Strain number NIES-73  
Phylum Cyanophyta  
Class Cyanophyceae  
Scientific name Sphaerospermopsis kisseleviana (Elenkin) Zapomělová, Jezberová, Hrouzek, Hisem, Řeháková & Komárková  
Synonym Anabaena kisseleviana Elenkin; Sphaerospermum kisselevianum (Elenkin) Zapomelová et al. 2009  
Former name Formerly identified as Dolichospermum flos-aquae (=Anabaena flos-aquae), re-identified at NIES by DNA analysis[2016 Jul]  
Common name Blue-green alga ; Cyanobacteria  
Locality (Date of collection) Lake Kasumigaura Ibaraki Japan (1978-08-**)  
Latitude / Longitude 36.0428477398 / 140.373233439 
Habitat (Isolation source) Freshwater (Lake water)  
History < TAC;  
Isolator (Date of isolation) Watanabe, Masayuki (1978-08-**)  
Identified by Re-identified by NIES  
State of strain Subculture; Unialgal; Clonal; Axenic[2014 Jan.]  
Culture condition
(Preculture condition)
Medium:  MA  
Temperature:  22 C
Light intensity:  20-25 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  1 M  
Gene information Whole-genome ( AP018314 ) , 16S rRNA ( AB042858 AY701573 ) , pcB, pcA ( AY702243 )  
Cell size (min - max) 5 - 7 μm  
Organization Filamentous 
Characteristics Cyanobacterial water bloom (aoko) ; Planktonic ; Genome decoded strain  
Other strain no. Other strain no. : TAC 32 (K-TAN-32)  
Remarks Unstable; Axenic 
Movie  
Reference
Imanishi, S. Y., Nakayama, T., Asukabe, H., Harada, K. 2017 Application of MALDI Biotyper to cyanobacterial profiling. Rapid Commun. Mass Spectrom., 31, 325-332.
Strain(s): 738910210310711229861180880983584398910251043105810711099164216521672 
PubMed: 27862451
DOI: 10.1002/rcm.7793

Nagai, T., Taya, K., Annoh, H., Ishihara, S. 2013 Application of a fluorometric microplate algal toxicity assay for riverine periphytic algal species. Ecotox. Environ. Safe., 94, 37-44.
Keywords: Attached algae; In-vivo fluorescence; Pesticides; Species sensitivity distribution
Strain(s): 357173414487512797 
DOI: 10.1016/j.ecoenv.2013.04.020

Harada, K-I., Ozaki, K., Tsuzuki, S., Kato, H., Hasegawa, M., Kuroda, E. K., Arii, S., Tsuji, K. 2009 Blue color formation of cyanobacteria with b-cyclocitral. J. Chem. Ecol., 35, 1295-1301.
Keywords: b-cyclocitral; cyanobacteria; lysis; blue color formation; acid stress
Strain(s): 73102103112298611808 

Ozaki, K., Ohta, A., Iwata, C., Horikawa, A., Tsuji, K., Ito, E., Ikai, Y., Harada, K-I. 2008 Lysis of cyanobacteria with volatile organic compounds. Chemosphere, 71, 1531-1538.
Keywords: Cyanobacteria; Lysis; Microorganism; Volatile compound; b-Cyclocitral
Strain(s): 73102103112298 
PubMed: 18179811
DOI: 10.1016/j.chemosphere.2007.11.052

Hosoya, R., Hamana, K., Isobe, M., Yokota, A. 2005 Polyamine distribution profiles within Cyanobacteria. Microbiol. Cult. Coll., 21, 3-8.
Keywords: cyanobacteria; homospermidine; polyamine; spermidine
Strain(s): 41507378798081512515592593610846847 

Li, R., Watanabe, M. M. 2004 Fatty acid composition of planktonic species of Anabaena (Cyanobacteria) with coiled trichomes exhibited a significant. Curr. Microbiol., 49, 376-380.
Strain(s): 73767779263 
PubMed: 15486714
DOI: 10.1007/s00284-004-4380-x

Oikawa, E. Ishibashi, Y., 2004 Species specificity of musty odor producing Phormidium tenue in Lake Kamafusa. Wat. Sci. Tech., 49, 41-46.
Keywords: 16S-rDNA sequence; 2-methylisoborneol; musty odor; Phormidium tenue phylogenetic tree
Strain(s): 213033417376512611 
PubMed: 15237605

Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55.
Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection
Strain(s): 19212223242526272829303132333435363738394041444546505152535455565758596061626364656667686970737475767778798081878990919293949596979899100101102103104105106107108109110111112119120122123124125127128129130131132133134135137138139144147148150151152153154155156157158159160162163164165166167168169170171172173174175176177178179180181182183185186187188189191192193194195197198199200201202203204205206207208209210211212213214215216217224226227228229230231232241242243244245246248257259261262263266267268271287288289290294295297298299300301302303305306308309310311312313329334336337338339340341342349351359360361362375378379380382384385390394396397398415416418419421422423424425426427428429430431432433434436437438439440446447448449450451452453454455456457458459460464468469474478479480481503504505506507509510512514515522523524527528529530531532536537538539540541542543544545546564565566567568569570571572573574575576577578579580581582583584585592593594595596597598604610628630631632633634635636637638639640641642643644645646647648649650651652653654655656657658659660661662663664665666667672685686687688689690691692693694717718719720721722723724725726727728729 

