Strain number NIES-88  
Phylum Cyanophyta  
Class Cyanophyceae  
Scientific name Microcystis aeruginosa (Kützing) Lemmermann  
Synonym Microcystis ichthyoblabe Kützing; Microcystis novacekii (Komárek) Compère; Microcystis viridis (A.Brown) Lemmermann; Microcystis wesenbergii Komárek  
Former name Microcystis aeruginosa (Kützing) Lemmermann f. aeruginosa  
Common name Blue-green alga ; Cyanobacteria  
Locality (Date of collection) Lake Kawaguchi Yamanashi Japan (1981-06-**)  
Latitude / Longitude 35.5130910551 / 138.748515637 
Habitat (Isolation source) Freshwater (Lake water)  
History < Watanabe, Makoto M.  
Isolator (Date of isolation) Watanabe, Michiko H. (1981-06-**)  
Identified by Watanabe, Michiko H.  
State of strain Cryopreservation; Unialgal; Clonal; Non-axenic  
Culture condition
(Preculture condition)
Medium:  MA  
Temperature:  25 C
Light intensity:  20-30 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  20 D  
Gene information Whole-genome ( JXYX00000000 ) , 16S rRNA ( AB023255 ) , ftsZ ( AB324852 ) , glnA ( AB324931 ) , gltX ( AB325010 ) , gyrB ( AB325089 ) , mcyA ( AB110103 ) , mcyD ( AB110114 ) , mcyG ( AB110125 ) , mcyJ ( AB110136 ) , pgi ( AB325168 ) , recA ( AB325247 ) , tpi ( AB325326 )  
Cell size (min - max) - 5 μm  
Organization  
Characteristics Cyanobacterial water bloom (aoko) ; Toxicity ; ST3 (Tanabe, Y. et al. 2007) ; Genome decoded strain (Parajuli, A. et al. 2016)  
Other strain no. Other strain no. : KW-MA1-3  
Remarks Toxicity; Cryopreserved 
Movie  
Reference
Sun, L.W., Jiang, W.J., Sato, H., Kawachi, M., Lu, X.W. 2016 Rapid classification and identification of Microcystis aeruginosa strains using MALDI-TOF MS and polygenetic analysis. PLoS One, 11, e0156275 (article ID).
Strain(s): 44878889901001011041051071091111124786048439011043105310581061106810691070107210761077108510941099110111041105111311151126113411351144115111571170121112131215121612171218122212241230123212331239 
DOI: 10.1371/journal.pone.0156275

Parajuli, A., Kwak, D. H., Dalponte, L., Leikoski, N., Galica, T., Umeobika, U., Trembleau, L., Bent, A., Sivonen, K., Wahlsten, M. 2016 A unique tryptophan c-prenyltransferase from the kawaguchipeptin biosynthetic pathway. Angew. Chem.-Int. Edit., 128, 3660–3663.
Strain(s): 88 
DOI: 10.1002/anie.201509920

Kimura, J. 2011 Screening for allelopathic activity on algal growth. Bull. Hiroshima Pref. Tech. Res. Inst., Publ. Health Environ. Center, 19, 37-46 (in Japanese).
Keywords: Microcystis; Chlorella; Nitzschia; growth inhibition; allelopathy
Strain(s): 88487642 

Tanabe, Y., Watanabe, M. M. 2011 Local expansion of a panmictic lineage of water bloom-forming cyanobacterium Microcystis aeruginosa. PLoS One, 6, e17085 (article ID).
Strain(s): 448788899091989910010110210310410510610710810911011111229829947860484390110501051105210531054105510561057105810591060106110621063106410651066106710681069107010721075107610771085109010911092109310941095109610971098110011011102110311041105110611071108111311141115112211231124112511261127112811291130113111321133113411351136113711381139114011411142114311441145114611471148114911501151115211531155115611571159116011611162116311641170117111721173117411751176117711791180118111831184118511861209121112131214121512161217121812191220122112221223122412251226122712281229123012311232123312341235123612371238123912401241124212431244124512511252125312542088208920902091246524662467246824692470247124722473247424752476247724782479248024812482248324842485248624872488248924902491249224932494249524962546254725482549255025512552255325542555255625572558255925602561259125922593259425952596259725982599260026012602260326042605260626072608260926102611261226132617261826192620262126222623262426252626262726282629 

Kimura, J. 2011 Screening for allelopathic activity on algal growth. Bull. Hiroshima Pref. Tech. Res. Inst., Publ. Health Environ. Center, 19, 37-46 (in Japanese with English title).
Keywords: Microcystis; Chlorella; Nitzschia; growth inhibition; allelopathy
Strain(s): 88487642 

