Strain number NIES-843  
Phylum Cyanophyta  
Class Cyanophyceae  
Scientific name Microcystis aeruginosa (Kützing) Lemmermann  
Synonym Microcystis ichthyoblabe Kützing; Microcystis novacekii (Komárek) Compère; Microcystis viridis (A.Brown) Lemmermann; Microcystis wesenbergii Komárek  
Former name  
Common name Blue-green alga ; Cyanobacteria  
Locality (Date of collection) Lake Kasumigaura Ibaraki Japan (1997-08-28)  
Latitude / Longitude 36.01333333 / 140.36638888 
Habitat (Isolation source) Freshwater (Lake water)  
History < Otsuka, Shigeto  
Isolator (Date of isolation) Otsuka, Shigeto (1997-08-28)  
Identified by Otsuka, Shigeto  
State of strain Cryopreservation; Unialgal; Clonal; Axenic  
Culture condition
(Preculture condition)
Medium:  MA  
Temperature:  25 C
Light intensity:  20-30 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  1 M  
Gene information Whole-genome ( AP009552 ) , 16S rRNA ( AB035549 ) , ftsZ ( AB324867 ) , glnA ( AB324946 ) , gltX ( AB325025 ) , gyrB ( AB325104 ) , pgi ( AB325183 ) , recA ( AB325262 ) , tpi ( AB325341 )  
Cell size (min - max)  
Characteristics Cyanobacterial water bloom (aoko) ; Offensive odor ; Toxicity ; Autotrophic ; Planktonic ; Type strain (Otsuka, S. et al. 2001) ; Genome decoded strain (Kaneko, T. et al. 2007) ; ST18 (Tanabe, Y. et al. 2007)  
Other strain no. Other collection strain no. : IAM M-247
Other strain no. : NC7  
Remarks Toxicity; Cryopreserved 
Imanishi, S. Y., Nakayama, T., Asukabe, H., Harada, K. 2017 Application of MALDI Biotyper to cyanobacterial profiling. Rapid Commun. Mass Spectrom., 31, 325-332.
Strain(s): 738910210310711229861180880983584398910251043105810711099164216521672 
PubMed: 27862451
DOI: 10.1002/rcm.7793

Xuan, H., Dai, X., Li, J., Zhang, X., Yang, C., Luo, F. 2017 A Bacillus sp. strain with antagonistic activity against Fusarium graminearum kills Microcystis aeruginosa selectively. Sci. Total Environ., 583, 214-221.
Keywords: Harmful algal blooms; Algicidal activity; Antagonism; Bifunctional biocontrol agent
Strain(s): 4490843 
PubMed: 28104332
DOI: 10.1016/j.scitotenv.2017.01.055

Lapsongpon, T., Leungprasert, S., Yoshimura, C. 2017 Pre-chlorination contact time and the removal and control of Microcystis aeroginosa in coagulation. 7th International Conference on Environment and Industrial Innovation, 012011 (article ID).
Strain(s): 843 
DOI: 10.1088/1755-1315/67/1/012011

Srivastava, A., Ko,S-R., Ahn, C-Y., Oh, H-M., Ravi, A. K., Asthana, R. K. 2016 Microcystin biosynthesis and mcyA expression in geographically distinct Microcystis strains under different nitrogen, phosphorus, and boron regimes. Biomed Res. Int., 2016, 5985987 (article ID).
Strain(s): 843 
DOI: 10.1155/2016/5985987

Shao, J., He, Y., Li, F., Zhang, H., Chen, A., Luo, S., Gu, J.-D. 2016 Growth inhibition and possible mechanism of oleamide against the toxin-producing cyanobacterium Microcystis aeruginosa NIES-843. Ecotoxicology, 25, 225-233.
Keywords: Oleamide; Microcystis aeruginosa; Growth inhibition; Photosynthesis; Microcystins
Strain(s): 843 
DOI: 10.1007/s10646-015-1582-x

Sun, L.W., Jiang, W.J., Sato, H., Kawachi, M., Lu, X.W. 2016 Rapid classification and identification of Microcystis aeruginosa strains using MALDI-TOF MS and polygenetic analysis. PLoS One, 11, e0156275 (article ID).
Strain(s): 44878889901001011041051071091111124786048439011043105310581061106810691070107210761077108510941099110111041105111311151126113411351144115111571170121112131215121612171218122212241230123212331239 
DOI: 10.1371/journal.pone.0156275

