The Microbial Culture Collection at the National Institute for Environmental Studies

Strain Data

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Strain number NIES-124  
Phylum Streptophyta   Class Charophyceae 
Scientific name Closterium acerosum Ehrenberg ex Ralfs  
Synonym -----  
Former name  
Common name Desmid  
Locality (Date of collection) Daramshara Nepal (1965-10-25)  
Habitat (Isolation source) Freshwater (Pond sediment)  
History < Watanabe, Masayuki  
Isolator (Date of isolation) Ichimura, Terunobu (-----)  
Identified by Ichimura, Terunobu  
State of strain Unialgal; Clonal; Axenic[2013 Jan]  
Preservation state
(State of preservation)
Subculture  
Culture condition
( Preculture condition )
Medium:  C  
Temperature: 20(20) C
Light intensity: 8-15(15-27) µmol photons/m2/sec     L/D cycle: 12L:12D
Duration: 3 M(1 M)  
Gene information
Cell size (min - max) - 71 μm   Organization Unicellular; Non-flagellate 
Characteristics  
Other strain no. Other strain no. : N-20-1  
Remarks  

Reference
264)   Ichimura, T. 1973 The life cycle and its control in some species of Closterium, with special reference to the biological species problems. Thesis D. Sci., University of Tokyo, 69 pp., 11 tables, 40 figs. , Strain(s): 666768124125127174175176177179180181182183185198202262337338450451 

632)   Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55. , Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection , Strain(s): 19212223242526272829303132333435363738394041444546505152535455565758596061626364656667686970737475767778798081878990919293949596979899100101102103104105106107108109110111112119120122123124125127128129130131132133134135137138139144147148150151152153154155156157158159160162163164165166167168169170171172173174175176177178179180181182183185186187188189191192193194195196197198199200201202203204205206207208209210211212213214215216217224226227228229230231232241242243244245246248257259261262263266267268271287288289290294295297298299300301302303305306308309310311312313329334336337338339340341342349351359360361362375378379380382384385390394396397398415416418419421422423424425426427428429430431432433434436437438439440446447448449450451452453454455456457458459460464468469474478479480481503504505506507509510512514515522523524527528529530531532536537538539540541542543544545546564565566567568569570571572573574575576577578579580581582583584585592593594595596597598604610628630631632633634635636637638639640641642643644645646647648649650651652653654655656657658659660661662663664665666667672685686687688689690691692693694717718719720721722723724725726727728729