Strain number NIES-1849  
Phylum Chlorophyta  
Class Chlorophyceae  
Scientific name Chlamydomonas perpusilla (Korshikov) Gerloff var. perpusilla  
Synonym Chlamydomonas minima Korshikov  
Former name  
Common name Green alga  
Locality (Date of collection) Sakata-ga-ike Pond Ohtake, Narita Chiba Japan (2003-07-23)  
Latitude / Longitude 35.81833333 / 140.27388888 
Habitat (Isolation source) Freshwater (Pond sediment)  
History < Nakada, Takashi  
Isolator (Date of isolation) Nakada, Takashi (2003-08-18)  
Identified by Nakada, Takashi  
State of strain Cryopreservation; Unialgal; Clonal; Axenic[2017 Dec]  
Culture condition
(Preculture condition)
Medium:  MG  
Temperature:  20 C
Light intensity:  7-13 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  2 M  
Gene information 18S rRNA ( AB290339 AB753037 ) , psaB ( AB451199 ) , rbcL ( AB360754 )  
Cell size (min - max)  
Organization Unicellular; Flagellate 
Characteristics Phototaxis  
Other strain no. Other strain no. : SkCr-10  
Remarks Axenic; Cryopreserved 
Movie  
Reference
Nakada, T. 2018 Methods for supporting cover slip during light microscopy of unfixed microalgae, using clothing fibers or adhesive tapes. Jpn. J. Phycol. (Sôrui), 66, 157-163 (in Japanese with English summary).
Keywords: adhesive tape; chemical fiber; light microscopy; microalgae; slide preparation; unfixed cells
Strain(s): 5441849 

Yumoto, K., Kasai, F., Kawachi, M. 2013 Taxonomic re-examination of Chlamydomonas strains maintained in the NIES-Collection. Microbiol. Cult. Coll., 29, 1-12.
Strain(s): 158437438440884968102110221048173318481849220122022203220422062207220822092210221122122213221422152216221822192220222222232224222522282229223022312233223422352236223722382239224022412242224322462247224823142315231623172318231923202321232223232324246224632499257525762577257825793284 

Nakada, T., Nozaki, H. 2009 Taxonomic study of two new genera of fusiform green flagellates, Tabris gen. nov. and Hamakko gen. nov. (Volvocales, Chlorophyceae). J. Phycol., 45, 482-492.
Keywords: Chlorogonium; Hamakko gen. nov.; molecular phylogeny; Tabris gen. nov.; taxonomy; ultrastructure; Volvocales
Strain(s): 122637748752760968135813591849185018692293229422954125 
PubMed: 27033826
DOI: 10.1111/j.1529-8817.2009.00652.x

Nakada, T., Nozaki, H. 2007 Two species of Chlamydomonas (Volvocales, Chlorophyceae) new to Japan. J. Jpn. Bot., 82, 179-189.
Keywords: Chlamydomonas perpusilla; Chlamydomonas pumilio; culture strain; taxonomy
Strain(s): 184818491850 

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