Meglič, A., Pecman, A., Rozina, T., Leštan, D., Sedmak, B. 2016 Electrochemical inactivation of cyanobacteria and microcystin degradation using a boron-doped diamond anode - A potential tool for cyanobacterial bloom control. J. Environ. Sci., 53, 248-261.
Keywords: Cyanobacterial bloom control; Boron-doped diamond anode; Electro-oxidation; Microcystin degradation; Protein phosphatase-1 inhibition; Water remediation
Strain(s): 843 
DOI: 10.1016/j.jes.2016.02.016

Manali, KM., Arunraj, R., Kumar, T., Ramya, M. 2016 Detection of microcystin producing cyanobacteria in Spirulina dietary supplements using multiplex HRM quantitative PCR. J. Appl. Phycol.,
Keywords: Microcystin; Arthrospira platensis; Multiplex HRM qPCR; Dietary supplements; Liquid; chromatography–high-resolution mass spectrometr
Strain(s): 843 
DOI: 10.1007/s10811-016-1011-4

Harke, M. J., Jankowiak, J. G., Morrell, B. K., Gobler, C. J. 2016 Transcriptomic responses in the bloom-forming cyanobacterium Microcystis 2 induced during exposure to zooplankton. Appl. Environ. Microbiol., 83, e02832-16 (article ID).
Keywords: microcystis; RNA-Seq; transcriptome; grazing
Strain(s): 843 
PubMed: 28003198
DOI: 10.1128/AEM.02832-16

Benke, P. I., Vinay Kumar, M. C. S., Pan, D., Swarup, S. 2015 A mass spectrometry-based unique fragment approach for the identification of microcystins. Analyst, 140, 1198-1206.
Strain(s): 843 
DOI: 10.1039/c4an01702a

Shao, J., Jiang, Y., Wang, Z., Peng, L., Luo, S., Gu, J., Li, R. 2014 Interactions between algicidal bacteria and the cyanobacterium Microcystis aeruginosa: lytic characteristics and physiological responses in the cyanobacteria. Int. J. Environ. Sci. Technol., 11, 469-476.
Keywords: Bacillus sp.; Gene expressions; Lytic characteristics; Microcystis aeruginosa; Oxidative stress; Photosynthesis inhibition
Strain(s): 843 
DOI: 10.1007/s13762-013-0205-4

Fukuda, S., Iwamoto, K., Atsumi, M., Yokoyama, A., Nakayama, T., Ishida, K-I., Inouye, I., Shiraiwa, Y. 2014 Global searches for microalgae and aquatic plants that can eliminate radioactive cesium, iodine and strontium from the radio-polluted aquatic environment: a bioremediation strategy. J. Plant Res., 127, 79-89.
Keywords: Algal phytoremediation; Bioaccumulation; Radiopollution; Radionuclide elimination; Radioactive cesium; The Fukushima 1 Nuclear Power Plant accident
Strain(s): 243671155160203233246329333391405417440487503529531538548553587597678727805843859931995100310121015101710311044104512591326133113771382138814111435143914401441144214581728182618311833184018461862186519562131214421462147215021752176218522682325234123522377241124122437285428552856285828592860 
DOI: 10.1016/10.1007/s10265-013-0596-9

Steffen, M. M., Dearth, S. P., Dill, B. D., Li, Z., Larsen, K. M., Campagna, S. R., Wilhelm, S. W. 2014 Nutrients drive transcriptional changes that maintain metabolic homeostasis but alter genome architecture in Microcystis. ISME Journal, 8, 2080-2092.
Keywords: cyanobacteria; metabolomics; nitrogen; transcriptomics; transposase; urea
Strain(s): 843 
PubMed: 24858783
DOI: 10.1038/ismej.2014.78

Shao, J., Peng, L., Luo, S., Yu, G., Gu, J.-D., Lin, S., Li, R. 2013 First report on the allelopathic effect of Tychonema bourrellyi (Cyanobacteria) against Microcystis aeruginosa (Cyanobacteria). J. Appl. Phycol., 25, 1567-1573.
Keywords: Tychonema bourrellyi; Microcystis aeruginosa; Allelopathy; Volatile substances; ß-Ionone; Cyanobacteria
Strain(s): 843 
DOI: 10.1007/s10811-012-9969-z

