The Microbial Culture Collection at the National Institute for Environmental Studies

Strain Data

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Strain number NIES-1090  
Phylum Cyanophyta   Class Cyanophyceae 
Scientific name Microcystis aeruginosa (Kützing) Lemmermann  
Synonym Microcystis ichthyoblabe Kützing; Microcystis novacekii (Komárek) Compère; Microcystis viridis (A.Brown) Lemmermann; Microcystis wesenbergii Komárek  
Former name Microcystis ichthyoblabe Kützing  
Common name Blue-green alga  
Locality (Date of collection) Lake Barato Sapporo Hokkaido Japan (1984-08-24)  
Habitat (Isolation source) Freshwater (Lake water)  
History < TAC;  
Isolator (Date of isolation) Watanabe, Masayuki (1984-09-06)  
Identified by Watanabe, Masayuki  
State of strain Unialgal; Clonal; Non-axenic  
Preservation state
(State of preservation)
Cryopreservation  
Culture condition
( Preculture condition )
Medium:  MA  
Temperature: 18 C
Light intensity: 15-25 µmol photons/m2/sec     L/D cycle: 10L:14D
Duration: 1 M  
Gene information 16S rRNA ( AB012339 AB015367 AB023282 )
Cell size (min - max) - 5 μm   Organization  
Characteristics Cyanobacterial water bloom (aoko); Microcystin not detected Reisolated from TAC 91; ST 2 (Tanabe et al. 2007)  
Other strain no. Other strain no. : TAC 91-1  
Remarks Cryopreserved 

Reference
418)   Kato, T., Watanabe, M. F., Watanabe M. 1991 Allozyme divergence in Microcystis (Cyanophyceae) and its taxonomic inference. Algological Studies, 64, 129-140. , Keywords: cyanophytes/cyanobacteria; Microcystis; allozyme divergence; allozyme genotype; allozyme study; cell size; molecular taxonomy , Strain(s): 105510581061106310641067106810701071107410771079108110841086109010911093109510971098 

427)   Kawahara, K., Sagehashi, M., Fujii, T., Fujita, H., Hu, H.-Y., Sakoda, A. 2011 Potential of a green alga Botryococcus braunii for simultaneous water purification and biofuel production under open-air condition. J. Water Environ. Technol., 9, 29-37. , Keywords: biofuel production; Botryococcus braunii; Microcystis aeruginosa; water purification , Strain(s): 878361090 

489)   Kondo, R., Kagiya, G., Hiroishi, S., Watanabe, M. 2000 Genetic typing of a bloom-forming cyanobacterial genus Microcystis in Japan using 16S rRNA gene sequence analysis. Plankton Biol. Ecol., 47, 1-6. , Keywords: genetic typing; 16S rDNA; Microcystis; cyanobacteria; phylogenetic analysis , Strain(s): 878889909198991001011021041051061081091101111122982996041055106110681076109010911105112211421164 

490)   Kondo, R., Kagiya, G., Hiroishi, S., Watanabe, M., Hata, Y. 1999 Rapid DNA extraction from a bloom-forming cyanobacterium. Microb. Environ., 14, 157-161. , Keywords: DNA extraction; PCR; Microcystis; cyanobacteria , Strain(s): 878889909198991001011021041051061081091101111122982996041055106110681076109010911105112211421164 

491)   Kondo, R., Kagiya, G., Komura, M., Yuki, Y., Hiroishi, S., Watanabe, M. 2000 Molecular taxonomy of a bloom-forming cyanobacterial genus Microcystis. Bull. Plankton Soc. Japan, 47, 25-33 (in Japanese with English summary). , Keywords: cyanobacteria; Microcystis; bacterial criteria; molecular analysis , Strain(s): 6878889909198991001011021041051061081091101111122982996041055106110681076109010911105112211421164 

754)   Nishihara, H., Miwa, H., Watanabe, M., Nagashima, M., Yagi, O., Takamura, Y. 1997 Random amplified polymorphic DNA (RAPD) analyses for discriminating genotypes of Microcystis cyanobacteria. Biosci. Biotech. Biochem., 61, 1067-1072. , Keywords: RAPD analysis; DNA polymorphism; Microcystis; cyanobacterium; allozyme genotype , Strain(s): 106110671068107610771090109111051122113311421164 

898)   Onodera, H., Oshima, Y., Watanabe, M. F., Watanabe, M., Bolch, C. J., Blackburn, S., Yasumoto, T. 1996 Screening of paralytic shellfish toxins in freshwater cyanobacteria and chemical confirmation of the toxins in cultured Anabaena circinalis from Australia. In Intergovernmental Oceanographic Commissions of UNESCO, Eds. by Yasumoto, T., Oshima, Y. & Fukuyo, Y., p. 563-566. , Strain(s): 1063106810791084109010931099 

918)   Otsuka, S., Suda, S., Li, R., Watanabe, M., Oyaizu, H., Matsumoto, S., Watanabe, M. M. 1998 16S rDNA sequences and phylogenetic analyses of Microcystis strains with and without phycoerythrin. FEMS Mircobiol. Lett., 164, 119-124. , Keywords: cyanobacterium; Microcystis; 16S rDNA; taxonomy , Strain(s): 1054105510671072108510901143 

919)   Otsuka, S., Suda, S., Li, R., Watanabe, M., Oyaizu, H., Matsumoto, S., Watanabe, M. M. 1999 Phylogenetic relationships between toxic and non-toxic strains of the genus Microcystis based on 16S to 23S internal transcribed spacer sequence. FEMS Mircobiol. Lett., 172, 15-21. , Keywords: cyanobacteria; Microcystis; internal transcribed spacer; phylogeny; microcystin , Strain(s): 44104111105410551058106710681072108510861090109110921105111511171143 

