Strain number NIES-40  
Phylum Cyanophyta  
Class Cyanophyceae  
Scientific name Dolichospermum affine (Lemmermann) Wacklin, Hoffmann & Komárek  
Synonym Anabaena affinis Lemmermann  
Former name Anabaena affinis Lemmermann  
Common name Blue-green alga ; Cyanobacteria  
Locality (Date of collection) Lake Kasumigaura Ibaraki Japan (1974-08-**)  
Latitude / Longitude 36.0428477398 / 140.373233439 
Habitat (Isolation source) Freshwater (Lake water)  
History < IAM (1983);  
Isolator (Date of isolation) Watanabe, Makoto M.; Ichimura, Terunobu (1974-08-**)  
Identified by Watanabe, Makoto M.  
State of strain Subculture; Unialgal; Clonal; Non-axenic  
Culture condition
(Preculture condition)
Medium:  CT  
Temperature:  22 C
Light intensity:  20-25 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  1 M  
Gene information 16S rRNA ( AY701541 AF247591 ) , pcB-pcA ( AF427973 AY702211 )  
Cell size (min - max) 5 - 9 μm  
Organization Filamentous 
Characteristics Cyanobacterial water bloom (aoko)  
Other strain no. Other collection strain no. : IAM M-168
 
Remarks Unstable 
Movie  
Reference
Hodoki, Y., Ohbayashi, K., Kobayashi, Y., Okuda, N., Nakano, S. 2012 Detection and identification of potentially toxic cyanobacteria: Ubiquitous distribution of Microcystis aeruginosa and Cuspidothrix issatschenkoi in Japanese lakes. Harmful Algae, 16, 49-57.
Keywords: Anatoxin-a; Automated rRNA intergenic spacer analysis (ARISA); Cuspidothrix issatschenkoi; Cyanotoxin; Microcystin; Microcystis aeruginosa
Strain(s): 304077102111230512843905928932937991164516691698172919502116 
DOI: 10.1016/j.hal.2012.01.003

Baker, J. A., Entsch, B., McKay, D. B. 2003 The cyanobiont in an Azolla fern is neither Anabaena nor Nostoc. FEMS Mircobiol. Lett., 229, 43-47.
Keywords: Azolla fern; Cyanobacterial symbiont; Phylogenetic relationship
Strain(s): 4080 
PubMed: 14659541
DOI: 10.1016/S0378-1097(03)00784-5

Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55.
Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection
Strain(s): 19212223242526272829303132333435363738394041444546505152535455565758596061626364656667686970737475767778798081878990919293949596979899100101102103104105106107108109110111112119120122123124125127128129130131132133134135137138139144147148150151152153154155156157158159160162163164165166167168169170171172173174175176177178179180181182183185186187188189191192193194195197198199200201202203204205206207208209210211212213214215216217224226227228229230231232241242243244245246248257259261262263266267268271287288289290294295297298299300301302303305306308309310311312313329334336337338339340341342349351359360361362375378379380382384385390394396397398415416418419421422423424425426427428429430431432433434436437438439440446447448449450451452453454455456457458459460464468469474478479480481503504505506507509510512514515522523524527528529530531532536537538539540541542543544545546564565566567568569570571572573574575576577578579580581582583584585592593594595596597598604610628630631632633634635636637638639640641642643644645646647648649650651652653654655656657658659660661662663664665666667672685686687688689690691692693694717718719720721722723724725726727728729 

Li, R., Watanabe, M., Watanabe, M. M. 2000 Taxonomic studies of planktic species of Anabaena based on morphological characteristics in cultured strains. Hydrobiologia, 438, 117-138.
Keywords: Anabaena; cyanobacteria; identification; morphology; planktic; taxonomy
Strain(s): 40737476777879802631652165316671672167316771695 

Beltran, E. C., Neilan, B. 2000 Geographical segregation of the neurotoxin-producing cyanobacterium Anabaena circinalis. Appl. Environ. Microbiol., 66, 4468-4474.
Strain(s): 192123404180 
PubMed: 11010900
DOI: 10.1128/AEM.66.10.4468-4474.2000

Li, R., Yokota, A., Sugiyama, J., Watanabe, M., Hiroki, M., Watanabe, M. M. 1998 Chemotaxonomy of planktonic cyanobacteria based on non-polar and 3-hydroxy fatty acid composition. Phycol. Res., 46, 21-28.
Keywords: 3-hydroxy fatty acids; chemotaxonomy; fatty acid; non-polar fatty acids; planktonic cyanobacteria
Strain(s): 2740447678798081878998102104111112204205207298604810821825826 
DOI: 10.1111/j.1440-1835.1998.tb00093.x

Neilan, B. A., Jacobs, D., Goodman, A. E. 1995 Genetic diversity and phylogeny of toxic cyanobacteria determined by DNA polymorphisms within the phycocyanin locus. Appl. Environ. Microbiol., 61, 3875-3883.
Strain(s): 244041447376788081878998102107111112204207298 
PubMed: 8526499

Yamamoto, Y., Suzuki, K. 1990 Distribution and algal-lysing activity of fruiting myxobacteria in Lake Suwa. J. Phycol., 26, 457-462.
Keywords: algal-lysing activity; cyanobacteria; fruiting myxobacteria
Strain(s): 19404499102112 
DOI: 10.1111/j.0022-3646.1990.00457.x

Watanabe, M. M., Kasai, F., Hiwatari, T., Suda, S., Nei, T. 1984 Cryopreservation of microalgae in liquid nitrogen - Survival of algal cultures after freezing. Jpn. J. Freez. Dry., 30, 23-26 (in Japanese with English title).
Strain(s): 1819212426293139404144454650737475767780818788899091939598991001011201221311351371441512042072132572633794152219223522392579 

Ichimura, T., Itoh, T. 1977 17. Preservation methods of microalgae (I) [17. Bisaisôrui no hozonhô (I)]. In Preservation methods of microorganisms [Biseibutsu Hozonhô], Ed. by Nei, T., University of Tokyo Press, Tokyo, p. 355-373 (in Japanese without English title).
Strain(s): 19212223242728303132333436373839404144454647484950122123127131144152173175179180181182183185198200203206217226227241266267268271295309310334338379436448450455527 

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