Shao, J., Liu, D., Gong, D., Zeng, Q., Yan, Z., Gu, J.-D. 2013 Inhibitory effects of sanguinarine against the cyanobacterium Microcystis aeruginosa NIES-843 and possible mechanisms of action. Aquat. Toxicol., 142-143, 257-263.
Keywords: Aldehydes; Chattonella marina; fatty acids; harmful phytoplankton; ichthyotoxicity; rainbow trout gill cell line; RT gill-W1
Strain(s): 843 

Shao, J., Wang, Z., Liu, Y., Liu, H., Peng, X., Wei, X., Lei, M., Li, R. 2012 Physiological responses of Microcystis aeruginosa NIES-843 (cyanobacterium) under the stress of chitosan modified kaolinite (CMK) loading. Ecotoxicology, 21, 698-704.
Keywords: Physiological responses; Microcystis aeruginosa; Chitosan modified kaolinite
Strain(s): 843 

Du, HW., Yang, LY., Wu, J., Xiao, L., Wang, XL., Jiang, LJ. 2012 Simultaneous removal of phosphorus and nitrogen in a sequencing batch biofilm reactor with transgenic bacteria expressing polyphosphate kinase. Appl. Microbiol. Biotechnol., 96, 265-272.
Keywords: Poly-phosphate kinase gene; Pseudomonas putida; Sequencing batch biofilm reactor; Phosphorus removal; Nitrogen removal
Strain(s): 843 
PubMed: 22218771
DOI: 10.1007/s00253-011-3839-5

Hodoki, Y., Ohbayashi, K., Kobayashi, Y., Okuda, N., Nakano, S. 2012 Detection and identification of potentially toxic cyanobacteria: ubiquitous distribution of Microcystis aeruginosa and Cuspidothrix issatschenkoi in Japanese lakes. Harmful Algae, 16, 49-57.
Keywords: Anatoxin-a; Automated rRNA intergenic spacer analysis (ARISA); Cuspidothrix issatschenkoi; Cyanotoxin; Microcystin; Microcystis aeruginosa
Strain(s): 304077102111230512843905928932937991164516691698172919502116 

Harke, M. J., Berry, D. L., Ammerman, J. W., Gobler, C. J. 2012 Molecular response of the bloom-forming cyanobacterium, Microcystis aeruginosa, to phosphorus Limitation. Microb. Ecol., 63, 188-198.
Strain(s): 843 
PubMed: 21720829
DOI: 10.1007/s00248-011-9894-8

Hasegawa, M., Nishizawa, A., Tsuji, K., Kimura, S., Harada, K. 2012 Volatile organic compounds derived from 2-keto-acid decarboxylase in Microcystis aeruginosa. Microbes. Environ., 27, 525-528.
Keywords: cyanobacteria; volatile organic compounds; 3-methyl-1-butanol; 2-keto-acid decarboxylase; Microcystis
Strain(s): 843 

Leikoski, N., Fewer, D. P., Jokela, J., Alakoski, P., Wahlsten, M., Sivonen, K. 2012 Analysis of an inactive cyanobactin biosynthetic gene cluster leads to discovery of new natural products from strains of the genus Microcystis. PLoS One, 7, e43002 (article ID).
Strain(s): 843 
DOI: 10.1371/journal.pone.0043002

Tanabe, Y., Watanabe, M. M. 2011 Local expansion of a panmictic lineage of water bloom-forming cyanobacterium Microcystis aeruginosa. PLoS One, 6, e17085 (article ID).
Strain(s): 448788899091989910010110210310410510610710810911011111229829947860484390110501051105210531054105510561057105810591060106110621063106410651066106710681069107010721075107610771085109010911092109310941095109610971098110011011102110311041105110611071108111311141115112211231124112511261127112811291130113111321133113411351136113711381139114011411142114311441145114611471148114911501151115211531155115611571159116011611162116311641170117111721173117411751176117711791180118111831184118511861209121112131214121512161217121812191220122112221223122412251226122712281229123012311232123312341235123612371238123912401241124212431244124512511252125312542088208920902091246524662467246824692470247124722473247424752476247724782479248024812482248324842485248624872488248924902491249224932494249524962546254725482549255025512552255325542555255625572558255925602561259125922593259425952596259725982599260026012602260326042605260626072608260926102611261226132617261826192620262126222623262426252626262726282629 

