The Microbial Culture Collection at the National Institute for Environmental Studies

Strain Data


Strain number NIES-165  
Phylum Streptophyta   Class Charophyceae 
Scientific name Closterium calosporum Wittrock var. galiciense Gutwinski  
Synonym -----  
Former name  
Common name Desmid  
Locality (Date of collection) Iriomote Isl. Hosidachi Okinawa Japan (1973-03-25)  
Habitat (Isolation source) Freshwater (Paddy soil)  
History < IAM (1983);  
Isolator (Date of isolation) Ichimura, Terunobu (1973-10-29)  
Identified by Watanabe, Masayuki  
State of strain Unialgal; Clonal; Axenic[2012 Dec]  
Preservation state
(State of preservation)
Subculture  
Culture condition
( Preculture condition )
Medium:  CA  
Temperature: 20(25) C
Light intensity: 8-15(60-70) µmol photons/m2/sec     L/D cycle: 12L:12D
Duration: 3 M(1 M)  
Gene information 18S rRNA ( AF352239 )
Cell size (min - max)   Organization Unicellular; Non-flagellate 
Characteristics Heterothallic: Mating type(+); Crosses with NIES-163, 166 and 168  
Other strain no. Other collection strain no. : IAM C-457
Other strain no. : R-11-6  
Remarks  

Reference
79)   Denboh, T., Hendrayanti, D., Ichimura, T. 2001 Monophyly of the genus Closterium and the order Desmidiales (Charophyceae, Chlorophyta) inferred from nuclear small subunit rDNA data. J. Phycol., 37, 1063-1072. , Keywords: Charophyceae; Closterium; desmid; molecular phylogeny; placoderm; SSU rDNA; Zygnematales , Strain(s): 52125165170174175180181185187188191194198199200271339449 

279)   Ichimura, T., Watanabe, M. 1974 The Closterium calosporum complex from the Ryukyu Islands - Variation and taxonomical problems. Mem. Natn. Sci. Mus. Tokyo, 7, 89-102, pls. 13-14. , Strain(s): 163164165168186187194271339340341 

635)   Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55. , Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection , Strain(s): 19212223242526272829303132333435363738394041444546505152535455565758596061626364656667686970737475767778798081878990919293949596979899100101102103104105106107108109110111112119120122123124125127128129130131132133134135137138139144147148150151152153154155156157158159160162163164165166167168169170171172173174175176177178179180181182183185186187188189191192193194195196197198199200201202203204205206207208209210211212213214215216217224226227228229230231232241242243244245246248257259261262263266267268271287288289290294295297298299300301302303305306308309310311312313329334336337338339340341342349351359360361362375378379380382384385390394396397398415416418419421422423424425426427428429430431432433434436437438439440446447448449450451452453454455456457458459460464468469474478479480481503504505506507509510512514515522523524527528529530531532536537538539540541542543544545546564565566567568569570571572573574575576577578579580581582583584585592593594595596597598604610628630631632633634635636637638639640641642643644645646647648649650651652653654655656657658659660661662663664665666667672685686687688689690691692693694717718719720721722723724725726727728729 

1261)   Watanabe, M. 1978 A taxonomic study of the Closterium calosporum complex (1). Bull. Natn. Sci. Mus., Ser. B (Bot.), 4, 133-154, pls. 1-8. , Strain(s): 163164165168169171186187188189191192193194196197271336339341 

1262)   Watanabe, M. 1979 A taxonomic study of the Closterium calosporum complex (2). Bull. Natn. Sci. Mus., Ser. B (Bot.), 5, 1-23, pls. 9-11. , Strain(s): 163164165168169186187188189191192194195197271339340341