Strain number NIES-1848  
Phylum Chlorophyta  
Class Chlorophyceae  
Scientific name Chlamydomonas perpusilla (Korshikov) Gerloff var. perpusilla  
Synonym Chlamydomonas minima Korshikov  
Former name  
Common name Green alga  
Locality (Date of collection) Sakata-ga-ike Pond Ohtake, Narita Chiba Japan (2003-07-23)  
Latitude / Longitude 35.81944444 / 140.27444444 
Habitat (Isolation source) Freshwater (Pond sediment)  
History < Nakada, Takashi  
Isolator (Date of isolation) Nakada, Takashi (2003-08-18)  
Identified by Nakada, Takashi  
State of strain Cryopreservation; Unialgal; Clonal; Axenic[2017 Dec]  
Culture condition
(Preculture condition)
Medium:  MG  
Temperature:  20 C
Light intensity:  7-13 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  2 M  
Gene information 18S rRNA ( AB753036 )  
Cell size (min - max)  
Organization Unicellular; Flagellate 
Characteristics Phototaxis  
Other strain no. Other strain no. : SkCl-3  
Remarks Axenic; Cryopreserved 
Movie  
Reference
Widyaningrum, D., Iida, D., Tanabe, Y., Hayashi, Y., Kurniasih, S. D., Ohama, T. 2019 Acutely induced cell mortality in the unicellular green alga Chlamydomonas reinhardtii (Chlorophyceae) following exposure to acrylic resin nanoparticles. J. Phycol., 55, 118-133.
Keywords: abnormal swimming behavior; cell mortality; cell wall damage; Chlamydomonas rein-hardtii; poly(isobutyl-cyanoacrylate) nanoparticles; ROS
Strain(s): 3514436241842143243845654557173273785810221048184821502169220122022208220922122280235224622578 
PubMed: 30304548
DOI: 10.1111/jpy.12798

Yumoto, K., Kasai, F., Kawachi, M. 2013 Taxonomic re-examination of Chlamydomonas strains maintained in the NIES-Collection. Microbiol. Cult. Coll., 29, 1-12.
Strain(s): 158437438440884968102110221048173318481849220122022203220422062207220822092210221122122213221422152216221822192220222222232224222522282229223022312233223422352236223722382239224022412242224322462247224823142315231623172318231923202321232223232324246224632499257525762577257825793284 

Nakada, T., Nozaki, H. 2007 Two species of Chlamydomonas (Volvocales, Chlorophyceae) new to Japan. J. Jpn. Bot., 82, 179-189.
Keywords: Chlamydomonas perpusilla; Chlamydomonas pumilio; culture strain; taxonomy
Strain(s): 184818491850 

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