Tanabe, Y., Sano, T., Kasai, F., Watanabe, M. M. 2009b Recombination, cryptic clades and neutral molecular divergence of the microcystin synthetase (mcy) genes of toxic cyanobacterium Microcystis aeruginosa. BMC Evol. Biol., 9, 115 (article ID).
Strain(s): 44878889909198991001011021031041051061071081091101111122982994786048439011050105110521053105410551056105710581059106010611062106310641065106610671068106910701072107510761077108510901091109210931094109510961097109811001101110211031104110511061107110811131114111511221123112411251126112711281129113011311132113311341135113611371138113911401141114211431144114511461147114811491150115111521153115511561157115911601161116211631164117011711172120912111213121412151216121712181219122012211222122312241225122612271228122912301231123212331234123512361237123812391240124112421243124412451251125212531254246524662467246924702471247224732474247524762477247824792561 
DOI: 10.1186/1471-2148-9-115

Jang, M.-H., Jung, J.-M., Takamura, N. 2008 Microcystin production by Microcystis aeruginosa exposed to different stages of herbivorous zooplankton. Toxicon, 51, 882-889.
Keywords: Microcystis aeruginosa; induced defense; different stages of zooplankton; infochemicals; intracellular microcystin; extracellular microcystin
Strain(s): 8898103107 

Kubo, T., Kato, N., Hosoya, K., Kaya, K. 2008 Effective determination method for a cyanobacterial neurotoxin, β-N-methylamino-L-alanine. Toxicon, 51, 1264-1268.
Keywords: Cyanobacteria; Neurotoxin; BMAA; LC–ESI-MS; HILIC; SIM
Strain(s): 88 

Jang, M.-H., Jung, J.-M., Takamura, N. 2007 Changes in microcystin production in cyanobacteria exposed to zooplankton at different population densities and infochemical concentrations. Limnol. Oceanogr., 52, 1454-1466.
Strain(s): 8898107 

Tanabe, Y., Kasai, F., Watanabe, M. M. 2007 Multilocus sequence typing (MLST) reveals high genetic diversity and clonal population structure of the toxic cyanobacterium Microcystis aeruginosa. Microbiology, 153, 3695-3703.
Strain(s): 44878889909198991001011021031041051061071081091101111122982994786048439019331050105110521053105410551056105710581059106010611062106310641065106610671068106910701072107510761077108510901091109210931094109510961097109811001101110211031104110511061107110811131114111511221123112411251126112711281129113011311132113311341135113611371138113911401141114211431144114511461147114811491150115111521153115511561157115911601161116211631164117011711172120912111213121412151216121712181219122012211222122312241225122612271228122912301231123212331234123512361237123812391240124112421243124412451251125212531254 

Jang, M.-H., Ha, K., Jung, J.-M., Lee, Y.-J., Takamura, N. 2006 Increased microcystin production of Microcystis aeruginosa by indirect exposure of nontoxic cyanobacteria: potential role in the development. Bull. Environ. Contam. Toxicol., 76, 957-962.
Strain(s): 7488204 

Yamaki, H., Sitachitta, N., Sano, T., Kaya, K. 2005 Two new chymotrypsin inhibitors isolated from the cyanobacterium Microcystis aeruginosa NIES-88. J. Nat. Prod., 68, 14-18.
Strain(s): 88 

Tsuda, K., Takamura, N., Matsuyama, M., Fujii, Y. 2005 Assessment method for leaf litters allelopathic effect on cyanobacteria. J. Aquat. Plant Manage, 43, 43-46.
Keywords: allelopathy; bioassay; leaf disk; Microcystis aeruginosa
Strain(s): 88 

Jang, M. H., Ha, K., Lucas, M. C., Joo, G. J., Takamura, N. 2004 Changes in microcystin production by Microcystis aeruginosa exposed to phytoplanktivorous and omnivorous fish. Aquat. Toxicol., 68, 51-59.
Keywords: Microcystis aeruginosa; microcystin; fish-induced defence; chemical cues; Hypophthalmichthys molitrix; Carassius gibelio langsdorfi
Strain(s): 448899 
PubMed: 15110469
DOI: 10.1016/j.aquatox.2004.02.002

Tanabe, Y., Kaya, K., Watanabe, M. M. 2004 Evidence for recombination in the microcystin synthetase (mcy) genes of toxic cyanobacteria Microcystis spp. J. Mol. Evol., 58, 633-641.
Keywords: cyanobacteria; microcystin synthetase; Microcystis; NRPS; recombination; split decomposition
Strain(s): 888990102103107 

Jang, M-H., Ha, K., Joo, G-J., Takamura, N. 2003 Toxin production of cyanobacteria is increased by exposure to zooplankton. Freshw. Biol., 48, 1540-1550.
Keywords: info-chemicals; microcystin production; Microcystis aeruginosa; zooplankton; zooplankton culture media filtrate
Strain(s): 44878899 