Shao, J., Xu, Y., Wang, Z., Jiang, Y., Yu, G., Peng, X., Li, R. 2011 Elucidating the toxicity targets of β-ionone on photosynthetic system of Microcystis aeruginosa NIES-843 (cyanobacteria). Aquat. Toxicol., 104, 48-55.
Keywords: Microcystis aeruginosa; Photosynthesis; Gene expression; Chlorophyll a fluorescence
Strain(s): 843 
PubMed: 21543049
DOI: 10.1016/j.aquatox.2011.03.014

Hodoki, Y., Ohbayashi, K., Kobayashi, Y., Okuda, N., Nakano, S.-I. 2011 Temporal variation in cyanobacteria species composition and photosynthetic activity in experimentally induced blooms. J. Plankton Res., 33, 1410-1416.
Keywords: cyanobacterial bloom; DCMU [3'-(3,4-dichlorophenyl)-1',1'-dimethyl urea]; maximum quantum yieldMicrocystis aeruginosa
Strain(s): 843 

Weiz, A. R., Ishida, K., Makower, K., Ziemert, N., Hertweck, C., Dittmann, E. 2011 Leader peptide and a membrane protein scaffold guide the biosynthesis of the tricyclic peptide microviridin. Chem. Biol., 18, 1413-1421.
Strain(s): 298843 
DOI: 10.1016/j.chembiol.2011.09.011

Kuno, S., Yoshida, T., Kamikawa, R., Hosoda, N., Sako, Y. 2010 The distribution of a phage-related insertion sequence element in the cyanobacterium, Microcystis aeruginosa. Microbes. Environ., 25, 295-301.
Keywords: cyanobacteria; Microcystis; cyanophage; insertion sequence; horizontal gene transfer
Strain(s): 90919899101102112298604843105810671070108610981105 

Shao, J., Yu, G, Wang, Z., Wu, Z, Peng, X., Li, R. 2010 Towards clarification of the inhibitory mechanism of wheat bran leachate on Microcystis aeruginosa NIES-843 (cyanobacteria): physiological responses. Ecotoxicology, 19, 1634-1641.
Keywords: Wheat bran leachate; Microcystis; Gene expression; Photosynthesis
Strain(s): 843 

Liu, SH., Zhang, PY., Cong, BL., Liu, CL., Lin XZ., Shen, JH., Huang, XH. 2010 Molecular cloning and expression analysis of a cytosolic Hsp70 gene from Antarctic ice algae Chlamydomonas sp. ICE-L. Extremophiles, 14, 329-337.
Keywords: Antarctic ice algae; Chlamydomonas sp. ICE-L; Heat shock protein; Hsp70; Psychrophile; Stress
Strain(s): 843 

Ziemert, N., Ishida, K., Weiz, A., Hertweck, C., Dittmann, E. 2010 Exploiting the natural diversity of microviridin gene clusters for discovery of novel tricyclic depsipeptides. Appl. Environ. Microbiol., 76, 3568-3574.
Strain(s): 100101102843 

Lee, Y.-K., Ahn, C.-Y., Kim, H.-S., Oh, H.-M. 2010 Cyanobactericidal effect of Rhodococcus sp. isolated from eutrophic lake on Microcystis sp. Biotechnol. Lett., 32, 1673-1678.
Keywords: Algicide; Bloom; Cyanobacteria; Microcystis; Rhodococcus
Strain(s): 23843 

