Strain number NIES-180  
Phylum Streptophyta  
Class Charophyceae  
Scientific name Closterium gracile Brébisson ex Ralfs  
Synonym Closterium limneticum var. tenue Lemmermann 1899; Closterium gracile var. tenue (Lemmermann) West & G.S.West 1902; Closterium gracile var. elongatum West & G.S.West 1904; Closterium gracile f. tenue (Lemmermann) Kossinskaja 1960  
Former name  
Common name Desmid  
Locality (Date of collection) Kathmandu Nepal (1968-05-18)  
Latitude / Longitude  
Habitat (Isolation source) Freshwater (Sediment)  
History < IAM (1983);  
Isolator (Date of isolation) Ichimura, Terunobu  
Identified by Ichimura, Terunobu  
State of strain Subculture; Unialgal; Clonal; Non-axenic  
Culture condition
(Preculture condition)
Medium:  CA  
Temperature:  20 (20) C
Light intensity:  4-8 (13-21) µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  3 M (1 M)  
Gene information 18S rRNA ( AF352227 AF352237 )  
Cell size (min - max)  
Organization Unicellular 
Characteristics Heterothallic: Mating type (−) ; Crosses with NIES-179  
Other strain no. Other collection strain no. : IAM C-445 (=C-571)
Other strain no. : N-90-59  
Remarks  
Movie  
Reference
Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55.
Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection
Strain(s): 19212223242526272829303132333435363738394041444546505152535455565758596061626364656667686970737475767778798081878990919293949596979899100101102103104105106107108109110111112119120122123124125127128129130131132133134135137138139144147148150151152153154155156157158159160162163164165166167168169170171172173174175176177178179180181182183185186187188189191192193194195197198199200201202203204205206207208209210211212213214215216217224226227228229230231232241242243244245246248257259261262263266267268271287288289290294295297298299300301302303305306308309310311312313329334336337338339340341342349351359360361362375378379380382384385390394396397398415416418419421422423424425426427428429430431432433434436437438439440446447448449450451452453454455456457458459460464468469474478479480481503504505506507509510512514515522523524527528529530531532536537538539540541542543544545546564565566567568569570571572573574575576577578579580581582583584585592593594595596597598604610628630631632633634635636637638639640641642643644645646647648649650651652653654655656657658659660661662663664665666667672685686687688689690691692693694717718719720721722723724725726727728729 

Denboh, T., Hendrayanti, D., Ichimura, T. 2001 Monophyly of the genus Closterium and the order Desmidiales (Charophyceae, Chlorophyta) inferred from nuclear small subunit rDNA data. J. Phycol., 37, 1063-1072.
Keywords: Charophyceae; Closterium; desmid; molecular phylogeny; placoderm; SSU rDNA; Zygnematales
Strain(s): 52125165170174175180181185187188191194198199200271339449 
DOI: 10.1046/j.1529-8817.2001.00146.x

Ichimura, T., Itoh, T. 1977 17. Preservation methods of microalgae (I) [17. Bisaisôrui no hozonhô (I)]. In Preservation methods of microorganisms [Biseibutsu Hozonhô], Ed. by Nei, T., University of Tokyo Press, Tokyo, p. 355-373 (in Japanese without English title).
Strain(s): 19212223242728303132333436373839404144454647484950122123127131144152173175179180181182183185198200203206217226227241266267268271295309310334338379436448450455527 

Ichimura, T. 1973 The life cycle and its control in some species of Closterium, with special reference to the biological species problems. Thesis D. Sci., University of Tokyo, 69 pp., 11 tables, 40 figs.
Strain(s): 666768124125127174175176177179180181182183185198202262337338450451 

Ichimura, T. 1973 Culture of microalgae (6). Iden, 27, 97-101 (in Japanese).
Strain(s): 66173175179180181183198262338450 

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