Strain number NIES-81  
Phylum Cyanophyta  
Class Cyanophyceae  
Scientific name Aphanizomenon flos-aquae (L.) Ralfs ex Bornet & Flahault f. gracile (Lemmermann) Elenkin  
Synonym  
Former name  
Common name Blue-green alga ; Cyanobacteria  
Locality (Date of collection) Lake Kasumigaura Ibaraki Japan (1978-01-24)  
Latitude / Longitude 36.0428477398 / 140.373233439 
Habitat (Isolation source) Freshwater (Lake water)  
History < TAC;  
Isolator (Date of isolation) Watanabe, Masayuki (1978-02-15)  
Identified by Watanabe, Masayuki  
State of strain Cryopreservation; Unialgal; Clonal; Axenic[2018 Feb]  
Culture condition
(Preculture condition)
Medium:  CB  
Temperature:  25 C
Light intensity:  20-25 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  20 D  
Gene information Whole-genome ( AZYY00000000 ) , 16S rRNA ( AF317632 AJ293131 AY038035 ) , rbcL, rbcX ( AJ293170 ) , ITS1 ( AJ293213 )  
Cell size (min - max) - 5 μm  
Organization Filamentous 
Characteristics Cyanobacterial water bloom (aoko) ; Offensive odor ; Draft genome sequence (Cao et al. 2014)  
Other strain no. Other strain no. : TAC 1 (K-TAN-1)  
Remarks Cryopreserved; Axenic 
Movie  
Reference
Lin, W., Zhao, D., Luo, J. 2018 Distribution of alkaline phosphatase genes in cyanobacteria and the role of alkaline phosphatase on the acquisition of phosphorus from dissolved organic phosphorus for cyanobacterial growth. J. Appl. Phycol., 30, 839-850.
Keywords: Alkaline; phosphatase; Cyanobacteria; Dissolved organic phosphorus; Distribution
Strain(s): 303981213025493756 
DOI: 10.1007/s10811-017-1267-3

Driscoll, C. B., Meyer, K. A., Šulčius, S., Brown, N. M., Dick, G. J., Cao, H., Gasiūnas, G., Timinskas, A., Yin, Y., Landry, Z. C., Otten, T. G., Davis, T. W., Watson, S. B., Dreher, T. W. 2018 A closely-related clade of globally distributed bloom-forming cyanobacteria within the Nostocales. Harmful Algae, 77, 93-107.
Keywords: Genomics; Metagenomics; Filamentous cyanobacteria
Strain(s): 81 
PubMed: 30005805
DOI: 10.1016/j.hal.2018.05.009

Kerfeld, CA., Melnicki, MR., Sutter M., Dominguez-Martin, MA. 2017 Structure, function and evolution of the cyanobacterial orange carotenoid protein and its homologs. New Phytol., 215, 937-951.
Keywords: carotenoids; carotenoproteins; cyanobacteria; nonphotochemical quenching; photoprotection; phylogenomics; protein structure; singlet oxygen (1O2) quenching
Strain(s): 3981843 
PubMed: 28675536
DOI: 10.1111/nph.14670

Chiu, Y.-T., Yen, H.-K., Lin, T.-F. 2016 An alternativemethodtoquantify2-MIB producing cyanobacteriain in drinking water reservoirs: Method development and field applications. Environ. Res., 151, 618-627.
Keywords: Cyanobacteria; 2-methylisoborneol(2-MIB); 2-MIB-synthesis gene(mibC gene); Tasteandodor; Real-timePCR
Strain(s): 8151282490521042116 
PubMed: 27607443
DOI: 10.1016/j.envres.2016.08.034

Cao, H., Shimura, Y., Kawachi, M., Yin, Y. 2014 Draft genome sequence of the toxic bloom-forming cyanobacterium Aphanizomenon flos-aquae NIES-81. GenomeA, 2, e00044-14 (article ID).
Strain(s): 81 
PubMed: 24526634
DOI: 10.1128/genomeA.00044-14

Wacklin, P., Hoffmann, L., Komárek, J. 2009 Nomenclatural validation of the genetically revised cyanobacterial genus Dolichospermum (Ralfs ex Bornet et Flahault) comb. nova. Fottea, 9, 59-64.
Keywords: Cyanobacteria; Anabaena; Dolichospermum; taxonomy; nomenclatoric validation
Strain(s): 81 

Koskenniemi, K., Lyra, C., Rajaniemi-Wacklin, P., Jokela, J., Sivonen, K. 2007 Quantitative real-time PCR detection of toxic Nodularia cyanobacteria in the Baltic Sea. Appl. Environ. Microbiol., 73, 2173-2179.
Strain(s): 8189102 
PubMed: 17277219
DOI: 10.1128/AEM.02746-06

Hosoya, R., Hamana, K., Isobe, M., Yokota, A. 2005 Polyamine distribution profiles within Cyanobacteria. Microbiol. Cult. Coll., 21, 3-8.
Keywords: cyanobacteria; homospermidine; polyamine; spermidine
Strain(s): 41507378798081512515592593610846847 

Hirabayashi, R., Imamura, N. 2003 Action mechanism of a selective anti-cyanobacterial compound, argimicin A. J. Antibiotics, 56, 154-159.
Strain(s): 414581102204230298592 
DOI: 10.7164/antibiotics.56.154

Yamaguchi, T., Kobayashi, Y., Adachi, K., Imamura, N. 2003 Argimicins B and C, new anti-cyanobacterial compounds produced by Sphingomonas sp. M-17. J. Antibiot., 56, 655-657.
Strain(s): 4581102230298592 

