The Microbial Culture Collection at the National Institute for Environmental Studies

Strain Data


Strain number NIES-125  
Phylum Streptophyta   Class Charophyceae 
Scientific name Closterium acerosum Ehrenberg ex Ralfs  
Synonym -----  
Former name  
Common name Desmid  
Locality (Date of collection) Rukumkot Nepal (1965-10-29)  
Habitat (Isolation source) Freshwater (Pond sediment)  
History < Watanabe, Masayuki  
Isolator (Date of isolation) Ichimura, Terunobu (-----)  
Identified by Ichimura, Terunobu  
State of strain Unialgal; Clonal; Axenic[2012 Dec]  
Preservation state
(State of preservation)
Subculture  
Culture condition
( Preculture condition )
Medium:  C  
Temperature: 20(20) C
Light intensity: 8-15(15-27) µmol photons/m2/sec     L/D cycle: 12L:12D
Duration: 3 M(1 M)  
Gene information 18S rRNA ( AF352230 )
Cell size (min - max)   Organization Unicellular; Non-flagellate 
Characteristics  
Other strain no. Other strain no. : N-25-22  
Remarks  

Reference
79)   Denboh, T., Hendrayanti, D., Ichimura, T. 2001 Monophyly of the genus Closterium and the order Desmidiales (Charophyceae, Chlorophyta) inferred from nuclear small subunit rDNA data. J. Phycol., 37, 1063-1072. , Keywords: Charophyceae; Closterium; desmid; molecular phylogeny; placoderm; SSU rDNA; Zygnematales , Strain(s): 52125165170174175180181185187188191194198199200271339449 

145)   Gontcharov, A. A., Marin, B., Melkonian, M. 2003 Molecular phylogeny of conjugating green algae (Zygnemophyceae, Streptophyta) inferred from SSU rDNA sequence comparisons. J. Mol. Evol., 56, 89-104. , Keywords: Green algae; Streptophyta; Zygnemophyceae; Desmids; Molecular phylogeny; SSU rDNA , Strain(s): 125309 

267)   Ichimura, T. 1973 The life cycle and its control in some species of Closterium, with special reference to the biological species problems. Thesis D. Sci., University of Tokyo, 69 pp., 11 tables, 40 figs. , Strain(s): 666768124125127174175176177179180181182183185198202262337338450451 

635)   Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55. , Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection , Strain(s): 19212223242526272829303132333435363738394041444546505152535455565758596061626364656667686970737475767778798081878990919293949596979899100101102103104105106107108109110111112119120122123124125127128129130131132133134135137138139144147148150151152153154155156157158159160162163164165166167168169170171172173174175176177178179180181182183185186187188189191192193194195196197198199200201202203204205206207208209210211212213214215216217224226227228229230231232241242243244245246248257259261262263266267268271287288289290294295297298299300301302303305306308309310311312313329334336337338339340341342349351359360361362375378379380382384385390394396397398415416418419421422423424425426427428429430431432433434436437438439440446447448449450451452453454455456457458459460464468469474478479480481503504505506507509510512514515522523524527528529530531532536537538539540541542543544545546564565566567568569570571572573574575576577578579580581582583584585592593594595596597598604610628630631632633634635636637638639640641642643644645646647648649650651652653654655656657658659660661662663664665666667672685686687688689690691692693694717718719720721722723724725726727728729