Strain number NIES-44  
Phylum Cyanophyta  
Class Cyanophyceae  
Scientific name Microcystis aeruginosa (Kützing) Lemmermann  
Synonym Microcystis ichthyoblabe Kützing; Microcystis novacekii (Komárek) Compère; Microcystis viridis (A.Brown) Lemmermann; Microcystis wesenbergii Komárek  
Former name Microcystis aeruginosa (Kützing) Lemmermann f. aeruginosa  
Common name Blue-green alga ; Cyanobacteria  
Locality (Date of collection) Lake Kasumigaura Ibaraki Japan (1974-08-**)  
Latitude / Longitude 36.0428477398 / 140.373233439 
Habitat (Isolation source) Freshwater  
History < IAM (1983);  
Isolator (Date of isolation) Watanabe, Makoto M. (1974-08-**)  
Identified by Watanabe, Makoto M.  
State of strain Cryopreservation; Unialgal; Clonal; Axenic  
Culture condition
(Preculture condition)
Medium:  CB  
Temperature:  25 C
Light intensity:  20-30 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  20 D  
Gene information Whole-genome ( BBPA00000000 ) , 16S-23S-ITS ( AB015361 AB015387 AB254437 ) , ftsZ ( AB324850 ) , glnA ( AB324929 ) , gltX ( AB325008 ) , gyrB ( AB325087 ) , pgi ( AB325166 ) , recA ( AB325245 ) , tpi ( AB325324 )  
Cell size (min - max) 6 - 8 μm  
Organization  
Characteristics Cyanobacterial water bloom (aoko) ; ST1 (Tanabe, Y. et al. 2007) ; Genome decoded strain (Okano, K. et al. 2015)  
Other strain no. Other collection strain no. : IAM M-176 (=M-228)
 
Remarks Cryopreserved 
Movie  
Reference
Xuan, H., Dai, X., Li, J., Zhang, X., Yang, C., Luo, F. 2017 A Bacillus sp. strain with antagonistic activity against Fusarium graminearum kills Microcystis aeruginosa selectively. Sci. Total Environ., 583, 214-221.
Keywords: Harmful algal blooms; Algicidal activity; Antagonism; Bifunctional biocontrol agent
Strain(s): 4490843 
PubMed: 28104332
DOI: 10.1016/j.scitotenv.2017.01.055

Ngoc, N.M., Inoue, Y., Yokota, K. 2017 Ultrasonic extraction method for quantifying bioavailable phosphorus in particulate form. J. Environ. Chem. Eng., 5, 2498-2507.
Keywords: Particulate phosphorus; Ultrasonic extraction; NaOH extraction; Algal growth potential; Bioavailable phosphorus; Microcystis aeruginosa
Strain(s): 44 
DOI: 10.1016/j.jece.2017.04.047

Ngoc, N.M., Yokota, K., James, M., Inoue, Y. 2017 The estimation of bioavailable phosphorus in particulate forms by ultrasonic treatment. J. Environ. Chem. Eng.,
Keywords: algal growth potential test; bioavailable phosphorus; Microcystis aeruginosa; particulate phosphorus; ultrasonic extraction
Strain(s): 44 
DOI: 10.1111/wej.12270

Klemenčič, M., Funk, C. 2017 Structural and functional diversity of caspase homologues in non-metazoan organisms. Protoplasma,
Strain(s): 448432549 
PubMed: 28744694
DOI: 10.1007/s00709-017-1145-5

Sun, L.W., Jiang, W.J., Sato, H., Kawachi, M., Lu, X.W. 2016 Rapid classification and identification of Microcystis aeruginosa strains using MALDI-TOF MS and polygenetic analysis. PLoS One, 11, e0156275 (article ID).
Strain(s): 44878889901001011041051071091111124786048439011043105310581061106810691070107210761077108510941099110111041105111311151126113411351144115111571170121112131215121612171218122212241230123212331239 
DOI: 10.1371/journal.pone.0156275

Okano, K., Miyata, N., Ozaki, Y. 2015 Whole genome sequence of the non-microcystin-producing Microcystis aeruginosa strain NIES-44. GenomeA, 3, e00135-15 (article ID).
Strain(s): 44 
DOI: 10.1128/genomeA.00135-15

Ohkouchi, Y., Tajima, S., Nomura, M., Itoh, S. 2015 Inflammatory responses and potencies of various lipopolysaccharides from bacteria and cyanobacteria in aquatic environments and water supply systems. Toxicon, 97, 23-31.
Keywords: Lipopolysaccharide (LPS); Purification; Endotoxin; Indigenous bacteria/cyanobacteria; Human monocytic cells; Cytokine secretion
Strain(s): 44946 
DOI: 10.1016/j.toxicon.2015.02.003

