The Microbial Culture Collection at the National Institute for Environmental Studies

Strain Data


Strain number NIES-123  
Phylum Chlorophyta   Class Chlorophyceae 
Scientific name Rusalka fusiformis (Matvienko) Nakada  
Synonym Chlorogonium fusiforme Matvienko  
Former name Chlorogonium metamorphum Skuja (in IAM)  
Common name Green alga  
Locality (Date of collection) Lake Shinsen-numa Niseko Hokkaido Japan (1964-07-30)  
Habitat (Isolation source) Freshwater  
History < IAM (1983);  
Isolator (Date of isolation) Ichimura, Terunobu (1964-07-30)  
Identified by Ichimura, Terunobu; Nozaki, Hisayoshi; Nakada, Takashi (Reidentify)  
State of strain Unialgal; Clonal; Axenic[2012 Dec]  
Preservation state
(State of preservation)
Subculture  
Culture condition
( Preculture condition )
Medium:  AF-6  
Temperature: 20(25) C
Light intensity: 4-10(70-80) µmol photons/m2/sec     L/D cycle: 12L:12D
Duration: 2 M(14 D)  
Gene information atpB ( AB084329 ) , psaB ( AB084370 ) , rbcL ( AB010242 ) , 18S rRNA ( AB360750 )
Cell size (min - max) - 18 μm   Organization Unicellular; Flagellate 
Characteristics Homothallic; Authentic strain; Type specimen (NIES-50007, Epitype)  
Other strain no. Other collection strain no. : IAM C-349 (=C-569)
Other strain no. : MKF-14  
Remarks  

Reference
273)   Ichimura, T., Itoh, T. 1977 17. Preservation methods of microalgae (I) [17. Bisaisôrui no hozonhô (I)]. In Preservation methods of microorganisms [Biseibutsu Hozonhô], Ed. by Nei, T., University of Tokyo Press, Tokyo, p. 355-373 (in Japanese without English title). , Strain(s): 19212223242728303132333436373839404144454647484950122123127131144152173175179180181182183185198200203206217226227241266267268271295309310334338379436448450455527 

635)   Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55. , Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection , Strain(s): 19212223242526272829303132333435363738394041444546505152535455565758596061626364656667686970737475767778798081878990919293949596979899100101102103104105106107108109110111112119120122123124125127128129130131132133134135137138139144147148150151152153154155156157158159160162163164165166167168169170171172173174175176177178179180181182183185186187188189191192193194195196197198199200201202203204205206207208209210211212213214215216217224226227228229230231232241242243244245246248257259261262263266267268271287288289290294295297298299300301302303305306308309310311312313329334336337338339340341342349351359360361362375378379380382384385390394396397398415416418419421422423424425426427428429430431432433434436437438439440446447448449450451452453454455456457458459460464468469474478479480481503504505506507509510512514515522523524527528529530531532536537538539540541542543544545546564565566567568569570571572573574575576577578579580581582583584585592593594595596597598604610628630631632633634635636637638639640641642643644645646647648649650651652653654655656657658659660661662663664665666667672685686687688689690691692693694717718719720721722723724725726727728729 

680)   Nakada, T., Nozaki, H., Pröschold, T. 2008 Molecular phylogeny, ultrastructure, and taxonomic revision of Chlorogonium (Chlorophyta): emendation of Chlorogonium and description of Gungnir gen.nov. and Rusalka gen.nov. J. Phycol., 44, 751-760. , Keywords: Chlorogonium; Gungnir gen.nov.; molecular phylogeny; morphology; Rusalka gen.nov.; taxonomy; ultrastructure; Volvocales , Strain(s): 123439692751755761135713591360 

785)   Nozaki, H., Aizawa, K., Watanabe, M. M. 1996 Re-examination of two NIES strains labeled Chlorogonium metamorphum (Volvocales, Chlorophyta) from Japan. Nova Hedwigia Beiheft, 112, 483-490. , Keywords: Chlorogonium; Chlorophyta; morphology; neotype strain; species identification; Volvocales , Strain(s): 123446 

811)   Nozaki, H., Ohta, N., Morita, E., Watanabe, M. M. 1998 Toward a natural system of species in Chlorogonium (Volvocales, Chlorophyta): a combined analysis of morphological and rbcL gene sequence data. J. Phycol., 34, 1024-1037. , Keywords: Chlorogonium; Chlorogonium kasakii sp. nov.; Chlorophyta; molecular phylogeny; monophyletic species; morphology; natural systematics; rbcL gene; species taxonomy; Volvocales , Strain(s): 123439446692742743744745746747748749750751752753754755756757758759760761 

814)   Nozaki, H., Onishi, K., Morita, E. 2002 Differences in pyrenoid morphology are correlated with differences in the rbcL genes of members of the Chloromonas lineage (Volvocales, Chlorophyceae). J. Mol. Evol., 55, 414-430. , Keywords: ATP synthase β-subunit (atpB) gene; Chloromonas; gene evolution; large subunit of Rubisco (rbcL) gene; morphological evolution; P700 chlorophyll a-apoprotein A2 (psaB) gene; pyrenoid; Volvocales , Strain(s): 96123144213418421425428432439446447456474522564566567569571574582653732851858861875882883968