Yoshida, T., Yuki, Y., Lei, S., Chinen, H., Yoshida, M., Kondo, R., Hiroishi, S. 2003 Quantitative detection of toxic strains of the cyanobacterial genus Microcystis by competitive PCR. Microbes. Environ., 18, 16-23.
Keywords: competitive PCR; microcystin; Microcystis; peptide synthetase; toxic cyanobacteria
Strain(s): 87888990102104105106108109110111112298299604 

Yuki, Y., Yoshida, T., Hiroishi, S. 2002 Molecular detection of the toxic cyanobacteria [Yûdoku aoko no bunshi-shikibetsu to yosatsu he-no ôyô]. In Series of Fisheries Science Vol. 143, Prevention and Extermination Strategies of Harmful Algal Blooms [Suisan-gaku shirîzu 134, Yûgai-yûdoku sôrui burûmu no yobô to kujo] , Eds. by Hiroishi, S., Imai, I. & Ishimaru, T., Koseisha-Koseikaku, Tokyo, p. 43-53 (in Japanese without English title).
Strain(s): 738187888990919899100101102104105106108109110111112204230298299604 

Kondo, R., Kagiya, G., Hiroishi, S., Watanabe, M. 2000 Genetic typing of a bloom-forming cyanobacterial genus Microcystis in Japan using 16S rRNA gene sequence analysis. Plankton Biol. Ecol., 47, 1-6.
Keywords: genetic typing; 16S rDNA; Microcystis; cyanobacteria; phylogenetic analysis
Strain(s): 878889909198991001011021041051061081091101111122982996041055106110681076109010911105112211421164 

Kondo, R., Kagiya, G., Komura, M., Yuki, Y., Hiroishi, S., Watanabe, M. 2000 Molecular taxonomy of a bloom-forming cyanobacterial genus Microcystis. Bull. Plankton Soc. Japan, 47, 25-33 (in Japanese with English summary).
Keywords: cyanobacteria; Microcystis; bacterial criteria; molecular analysis
Strain(s): 6878889909198991001011021041051061081091101111122982996041055106110681076109010911105112211421164 

Kondo, R., Kagiya, G., Hiroishi, S., Watanabe, M., Hata, Y. 1999 Rapid DNA extraction from a bloom-forming cyanobacterium. Microbes. Environ., 14, 157-161.
Keywords: DNA extraction; PCR; Microcystis; cyanobacteria
Strain(s): 878889909198991001011021041051061081091101111122982996041055106110681076109010911105112211421164 

Ishida, K., Matsuda, H., Murakami, M. 1998 Micropeptins 88-A to 88-F, chymotrypsin inhibitors from the cyanobacterium Microcystis aeruginosa (NIES-88). Tetrahedron, 54, 5545-5556.
Strain(s): 88 

Kaya, K., Sano, T. 1998 A photodetoxifi-cation mechanism of the cyanobacterial hepatotoxin microcystin-LR by ultraviolet irradiation. Chem. Res. Toxicol., 11, 159-163.
Strain(s): 88 

Kondo, R., Kagiya, G., Yuki, Y., Hiroishi, S., Watanabe, M. 1998 Taxonomy of a bloom-forming cyanobacterial genus Microcystis. Nippon Suisan Gakkaishi, 64, 291-292 (in Japanese with English title).
Strain(s): 87888990919899100101102104105106107108109110111112298299604 

Ishida, K., Matsuda, H., Murakami, M., Yamaguchi, K. 1997 Kawaguchipeptin B, an antibacterial cyclic undecapeptide from the cyanobacterium Microcystis aeruginosa. J. Nat. Prod., 60, 724-726.
Strain(s): 88 

Ishida, K., Matsuda, H., Murakami, M., Yamaguchi, K. 1996 Kawaguchipeptin A, a novel cyclic undecapeptide from cyanobacterium Microcystis aeruginosa (NIES-88). Tetrahedron, 52, 9025-9030.
Strain(s): 88 

Soma, Y., Imaizumi, T., Yagi, K., Kasuga, S. 1993 Estimation of algal succession in lake water using HPLC analysis of pigments. Can. J. Fish. Aquat. Sci., 50, 1142-1146.
Strain(s): 44738188104111233234333351 

Tsuchiya, Y., Watanabe, M. F., Watanabe, M. 1992 Volatile organic sulfur compounds associated with blue-green algae from inland waters of Japan. Wat. Sci. Tech., 25, 123-130.
Keywords: blue-green algae; Microcystis aeruginosa; M. wesenbergii; isopropyl mercaptan; isopropyl disulfide; isopropyl sulfonyl chloride; chlorination; mutagenicity; Ames test
Strain(s): 881061108010811083109811041137 

Watanabe, M. M., Kasai, F., Hiwatari, T., Suda, S., Nei, T. 1984 Cryopreservation of microalgae in liquid nitrogen - Survival of algal cultures after freezing. Jpn. J. Freez. Dry., 30, 23-26 (in Japanese with English title).
Strain(s): 1819212426293139404144454650737475767780818788899091939598991001011201221311351371441512042072132572633794152219223522392579 

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