Tanabe, Y., Sano, T., Kasai, F., Watanabe, M. M. 2009b Recombination, cryptic clades and neutral molecular divergence of the microcystin synthetase (mcy) genes of toxic cyanobacterium Microcystis aeruginosa. BMC Evol. Biol., 9, 115 (article ID).
Strain(s): 44878889909198991001011021031041051061071081091101111122982994786048439011050105110521053105410551056105710581059106010611062106310641065106610671068106910701072107510761077108510901091109210931094109510961097109811001101110211031104110511061107110811131114111511221123112411251126112711281129113011311132113311341135113611371138113911401141114211431144114511461147114811491150115111521153115511561157115911601161116211631164117011711172120912111213121412151216121712181219122012211222122312241225122612271228122912301231123212331234123512361237123812391240124112421243124412451251125212531254246524662467246924702471247224732474247524762477247824792561 
DOI: 10.1186/1471-2148-9-115

Ishida, K., Welker, M., Christiansen, G., Cadel-Six, S., Bouchier, C., Dittmann, E., Hertweck, C., de Marsac, N. T. 2009 Plasticity and evolution of aeruginosin biosynthesis in Cyanobacteria. Appl. Environ. Microbiol., 75, 2017-2026.
Strain(s): 98843 

Tomioka, N., Nagai, T., Kawasaki, T., Imai, A., Kohata, K. 2008 Quantification of Microcystis in a eutrophic lake by simple DNA extraction and SYBR green real-time PCR. Microbes. Environ., 23, 306-312.
Keywords: Microcystis; quantification; SYBR Green I; DNA extraction; eutrophic lake
Strain(s): 9899204604611843946961 

Kaneko, T., Nakajima, N., Okamoto, S., Suzuki, I., Tanabe, Y., Tamaoki, M., Nakamura, Y., Kasai, F., Watanabe, A., Kawashima, K., Kishida, Y., Ono, A., Shimizu, Y., Takahashi, C., Minami, C., Fujishiro, T., Kohara, M., Katoh, M., Nakazaki, N., Nakayama, S., Yamada, M., Tabata, S., Watanabe, M. M. 2007 Complete genome structure of the bloom-forming toxic cyanobacterium Microcystis aeruginosa NIES-843. DNA Res., 14, 247-256.
Strain(s): 843 

Tanabe, Y., Kasai, F., Watanabe, M. M. 2007 Multilocus sequence typing (MLST) reveals high genetic diversity and clonal population structure of the toxic cyanobacterium Microcystis aeruginosa. Microbiology, 153, 3695-3703.
Strain(s): 44878889909198991001011021031041051061071081091101111122982994786048439019331050105110521053105410551056105710581059106010611062106310641065106610671068106910701072107510761077108510901091109210931094109510961097109811001101110211031104110511061107110811131114111511221123112411251126112711281129113011311132113311341135113611371138113911401141114211431144114511461147114811491150115111521153115511561157115911601161116211631164117011711172120912111213121412151216121712181219122012211222122312241225122612271228122912301231123212331234123512361237123812391240124112421243124412451251125212531254 

Choi, K., Ueki, M., Imai, A., Kim, B., Kawabata, Z. 2004 Photoalternation of dissolved organic matter (DOM) released from Microcystis aeruginosa in different growth phases: DOM-fraction distribution and biodegradability. Arch. Hydrobiol., 159, 271-286.
Keywords: algal DOM; growth phase; UV effects; photoalteration; biodegradability; chemical composition
Strain(s): 843 

Otsuka, S., Suda, S., Shibata, S., Oyaizu, H., Matsumoto, S., Watanabe, M. M. 2001 A proposal for the unification of five species of the cyanobacterial genus Microcystis Kützing ex Lemmermann 1907 under the rules of the Bacteriological Code. Int. J. Syst. Evol. Microbiol., 51, 873-879.
Keywords: cyanobacteria; DNA-DNA hybridization; Microcystis; taxonomy; unification
Strain(s): 843 

Otsuka, S., Suda, S., Li, R., Matsumoto, S., Watanabe, M. M. 2000 Morphological variability of colonies of Microcystis morphospecies in culture. J. Gen. Appl. Microbiol., 46, 39-50.
Keywords: colony; cyanobacteria; Microcystis; morphology; phycoerythrin; pigment
Strain(s): 843901 

Suda, K., Tanji, K., Hori, K., Unno, H. 1999 Evidence for a novel Chlorella virus -encoded alginate lyase. FEMS Microbiol. Lett., 180, 45-53.
Keywords: Chlorella; virus; alginate lyase; lysis
Strain(s): 6868432170 

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