Strain number NIES-1000  
Phylum Haptophyta  
Class Prymnesiophyceae  
Scientific name Gephyrocapsa oceanica Kamptner  
Synonym Coccolithus oceanicus (Kamptner) Kamptner 1955; Crenalithus doronicoides P.H.Roth 1973  
Former name  
Common name Coccolithophore  
Locality (Date of collection) Chotaro-ike Pond, Miyake Isl. Miyake Vil. Tokyo Japan (1999-11-23)  
Latitude / Longitude 34.053641 / 139.538773 
Habitat (Isolation source) Marine (Seawater)  
History < Kawachi, Masanobu  
Isolator (Date of isolation) Kawachi, Masanobu (1999-11-25)  
Identified by Kawachi, Masanobu  
State of strain Subculture; Unialgal; Clonal; Non-axenic; Coccolith(+/-)[2014 Nov]  
Culture condition
(Preculture condition)
Medium:  MNK  
Temperature:  20 C
Light intensity:  19 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  21 D  
Gene information 28S rRNA ( LC102345 ) , CO1b, atp4 ( AB563742 ) , CO3 ( JN098174 ) , CO1 ( GQ246010 )  
Cell size (min - max) - 10 μm  
Organization Unicellular 
Characteristics Red tide ; Planktonic  
Other strain no. Other collection strain no. : CCMP 2054; RCC 1562
 
Remarks  
Movie  
Reference
Araie, H., Nakamura, H., Toney, J. L., Haigg, H. A., Plancq, J., Shiratori, T., Leavitt, P. R., Seki, O., Ishida, K-I., Sawada, K., Suzuki, I., Shiraiwa, Y. 2018 Novel alkenone-producing strains of genus Isochrysis (Haptophyta) isolated from Canadian saline lakes show temperature sensitivity of alkenones and alkenoates. Org. Geochem., 121, 89-103.
Keywords: Alkenones; Alkenone unsaturation index; Canadian salt lakes; Chemotaxonomy; Haptophytes; Isochrysis; Long-chain alkyl ketones; Paleothermometer; UK37
Strain(s): 10002697 
DOI: 10.1016/j.orggeochem.2018.04.008

Nakamura, H., Sawada, K., Araie, H., Shiratori, T., Ishida, K-I., Suzuki, I., Shiraiwa, Y. 2016 Composition of long chain alkenones and alkenoates as a function of growth temperature in marine haptophyte Tisochrysis lutea. Org. Geochem., 99, 78-89.
Keywords: Alkenones; Chemotaxonomy; Tisochrysis; Isochrysis; UK37
Strain(s): 100025902697 
DOI: 10.1016/j.orggeochem.2016.06.006

Bendif, E. M., Probert, I., Carmichael, M., Romac, S., Hagino, K., de Vardas, C. 2014 Genetic delineation between and within the widespread coccolithophore morpho-species Emiliania huzleyi and Gephyrocapsa. J. Phycol., 50, 140-148.
Keywords: coccolithophore; DNA barcoding; Emiliania huxleyi; Gephyrocapsa oceanica; phylogeny; species complex
Strain(s): 10002697 
PubMed: 26988015
DOI: 10.1111/jpy.12147

del Campo, J., Sieracki, M.E., Molestina, R., Keeling, P., Massana, R., Ruiz-Trillo, I. 2014 The others: our biased perspective of eukaryotic genomes. Trends Ecol. Evol., 29, 252-259.
Strain(s): 100025822584258725902670267326912693269426952696269726992700275127532755275627582761336933703371 
PubMed: 24726347
DOI: 10.1016/j.tree.2014.03.006

Hagino, K., Bendif, E. M., Young, J. R. Kogame, K., Probert, I., Takano, Y., Horiguchi, T., de Vardas, C., Okada, H. 2011 New evidence for morphological and genetic variation in the cosmopolitan coccolithophore Emiliania huxleyi (Prymnesiophyceae) from the COX1b-ATP4 genes. J. Phycol., 47, 1164-1176.
Keywords: biogeography; coccolithophore; Emiliania huxleyi; mitochondrial DNA; morphotype
Strain(s): 83783810001311131213131314 
DOI: 10.1111/j.1529-8817.2011.01053.x

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