Strain number NIES-25  
Phylum Cyanophyta  
Class Cyanophyceae  
Scientific name Nostoc linckia Bornet ex Bornet & Flauhault  
Synonym  
Former name  
Common name Blue-green alga ; Cyanobacteria  
Locality (Date of collection) Kagoshima Kagoshima Japan  
Latitude / Longitude  
Habitat (Isolation source)  
History < IAM (1983);  
Isolator (Date of isolation) Ishikawa, Masako  
Identified by Watanabe, Makoto M.  
State of strain Cryopreservation; Unialgal; Clonal; Axenic[2018 Feb]  
Culture condition
(Preculture condition)
Medium:  MDM (agar)  
Temperature:  20 (25) C
Light intensity:  1 (14-16) µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  4 M (14 D)  
Gene information Whole-genome ( AP018222 ) , 16S rRNA ( AB074503 ) , 16S-ITS-23S ( JN646757 JN646758 JN646759 ) , gyrB ( AB074769 ) , rpoC1 ( AB074792 ) , rpoD1 ( AB074819 )  
Cell size (min - max) 5 - 7 μm  
Organization Filamentous 
Characteristics Genome decoded strain  
Other strain no. Other collection strain no. : IAM M-16 (=M-251)
 
Remarks Cryopreserved; Axenic; Very slow growth 
Movie  
Reference
Curren, E., Teruaki, Y., Kuwahara, V. S., Leong, S. C. Y. 2019 Rapid profiling of tropical marine cyanobacterial communities. Reg. Stud. Mar. Sci., 25, 100485 (article ID).
Keywords: Cyanobacteria; MinION-Oxford Nanopore sequencer; Biodiversity; 16S rRNA amplicon sequencing; Long-read sequencing; Coastal waters; Southeast Asia; Monitoring
Strain(s): 22254284321352481 
DOI: 10.1016/j.rsma.2018.100485

Seo, P-S., Yokota, A. 2003 The phylogenetic relationships of cyanobacteria inferred from 16S rRNA, gyrB, rpoC1 and rpoD1 gene sequences. J. Gen. Appl. Micobiol., 49, 191-203.
Keywords: cyanobacteria; gyrB; phylogeny; protein evolution; rpoC1; rpoD1; 16S rDNA
Strain(s): 19212225391042093209521283277 
DOI: 10.2323/jgam.49.191

Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55.
Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection
Strain(s): 19212223242526272829303132333435363738394041444546505152535455565758596061626364656667686970737475767778798081878990919293949596979899100101102103104105106107108109110111112119120122123124125127128129130131132133134135137138139144147148150151152153154155156157158159160162163164165166167168169170171172173174175176177178179180181182183185186187188189191192193194195197198199200201202203204205206207208209210211212213214215216217224226227228229230231232241242243244245246248257259261262263266267268271287288289290294295297298299300301302303305306308309310311312313329334336337338339340341342349351359360361362375378379380382384385390394396397398415416418419421422423424425426427428429430431432433434436437438439440446447448449450451452453454455456457458459460464468469474478479480481503504505506507509510512514515522523524527528529530531532536537538539540541542543544545546564565566567568569570571572573574575576577578579580581582583584585592593594595596597598604610628630631632633634635636637638639640641642643644645646647648649650651652653654655656657658659660661662663664665666667672685686687688689690691692693694717718719720721722723724725726727728729 

Watanabe, A. 1960 List of algal strains in collection at the Institute of Applied Microbiology, University of Tokyo. J. Gen. Appl. Microbiol., 6, 283-292.
Strain(s): 192122232425262829374749226227 

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