Jung, J.-M., Lee, Y.-J., Park, H.-K., Jung, E.-Y., Joo, G.-J. 2003 The analysis of cyanobacterial neurotoxins by high-performance liquid chromatography-mass spectrometry. Algae, 18, 233-238.
Keywords: anatoxin-a; Aphanizomenon flos-aquae; cyanobacterial toxins; LC-MS; saxitoxin
Strain(s): 81 
DOI: 10.4490/ALGAE.2003.18.3.23

Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55.
Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection
Strain(s): 19212223242526272829303132333435363738394041444546505152535455565758596061626364656667686970737475767778798081878990919293949596979899100101102103104105106107108109110111112119120122123124125127128129130131132133134135137138139144147148150151152153154155156157158159160162163164165166167168169170171172173174175176177178179180181182183185186187188189191192193194195197198199200201202203204205206207208209210211212213214215216217224226227228229230231232241242243244245246248257259261262263266267268271287288289290294295297298299300301302303305306308309310311312313329334336337338339340341342349351359360361362375378379380382384385390394396397398415416418419421422423424425426427428429430431432433434436437438439440446447448449450451452453454455456457458459460464468469474478479480481503504505506507509510512514515522523524527528529530531532536537538539540541542543544545546564565566567568569570571572573574575576577578579580581582583584585592593594595596597598604610628630631632633634635636637638639640641642643644645646647648649650651652653654655656657658659660661662663664665666667672685686687688689690691692693694717718719720721722723724725726727728729 

Yuki, Y., Yoshida, T., Hiroishi, S. 2002 Molecular detection of the toxic cyanobacteria [Yûdoku aoko no bunshi-shikibetsu to yosatsu he-no ôyô]. In Series of Fisheries Science Vol. 143, Prevention and Extermination Strategies of Harmful Algal Blooms [Suisan-gaku shirîzu 134, Yûgai-yûdoku sôrui burûmu no yobô to kujo] , Eds. by Hiroishi, S., Imai, I. & Ishimaru, T., Koseisha-Koseikaku, Tokyo, p. 43-53 (in Japanese without English title).
Strain(s): 738187888990919899100101102104105106108109110111112204230298299604 

Gugger, M., Lyra, C., Henriksen, P., Coute, A., Humbert, J.-F., Sivonen, K. 2002 Phylogenetic comparison of the cyanobacterial genera Anabena and Aphanizomenon. Int. J. Syst. Evol. Microbiol., 52, 1867-1880.
Keywords: Anabaena; Aphanizomenon; 16S rDNA; 16S-23S rDNA; RubisCO spacer
Strain(s): 81 
DOI: 10.1099/00207713-52-5-1867

Lyra, C., Suomalainen, S., Gugger, M., Vezie, C., Sundman, P., Paulin, L., Sivonen, K. 2001 Molecular characterization of planktic cyanobacteria of Anabaena, Aphanizomenon, Microcystis and Planktothrix genera. Int. J. Syst. Evol. Microbiol., 51, 13-526.
Keywords: cyanobacteria; 16S rRNA gene; RFLP; sequencing; REP; ERIC
Strain(s): 41447379818998102104 
DOI: 10.1099/00207713-51-2-513

Murakami, M., Suzuki, S., Itou, Y., Kodani, S., Ishida, K. 2000 New anabaenopeptins, potent carboxypeptidase-A inhibitors from the cyanobacterium Aphanizomenon flos-aquae. J. Nat. Prod., 63, 1280-1282.
Strain(s): 81 

Tillett, D., Neilan, B. A. 2000 Xanthogenate nucleic acid isolation from cultured and environmental cyanobacteria. J. Phycol., 36, 251-258.
Keywords: cyanobacteria; DNA extraction; nucleic acids; PCR; xanthogenate
Strain(s): 81102107 
DOI: 10.1046/j.1529-8817.2000.99079.x

Neilan, B. A., Dittmann, E., Rouhiainen, L., Bass, R. A., Schaub, V., Sivonen, K., Börner, T. 1999 Nonribosomal peptide synthesis and toxigenicity of Cyanobacteria. J. Bacteriol., 181, 4089-4097.
Strain(s): 19248199102107204 
PubMed: 10383979

Li, R., Yokota, A., Sugiyama, J., Watanabe, M., Hiroki, M., Watanabe, M. M. 1998 Chemotaxonomy of planktonic cyanobacteria based on non-polar and 3-hydroxy fatty acid composition. Phycol. Res., 46, 21-28.
Keywords: 3-hydroxy fatty acids; chemotaxonomy; fatty acid; non-polar fatty acids; planktonic cyanobacteria
Strain(s): 2740447678798081878998102104111112204205207298604810821825826 
DOI: 10.1111/j.1440-1835.1998.tb00093.x

Neilan, B. A., Jacobs, D., Goodman, A. E. 1995 Genetic diversity and phylogeny of toxic cyanobacteria determined by DNA polymorphisms within the phycocyanin locus. Appl. Environ. Microbiol., 61, 3875-3883.
Strain(s): 244041447376788081878998102107111112204207298 
PubMed: 8526499

Soma, Y., Imaizumi, T., Yagi, K., Kasuga, S. 1993 Estimation of algal succession in lake water using HPLC analysis of pigments. Can. J. Fish. Aquat. Sci., 50, 1142-1146.
Strain(s): 44738188104111233234333351 

Watanabe, M. M., Kasai, F., Hiwatari, T., Suda, S., Nei, T. 1984 Cryopreservation of microalgae in liquid nitrogen - Survival of algal cultures after freezing. Jpn. J. Freez. Dry., 30, 23-26 (in Japanese with English title).
Strain(s): 1819212426293139404144454650737475767780818788899091939598991001011201221311351371441512042072132572633794152219223522392579 

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