The Microbial Culture Collection at the National Institute for Environmental Studies

Strain Data


Strain number NIES-145  
Phylum Heterokontophyta   Class Raphidophyceae 
Scientific name Heterosigma akashiwo (Hada) Hada ex Y.Hara et Chihara  
Synonym -----  
Former name  
Common name  
Locality (Date of collection) Kagoshima Japan (1978-05-21)  
Habitat (Isolation source) Marine  
History < KAGAWA  
Isolator (Date of isolation) Yoshimatsu, Sadaaki (-----)  
Identified by Yoshimatsu, Sadaaki  
State of strain Unialgal; Clonal; Axenic[2013 Jan]  
Preservation state
(State of preservation)
Subculture  
Culture condition
( Preculture condition )
Medium:  f/2  
Temperature: 20 C
Light intensity: 32-40 µmol photons/m2/sec     L/D cycle: 12L:12D
Duration: 1 M  
Gene information 28S rRNA ( AB217645 ) , CO1 ( AB334510 ) , rbcL ( AB334437 )
Cell size (min - max)   Organization Unicellular; Flagellate 
Characteristics Red tide  
Other strain no. Other strain no. : KGW-11-5  
Remarks Fragile species to transportation stresses 

Reference
78)   Demura, M., Noël, M.-H., Kasai, F., Watanabe, M. M., Kawachi, M. 2009 Taxonomic revision of Chattonella antiqua, C. marina and C. ovata (Raphidophyceae) based on their morphological characteristics and genetic diversity. Phycologia, 48, 518-535. , Keywords: Chattonella antiqua; Chattonella marina; Chattonella ovata; COI; COX1; Genetic diversity; ITS; Microsatellite; Morphology; Phylogeny; Population; Raphidophyceae; rbcL; Speciation; Taxonomy , Strain(s): 12356910148384858611311411511611812114514616129355755855956160367171684918721873 

377)   Kamikawa, R., Asai, J., Miyahara, T., Murata, K., Oyama, K., Yoshimatsu, S., Yoshida, T., Sako, Y. 2006 Application of a real-time PCR assay to a comprehensive method of monitoring harmful algae. Mircobes Environ., 21, 163-173. , Keywords: harmful algal bloom (HAB); molecular monitoring; real-time PCR; red tide , Strain(s): 7121145603 

1239)   Uye, S., Takamatsu, K. 1990 Feeding inter-actions between planktonic copepods and red-tide flagellates from Japanese coastal waters. Mar. Ecol. Prog. Ser., 59, 97-107. , Strain(s): 1215136145221235251319