Strain number NIES-449  
Phylum Streptophyta  
Class Charophyceae  
Scientific name Closterium pleurodermatum W. & G.S.West  
Synonym  
Former name  
Common name Desmid  
Locality (Date of collection) Iriomote Isl. Hoshidachi Okinawa Japan (1973-03-25)  
Latitude / Longitude  
Habitat (Isolation source) Freshwater (Paddy soil)  
History < IAM (1983);  
Isolator (Date of isolation) Ichimura, Terunobu (1973-12-05)  
Identified by Ichimura, Terunobu  
State of strain Subculture; Unialgal; Clonal; Non-axenic  
Culture condition
(Preculture condition)
Medium:  AF-6  
Temperature:  20 (25) C
Light intensity:  7-8 (12) µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  3 M (21 D)  
Gene information 18S rRNA ( AF352238 )  
Cell size (min - max)  
Organization Unicellular 
Characteristics  
Other strain no. Other collection strain no. : IAM C-518
Other strain no. : R-11-20  
Remarks  
Movie  
Reference
Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55.
Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection
Strain(s): 19212223242526272829303132333435363738394041444546505152535455565758596061626364656667686970737475767778798081878990919293949596979899100101102103104105106107108109110111112119120122123124125127128129130131132133134135137138139144147148150151152153154155156157158159160162163164165166167168169170171172173174175176177178179180181182183185186187188189191192193194195197198199200201202203204205206207208209210211212213214215216217224226227228229230231232241242243244245246248257259261262263266267268271287288289290294295297298299300301302303305306308309310311312313329334336337338339340341342349351359360361362375378379380382384385390394396397398415416418419421422423424425426427428429430431432433434436437438439440446447448449450451452453454455456457458459460464468469474478479480481503504505506507509510512514515522523524527528529530531532536537538539540541542543544545546564565566567568569570571572573574575576577578579580581582583584585592593594595596597598604610628630631632633634635636637638639640641642643644645646647648649650651652653654655656657658659660661662663664665666667672685686687688689690691692693694717718719720721722723724725726727728729 

Denboh, T., Hendrayanti, D., Ichimura, T. 2001 Monophyly of the genus Closterium and the order Desmidiales (Charophyceae, Chlorophyta) inferred from nuclear small subunit rDNA data. J. Phycol., 37, 1063-1072.
Keywords: Charophyceae; Closterium; desmid; molecular phylogeny; placoderm; SSU rDNA; Zygnematales
Strain(s): 52125165170174175180181185187188191194198199200271339449 
DOI: 10.1046/j.1529-8817.2001.00146.x

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