Kataoka, T., Homma, T., Nakano, S., Hodoki, Y., Ohbayashi, K., Kondo, R. 2013 PCR primers for selective detection of intra-species variations in the bloom-forming cyanobacterium, Microcystis. Harmful Algae, 23, 46-54.
Keywords: Microcystis aeruginosa; Quantitative PCR; 16S-23S rDNA ITS fegion; Selective detection; Intra-species variation
Strain(s): 4487909810110210310711111229860410621070 

Ninomiya, K., Ogino, C., Kawabata, S., Kitamura, K., Maki, T., Hasegawa, H., Shimizu, N. 2013 Ultrasonic inactivation of Microcystis aeruginosa in the presence of TiO2 particles. J. Biosci. Bioeng., 116, 214-218.
Keywords: Ultrasaound; Titanium dioxide; Disinfection; Microcystis aeruginosa; Hydroxyl radicals
Strain(s): 44 
DOI: 10.1016/j.jbiosc.2013.02.006

Kang, Y.-H., Park, C.-S., Han, M.-S. 2012 Pseudomonas aeruginosa UCBPP-PA14 a useful bacterium capable of lysing Microcystis aeruginosa cells and degrading microcystins. J. Appl. Phycol., 24, 1517-1525.
Keywords: Microcystis aeruginosa; Microcystins; Algicidal bacteria; Pseudomonas aeruginosa
Strain(s): 4494298 

Okubo, Y., Inoue, T., Yokoto, K. 2012 Estimating bioavailability of soil particulate phosphorus toMicrocystis aeruginosa. J. Appl. Phycol., 24, 1503-1507.
Keywords: Particulate phosphorus; Bioavailable phosphorus; Microcystis aeruginosa; Cyanobacteria; Sequential phosphorus extraction
Strain(s): 44 

Kaya, K., Shiraishi, F., Uchida, H., Sano, T. 2011 A novel retinoic acid analogue, 7-hydroxy retinoic acid, isolated from cyanobacteria. Biochim. Biophys. Acta, 1810, 414-419.
Keywords: 7-hydroxy retinoic acid; RAR agonistic activity; cyanobacteria; UV stability
Strain(s): 444546512610931 
DOI: 10.1016/j.bbagen.2010.11.009

Tanabe, Y., Watanabe, M. M. 2011 Local expansion of a panmictic lineage of water bloom-forming cyanobacterium Microcystis aeruginosa. PLoS One, 6, e17085 (article ID).
Strain(s): 448788899091989910010110210310410510610710810911011111229829947860484390110501051105210531054105510561057105810591060106110621063106410651066106710681069107010721075107610771085109010911092109310941095109610971098110011011102110311041105110611071108111311141115112211231124112511261127112811291130113111321133113411351136113711381139114011411142114311441145114611471148114911501151115211531155115611571159116011611162116311641170117111721173117411751176117711791180118111831184118511861209121112131214121512161217121812191220122112221223122412251226122712281229123012311232123312341235123612371238123912401241124212431244124512511252125312542088208920902091246524662467246824692470247124722473247424752476247724782479248024812482248324842485248624872488248924902491249224932494249524962546254725482549255025512552255325542555255625572558255925602561259125922593259425952596259725982599260026012602260326042605260626072608260926102611261226132617261826192620262126222623262426252626262726282629 

Oyama, Y., Matsushita, B., Fukushima, T., Chen, J., Nagai, T., Imai, A. 2010 Testing the spectral decomposition algorithm (SDA) for different phytoplankton species by a simulation based on tank experiments. Int. J. Remote Sens., 31, 1605-1623.
Strain(s): 4475204803 

Tanabe, Y., Sano, T., Kasai, F., Watanabe, M. M. 2009b Recombination, cryptic clades and neutral molecular divergence of the microcystin synthetase (mcy) genes of toxic cyanobacterium Microcystis aeruginosa. BMC Evol. Biol., 9, 115 (article ID).
Strain(s): 44878889909198991001011021031041051061071081091101111122982994786048439011050105110521053105410551056105710581059106010611062106310641065106610671068106910701072107510761077108510901091109210931094109510961097109811001101110211031104110511061107110811131114111511221123112411251126112711281129113011311132113311341135113611371138113911401141114211431144114511461147114811491150115111521153115511561157115911601161116211631164117011711172120912111213121412151216121712181219122012211222122312241225122612271228122912301231123212331234123512361237123812391240124112421243124412451251125212531254246524662467246924702471247224732474247524762477247824792561 
DOI: 10.1186/1471-2148-9-115

Jung, S. W., Kim, B.-H., Katano, T., Kong, D.-S., Han, M.-S. 2008 Pseudomonas flurescens HYK0210-SK09 offers species-specific biological control of winter algal blooms caused by freshwater diatom Stephanodiscus hantzschii. J. Appl. Microbiol., 105, 186-195.
Keywords: algicidal bacterium; algicidal mechanism; biological control; indoor mesocosm; Pseudomonas fluorescens; Stephanodiscus hantzschii
Strain(s): 1944298 

