The Microbial Culture Collection at the National Institute for Environmental Studies

Strain Data

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Strain number NIES-1332  
Phylum Rhodophyta   Class Cyanidiophyceae 
Scientific name Cyanidioschyzon merolae De Luca, Taddei et Varano  
Synonym  
Former name  
Common name Red alga  
Locality (Date of collection) Sardinia Isl. Italy  
Habitat (Isolation source) Hot spring (Water)  
History < Kuroiwa, Tsuneyoshi  
Isolator (Date of isolation) Toda, Kyoko  
Identified by  
State of strain Unialgal; Clonal; Non-axenic  
Preservation state
(State of preservation)
Subculture  
Culture condition
( Preculture condition )
Medium:  M-Allen  
Temperature: 37 C
Light intensity: 15-25 µmol photons/m2/sec     L/D cycle: 12L:12D
Duration: 2 M  
Gene information Mitochondrial DNA ( D89861 ) , Nuclear DNA (chromosome 1-20) ( AP006483-AP006502 ) , Plastid DNA ( AB002583 ) , aladh ( AB159599 ) , atpC ( AB159600 ) , gnd ( AB159595 AB159596 ) , Hsp70 ( AB095185 ) , Hsp90 ( AB095179 AB095186 ) , pgk ( AB159598 ) , psbO ( AB159597 ) , RPB1 ( AB095180 AB095187 ) , RpS8b ( AB095181 AB095188 ) , TPI ( AB095182 AB095189 ) , VatA ( AB095183 AB095190 ) , VatB ( AB095184 AB095191 )
Cell size (min - max) - 4 μm   Organization Unicellular; Non-flagellate 
Characteristics Autotrophic; Planktonic Acidophilic; Thermophilic  
Other strain no. Other strain no. : 10D  
Remarks Fragile species to temperature changes 

Reference
74)   Cunningham, Jr., F. X., Lee, H., Gantt, E. 2007 Carotenoid biosynthesis in the primitive red alga Cyanidioschyzon merolae. Eukaryot. Cell, 6, 533-545. , Strain(s): 1332 

166)   Hamana, K., Niitsu, M. 2006 Cellular polyamines of lower eukaryotes belonging to the phyla Glaucophyta, Rhodophyta, Cryptophyta, Haptophyta and Percolozoa. J. Gen. Appl. Microbiol., 52, 235-240. , Keywords: Cryptophyta; Glaucophyta; Haptophyta; Perclozoa; polyamine; Rhodophyta , Strain(s): 82742762772823533886997067157417637641016101710321036133214411456145714621463146714731516 

569)   Matsuzaki, M., Misumi, O., Shin-i, T., Maruyama, S., Takahara, M., Miyagishima, S., Mori, T., Nishida, K., Yagisawa, F., Nishida, K., Yoshida, Y., Nishimura, Y., Nakao, S., Kobayashi, T., Momoyama, Y., Higashiyama, T., Minoda, A., Sano, M., Nomoto, H., Oishi, K., Hayashi, H., Ohta, F., Nishizaka, S., Haga, S., Miura, S., Morishita, T., Kabeya, Y., Terasawa, K., Suzuki, Y., Ishii, Y., Asakawa, S., Takano, H., Ohta, N., Kuroiwa, H., Tanaka, K., Shimizu, N., Sugano, S., Sato, N., Nozaki, H., Ogasawara, N., Kohara, Y., Kuroiwa, T. 2004 Genome sequence of the ultrasmall unicellular red alga Cyanidioschyzon merolae 10D. Nature, 428, 653-657. , Strain(s): 1332 

801)   Nozaki, H., Matsuzaki, M., Misumi, O., Kuroiwa, H., Hasegawa, M., Higashiyama, T., Shin-i, T., Kohara, Y., Ogasawara, N., Kuroiwa, T. 2004 Cyanobarterial genes transmitted to the nucleus before divergence of red algae in the Chromista. J. Mol. Evol., 59, 103-113. , Keywords: Chromista; cyanobacterial nuclear genes; phylogeny; plastids; primary endosymbiosis; secondary endosymbiosis , Strain(s): 1332 

802)   Nozaki, H., Matsuzaki, M., Misumi, O., Kuroiwa, H., Higashiyama, T., Kuroiwa, T. 2005 Phylogenetic implications of the CAD complex from the primitive red alga Cyanidioschyzon merolae (Cyanidiales, Rhodophyta). J. Phycol., 41, 652-657. , Keywords: CAD complex; evolution; phylogeny; red algae; pyrimidine biosynthetic cluster , Strain(s): 1332 

803)   Nozaki, H., Matsuzaki, M., Takahara, M., Misumi, O., Kuroiwa, H., Hasegawa, M., Shin-i, T., Kohara, Y., Ogasawara, N., Kuroiwa, T. 2003 The phylogenetic position of red algae revealed by multiple nuclear genes from mitochondria-containing eukaryotes and an alternative hypothesis on the origin of plastids. J. Mol. Evol., 56, 485-497. , Keywords: eukaryotes; evolution; nuclear genes; outgroup; phylogeny; plantae; plastid; protein-coding genes; red algae , Strain(s): 1332 

815)   Nozaki, H., Takano, H., Misumi, O., Terasawa, K., Matsuzaki, M., Maruyama, S., Nishida, K., Yagisawa, F., Yoshida, Y., Fujiwara, T., Takio, S., Tamura, K., Chung, S. J., Nakamura, S., Kuroiwa, H., Tanaka, K., Sato, N., Kuroiwa, T. 2007 A 100%-complete sequence reveals unusually simple genomic features in the hot-spring red alga Cyanidioschyzon merolae. BMC Biol., 5, 28 (article ID). , Strain(s): 1332 

863)   Ohnuma, M., Kuroiwa, T., Tanaka, K. 2011 Optimization of cryopreservation conditions for the unicellular red alga Cyanidioschyzon merolae. J. Gen. Appl. Micobiol.,, 57, 137-143. , Keywords: cryoinjury; cryopreservation; Cyanidioschyzon merolae; unicellular red alga , Strain(s): 1332 

864)   Ohta, N., Matsuzaki, M., Misumi, O., Miyagishima, S., Nozaki, H., Tanaka, K., Shin-I, T., Kohara, Y., Kuroiwa, T. 2003 Complete sequence and analysis of the plastid genome of the unicellular red alga Cyanidioschyzon merolae. DNA Res., 10, 67-77. , Keywords: Cyanidioschyzon merolae; red algae; plastid; genome sequencing , Strain(s): 1332 

866)   Ohta, N., Sato, N., Kuroiwa, T. 1998 Structure and organization of the mitochondrial genome of the unicellular red alga Cyanidioschyzon merolae deduced from the complete nucleotide sequence. Nuc. Acid Res., 26, 5190-5198. , Strain(s): 1332