The Microbial Culture Collection at the National Institute for Environmental Studies

Strain Data


Strain number NIES-450  
Phylum Streptophyta   Class Charophyceae 
Scientific name Closterium praelongum Brébisson var. brevius (Nordstedt) Krieger  
Synonym -----  
Former name  
Common name Desmid  
Locality (Date of collection) Nawakot Nepal (1965-10-12)  
Habitat (Isolation source) Freshwater (Pond sediment)  
History < IAM (1983);  
Isolator (Date of isolation) Ichimura, Terunobu (-----)  
Identified by Ichimura, Terunobu  
State of strain Unialgal; Clonal; Axenic[2012 Dec]  
Preservation state
(State of preservation)
Subculture  
Culture condition
( Preculture condition )
Medium:  CA  
Temperature: 20(20) C
Light intensity: 8-15(22-32) µmol photons/m2/sec     L/D cycle: 12L:12D
Duration: 3 M(1 M)  
Gene information
Cell size (min - max) - 160 μm   Organization Unicellular; Non-flagellate 
Characteristics Homothallic  
Other strain no. Other collection strain no. : IAM C-447 (=C-578)
Other strain no. : N-12-3  
Remarks  

Reference
270)   Ichimura, T. 1973 Culture of microalgae (6). Iden, 27, 97-101 (in Japanese). , Strain(s): 66173175179180181183198262338450 

271)   Ichimura, T. 1973 The life cycle and its control in some species of Closterium, with special reference to the biological species problems. Thesis D. Sci., University of Tokyo, 69 pp., 11 tables, 40 figs. , Strain(s): 666768124125127174175176177179180181182183185198202262337338450451 

277)   Ichimura, T., Itoh, T. 1977 17. Preservation methods of microalgae (I) [17. Bisaisôrui no hozonhô (I)]. In Preservation methods of microorganisms [Biseibutsu Hozonhô], Ed. by Nei, T., University of Tokyo Press, Tokyo, p. 355-373 (in Japanese without English title). , Strain(s): 19212223242728303132333436373839404144454647484950122123127131144152173175179180181182183185198200203206217226227241266267268271295309310334338379436448450455527 

653)   Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55. , Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection , Strain(s): 19212223242526272829303132333435363738394041444546505152535455565758596061626364656667686970737475767778798081878990919293949596979899100101102103104105106107108109110111112119120122123124125127128129130131132133134135137138139144147148150151152153154155156157158159160162163164165166167168169170171172173174175176177178179180181182183185186187188189191192193194195196197198199200201202203204205206207208209210211212213214215216217224226227228229230231232241242243244245246248257259261262263266267268271287288289290294295297298299300301302303305306308309310311312313329334336337338339340341342349351359360361362375378379380382384385390394396397398415416418419421422423424425426427428429430431432433434436437438439440446447448449450451452453454455456457458459460464468469474478479480481503504505506507509510512514515522523524527528529530531532536537538539540541542543544545546564565566567568569570571572573574575576577578579580581582583584585592593594595596597598604610628630631632633634635636637638639640641642643644645646647648649650651652653654655656657658659660661662663664665666667672685686687688689690691692693694717718719720721722723724725726727728729