. 1988 Quantum requirements of photosynthetic oxygen evolution and 77K fluorescence emission spectra in unicellular green algae grown under low- and high-CO2-conditions. Botanica Acta, 101, 229-232.
Strain(s): 21732235 
DOI: 10.1111/j.1438-8677.1988.tb00037.x

Miyachi, S., Tsuzuki, M., Maruyama, I., Ganter, M., Miyachi, S. 1986 Effects of CO2 concentration during growth on the intracellular structure of Chlorella and Scenedesmus (Chlorophyta). J. Phycol., 22, 313-319.
Keywords: Chlorella; CO2 concentration; electron microscopy; pyrenoid starch; Scenedesmus
Strain(s): 68721572160217021732280 

Aizawa, K., Miyachi, S. 1986 Carbonic anhydrase and CO2 concentrating mechanisms in microalgae and cyanobacteria. FEMS Microbiol. Lett., 39, 215-233.
Keywords: aquatic algae; cyanobacteria; photoautotrophs; photorespiration; photosynthesis; transport
Strain(s): 2093216021732235 
DOI: 10.1111/j.1574-6968.1986.tb01860.x

Miyachi, S., Tsuzuki, M., Yagawa, Y. 1985 Carbonic anhydrase in various microalgae. In Inorganic Carbon Uptake by Aquatic Photosynthetic Organisms, Eds. by Lucas, W. J. & Berry, J. A., American Society of Plant Physiologists, Rockville, Md, p. 145-154.
Strain(s): 209321602173 

Miyachi, S., Miyachi, S. 1985 Ammonia induces starch degradation in Chlorella cells. Plant Cell Physiol., 26, 245-252.
Keywords: ammonia effect; Chlorella; dark CO2 fixation; starch degradation; sucrose formation
Strain(s): 6872157216021702173 

Tsuzuki, M., Shimamoto, T., Yang, S. Y., Miyachi, S. 1984 Diversity in intracellular locality, nature, and function of carbonic anhydrase in verious plants. Ann. NY Acad. Sci., 429, 238-240.
Strain(s): 6872160216121622170217322352280 
DOI: 10.1111/j.1749-6632.1984.tb12341.x

Miyachi, S., Tsuzuki, M., Avramova, S. T. 1983 Utilization modes of inorganic carbon for photosynthesis in various species of Chlorella. Plant Cell Physiol., 24, 441-451.
Keywords: bicarbonate fixation; carbonic anhydrase; Chlorella; photosynthetic CO2-fixation
Strain(s): 6872093215721602173 

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