Yuki, Y., Yoshida, T., Hiroishi, S. 2002 Molecular detection of the toxic cyanobacteria [Yûdoku aoko no bunshi-shikibetsu to yosatsu he-no ôyô]. In Series of Fisheries Science Vol. 143, Prevention and Extermination Strategies of Harmful Algal Blooms [Suisan-gaku shirîzu 134, Yûgai-yûdoku sôrui burûmu no yobô to kujo] , Eds. by Hiroishi, S., Imai, I. & Ishimaru, T., Koseisha-Koseikaku, Tokyo, p. 43-53 (in Japanese without English title).
Strain(s): 738187888990919899100101102104105106108109110111112204230298299604 

Lyra, C., Suomalainen, S., Gugger, M., Vezie, C., Sundman, P., Paulin, L., Sivonen, K. 2001 Molecular characterization of planktic cyanobacteria of Anabaena, Aphanizomenon, Microcystis and Planktothrix genera. Int. J. Syst. Evol. Microbiol., 51, 13-526.
Keywords: cyanobacteria; 16S rRNA gene; RFLP; sequencing; REP; ERIC
Strain(s): 41447379818998102104 
DOI: 10.1099/00207713-51-2-513

Oikawa, E., Ishibashi, Y., Abe, T., Umetsu, H. 2000 Phylogenetic classification of musty odor and/or toxic compounds producing cyanobacteria. Environ. Eng. Res., 37, 183-191 (in Japanese with English summary).
Keywords: cyanobacteria; musty odor; 16S ribosomal RNA; PCR; phylogenetic trees
Strain(s): 213033417376512 

Li, R., Watanabe, M., Watanabe, M. M. 2000 Taxonomic studies of planktic species of Anabaena based on morphological characteristics in cultured strains. Hydrobiologia, 438, 117-138.
Keywords: Anabaena; cyanobacteria; identification; morphology; planktic; taxonomy
Strain(s): 40737476777879802631652165316671672167316771695 

Park, H-D., Watanabe, M. F. 1996 Toxic Microcystis in eutrophic lakes. In Toxic Microcystis, Eds. by Watanabe, M.F., Harada, K., Carmichael W.W. & Fujii, H., CRC Press, Boca Raton, p. 57-77.
Strain(s): 737410631074107910851086109011543349 

Neilan, B. A., Jacobs, D., Goodman, A. E. 1995 Genetic diversity and phylogeny of toxic cyanobacteria determined by DNA polymorphisms within the phycocyanin locus. Appl. Environ. Microbiol., 61, 3875-3883.
Strain(s): 244041447376788081878998102107111112204207298 
PubMed: 8526499

Park, H-D., Hayashi, H. 1994 Microcystis and microcystins [Microcystis zoku to mikuroshisuchin]. In Waterbloom of Blue-green Algae and Their Toxins [Aoko, sono shutsugen to dokuso], Eds. by Watanabe, M. F., Harada,K. & Fujii, H., University of Tokyo Press, p. 75-99 (in Japanese without English title).
Strain(s): 7374105310541055105910611063106410661067106810701071107210741076107710791080108110831084108510861090109110931095109710981099114011543349 

Kasai, F., Takamura, N., Hatakeyama, S. 1993 Effects of simetryne on growth of various freshwater algal taxa. Environ. Pollut., 79, 77-83.
Strain(s): 35447377102137151230231255275280298 

Park, H-D., Watanabe, M. F., Harada, K., Nagai, H., Suzuki, M., Watanabe, M., Hayashi, H. 1993 Hepatotoxin (microcystin) and neurotoxin (anatoxin-a) contained in natural blooms and strains of cyanobacteria from Japanese freshwaters. Natural Toxins, 1, 353-360.
Keywords: Anabaena; anatoxin-a; neurotoxin; Microcystis; microcystin; hepatotoxin; natural bloom; strain; Japanese freshwaters; simultaneous analysis of anatoxin-a and microcystin
Strain(s): 73741063107410791085108610903349 

Soma, Y., Imaizumi, T., Yagi, K., Kasuga, S. 1993 Estimation of algal succession in lake water using HPLC analysis of pigments. Can. J. Fish. Aquat. Sci., 50, 1142-1146.
Strain(s): 44738188104111233234333351 

Saito, K., Matsumoto, M., Sekine, T., Murakoshi, I. 1989 Inhibitory substances from Myriophyllum brasiliense of growth of blue-green algae. J. Nat. Prod., 52, 1221-1226.
Strain(s): 4473 
DOI: 10.1021/np50066a004

Watanabe, M. M., Kasai, F., Hiwatari, T., Suda, S., Nei, T. 1984 Cryopreservation of microalgae in liquid nitrogen - Survival of algal cultures after freezing. Jpn. J. Freez. Dry., 30, 23-26 (in Japanese with English title).
Strain(s): 1819212426293139404144454650737475767780818788899091939598991001011201221311351371441512042072132572633794152219223522392579 

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