926)   Park, H-D., Hayashi, H. 1994 Microcystis and microcystins [Microcystis zoku to mikuroshisuchin]. In Waterbloom of Blue-green Algae and Their Toxins [Aoko, sono shutsugen to dokuso], Eds. by Watanabe, M. F., Harada,K. & Fujii, H., University of Tokyo Press, p. 75-99 (in Japanese without English title). , Strain(s): 737410531054105510591061106310641066106710681070107110721074107610771079108010811083108410851086109010911093109510971098109911401154 

927)   Park, H-D., Watanabe, M. F. 1996 Toxic Microcystis in eutrophic lakes. In Toxic Microcystis, Eds. by Watanabe, M.F., Harada, K., Carmichael W.W. & Fujii, H., CRC Press, Boca Raton, p. 57-77. , Strain(s): 73741063107410791085108610901154 

928)   Park, H-D., Watanabe, M. F., Harada, K., Nagai, H., Suzuki, M., Watanabe, M., Hayashi, H. 1993 Hepatotoxin (microcystin) and neurotoxin (anatoxin-a) contained in natural blooms and strains of cyanobacteria from Japanese freshwaters. Natural Toxins, 1, 353-360. , Keywords: Anabaena; anatoxin-a; neurotoxin; Microcystis; microcystin; hepatotoxin; natural bloom; strain; Japanese freshwaters; simultaneous analysis of anatoxin-a and microcystin , Strain(s): 7374106310741079108510861090 

1171)   Tanabe, Y., Kasai, F., Watanabe, M. M. 2007 Multilocus sequence typing (MLST) reveals high genetic diversity and clonal population structure of the toxic cyanobacterium Microcystis aeruginosa. Microbiology, 153, 3695-3703. , Strain(s): 44878889909198991001011021031041051061071081091101111122982994786048439019331050105110521053105410551056105710581059106010611062106310641065106610671068106910701072107510761077108510901091109210931094109510961097109811001101110211031104110511061107110811131114111511221123112411251126112711281129113011311132113311341135113611371138113911401141114211431144114511461147114811491150115111521153115511561157115911601161116211631164117011711172120912111213121412151216121712181219122012211222122312241225122612271228122912301231123212331234123512361237123812391240124112421243124412451251125212531254 

1174)   Tanabe, Y., Sano, T., Kasai, F., Watanabe, M. M. 2009b Recombination, cryptic clades and neutral molecular divergence of the microcystin synthetase (mcy) genes of toxic cyanobacterium Microcystis aeruginosa. BMC Evol. Biol., 9, 115 (article ID). , Strain(s): 44878889909198991001011021031041051061071081091101111122982994786048439011050105110521053105410551056105710581059106010611062106310641065106610671068106910701072107510761077108510901091109210931094109510961097109811001101110211031104110511061107110811131114111511221123112411251126112711281129113011311132113311341135113611371138113911401141114211431144114511461147114811491150115111521153115511561157115911601161116211631164117011711172120912111213121412151216121712181219122012211222122312241225122612271228122912301231123212331234123512361237123812391240124112421243124412451251125212531254246524662467246924702471247224732474247524762477247824792561 

1175)   Tanabe, Y., Watanabe, M. M. 2011 Local expansion of a panmictic lineage of water bloom-forming cyanobacterium Microcystis aeruginosa. PLos ONE, 6, e17085 (article ID). , Strain(s): 448788899091989910010110210310410510610710810911011111229829947860484390110501051105210531054105510561057105810591060106110621063106410651066106710681069107010721075107610771085109010911092109310941095109610971098110011011102110311041105110611071108111311141115112211231124112511261127112811291130113111321133113411351136113711381139114011411142114311441145114611471148114911501151115211531155115611571159116011611162116311641170117111721173117411751176117711791180118111831184118511861209121112131214121512161217121812191220122112221223122412251226122712281229123012311232123312341235123612371238123912401241124212431244124512511252125312542088208920902091246524662467246824692470247124722473247424752476247724782479248024812482248324842485248624872488248924902491249224932494249524962546254725482549255025512552255325542555255625572558255925602561259125922593259425952596259725982599260026012602260326042605260626072608260926102611261226132617261826192620262126222623262426252626262726282629 

1264)   Watanabe, M. 1996 Isolation, cultivation and classification of bloom-forming Microcystis in Japan. In Toxic Microcystis, Eds. by Watanabe, M.F., Harada, K., Carmichael W.W. & Fujii, H., CRC Press, Boca Raton, p. 13-34. , Strain(s): 105010511052105310541061106310641065106610671068107010711072107410751076107710791080108110821083108510861087108810891090109310951097109810991105110711081109111011131115111611171122112411251126112711301132113311341139114011411142114611471149115211531154115511571159116011611162116311641173117811821183118411871189119411961198120012031205120612091213121612191222122512301232123412371239124112431246 

1267)   Watanabe, M. F. 1996 Production of microcystins. In Toxic Microcystis, Eds. by Watanabe, M. F., Harada, K., Carmichael W. W. & Fujii, H., CRC Press, Boca Raton, p. 35-56. , Strain(s): 1053105410591061106310641066106710681070107110721074107610771079108110831085108610901091109310951097109810991140 

1270)   Watanabe, M. F., Watanabe, M., Kato, T., Harada, K., Suzuki, M. 1991 Composition of cyclic peptide toxins among strains of Microcystis aeruginosa (blue-green algae, Cyanobacteria). Bot. Mag. Tokyo, 104, 49-57. , Keywords: blue-green algae; cyanobacteria; cyclic peptide; Microcystis; strain; toxin , Strain(s): 10531054106010611063106410661067106810701071107210741076107710791080108110831085108610901093109510971098109911401142