Strain number NIES-991  
Phylum Cyanophyta  
Class Cyanophyceae  
Scientific name Raphidiopsis raciborskii (Woloszynska) Aguilera, Berrendero Gómez, Kastovsky, Echenique & Salerno  
Synonym Anabaena raciborskii Woloszynska; Cylindrospermopsis raciborskii (Woloszynska) Seenayya & Subba Raju 1972  
Former name Formerly identified as Cylindrospermopsis raciborskii, re-named based on Aguilera et al. 2018[2018 Apr]  
Common name Blue-green alga ; Cyanobacteria  
Locality (Date of collection) Gonoike Pond Kamisu Ibaraki Japan (2001-07-**)  
Latitude / Longitude 35.889908 / 140.670036 
Habitat (Isolation source) Freshwater (Pond water)  
History < Yongmanitchai, W.  
Isolator (Date of isolation) Chonudomkul, D. (2001-07-**)  
Identified by Yongmanitchai, W.  
State of strain Subculture; Unialgal; Clonal; Axenic[2018 Jan]  
Culture condition
(Preculture condition)
Medium:  CT  
Temperature:  20 C
Light intensity:  18-20 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  1 M  
Gene information 16S rRNA ( AB115487 ) , 16S-ITS-23S ( AB638219 )  
Cell size (min - max)  
Organization Filamentous 
Characteristics Cyanobacterial water bloom (aoko) ; Non-toxic ; Planktonic  
Other strain no. Other strain no. : KJA1  
Remarks Fragile species to transportation stresses; Read and agree "How to order 4.1"; Axenic 
Movie  
Reference
Hodoki, Y., Ohbayashi, K., Kobayashi, Y., Okuda, N., Nakano, S. 2012 Detection and identification of potentially toxic cyanobacteria: Ubiquitous distribution of Microcystis aeruginosa and Cuspidothrix issatschenkoi in Japanese lakes. Harmful Algae, 16, 49-57.
Keywords: Anatoxin-a; Automated rRNA intergenic spacer analysis (ARISA); Cuspidothrix issatschenkoi; Cyanotoxin; Microcystin; Microcystis aeruginosa
Strain(s): 304077102111230512843905928932937991164516691698172919502116 
DOI: 10.1016/j.hal.2012.01.003

Chonudomkul, D., Yongmanitchai, W., Theeragool, G., Kawachi, M., Kasai, F., Kaya, K., Watanabe, M. M. 2004 Morphology, genetic diversity, temperature tolerance and toxicity of Cylindrospermopsis raciborskii (Nostocales, Cyanobacteria) strains from Thailand and Japan. FEMS Microbiol. Ecol., 48, 345-355.
Keywords: toxic cyanobacteria; Cylindrospermopsis raciborskii; STRR sequences; Thailand; Japan; low-temperature adapted group; genotypic clusters
Strain(s): 99199299310401041 
PubMed: 19712304
DOI: 10.1016/j.femsec.2004.02.014

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