Shinohara, A., Imai, A., Komatsu, K., Matsushige, K., Nara, F. 2008 Application of HPLC-PAD to highly sensitive analysis of dissolved carbohydrates and theit composition in lake water and extracellular organic matter derived from algae. J. Jpn. Soc. Wat. Environ., 31, 447-454 (in Japanese with English summary).
Keywords: carbohydrates; DOM; HPLC-PAD; lake water; algal culture
Strain(s): 4474204 

Nagai, T., Imai, A., Matsushige, K., Fukushima, T. 2007 Growth characteristics and growth modeling of Microcystis aeruginosa and Planktothrix agardhii under iron limitation. Limnology, 8, 261-270.
Keywords: iron; growth rate; uptake rate; model simulation; cyanobacteria
Strain(s): 44204 

Tanabe, Y., Kasai, F., Watanabe, M. M. 2007 Multilocus sequence typing (MLST) reveals high genetic diversity and clonal population structure of the toxic cyanobacterium Microcystis aeruginosa. Microbiology, 153, 3695-3703.
Strain(s): 44878889909198991001011021031041051061071081091101111122982994786048439019331050105110521053105410551056105710581059106010611062106310641065106610671068106910701072107510761077108510901091109210931094109510961097109811001101110211031104110511061107110811131114111511221123112411251126112711281129113011311132113311341135113611371138113911401141114211431144114511461147114811491150115111521153115511561157115911601161116211631164117011711172120912111213121412151216121712181219122012211222122312241225122612271228122912301231123212331234123512361237123812391240124112421243124412451251125212531254 

Susilaningsih, D. 2007 Accelerating of pink pigment excretion from cyanobacterium Oscillatoria by Co-Cultivation with Anabaena. HHAYATI J. Biosci., 14, 18-22.
Keywords: Oscillatoria; Anabaena variabilis; biosurfactant; cyanobacterium; pink pigment
Strain(s): 192344422 

Ando, T., Nagase, H., Eguchi, K., Hirooka, T., Nakamura, T., Miyamoto, K., Hirata, K. 2007 A novel method using cyanobacteria for ecotoxicity test of veterinary antimicrobial agents. Environ. Toxicol. Chem., 26, 601-606.
Keywords: Antimicrobial agent; Cyanobacterium; Ecotoxicity test; Algal toxicity
Strain(s): 192344107 

Nagai, T., Imai, A., Matsushige, K., Fukushima, T. 2006 Effect of iron complexation with dissolved organic matter on the growth of cyanobacteria in a eutrophic lake. Aquat. Microb. Ecol., 44, 231-239.
Keywords: dissolved organic matter; DOM; cyanobacteria; iron; organic complexation
Strain(s): 44204 

Yoshida, T., Takashima, Y., Tomaru Y., Shirai, Y., Takao, Y., Hiroishi, S., Nagasaki, K. 2006 Isolation and characterization of a cyanophage infecting the toxic cyanobacterium Microcystis aeruginosa. Appl. Environ. Microbiol., 72, 1239-1247.
Strain(s): 44879098102104111112298604 

Tezuka, M., Satou, Y., Nakamura, M. 2005 The effect of phosphorus in the fine-grained soil on the eutrophication of closed water area. J. JSCE [Doboku-kôgaku Ronbunshû], 804 (VII-37), 113-123 (in Japanese with English summary).
Keywords: eutrophication; fine-grained soil; phosphorus
Strain(s): 44 

Nagai, T., Imai, A., Matsushige, K., Fukushima, T. 2005 Limiting nutrients on the growth of bloom-forming cyanobacteria with special focus on iron speciation. Verh. Internat. Verein. Limnol., 29, 949-952.
Keywords: limiting nutrient; algal growth potential test; speciation; iron
Strain(s): 44204 

Nagase, H., Inthorn, D., Oda, A., Nishimura, J., Kajiwara, Y., Park, M.-O., Hirata, K., Miyamoto, K. 2005 Improvement of selective removal of heavy metals in cyanobacteria by NaOH treatment. J. Biosci. Bioeng., 99, 372-377.
Keywords: cyanobacteria; cadmium; zinc; copper; lead; hard water
Strain(s): 2344 

Jang, M. H., Ha, K., Lucas, M. C., Joo, G. J., Takamura, N. 2004 Changes in microcystin production by Microcystis aeruginosa exposed to phytoplanktivorous and omnivorous fish. Aquat. Toxicol., 68, 51-59.
Keywords: Microcystis aeruginosa; microcystin; fish-induced defence; chemical cues; Hypophthalmichthys molitrix; Carassius gibelio langsdorfi
Strain(s): 448899 
PubMed: 15110469
DOI: 10.1016/j.aquatox.2004.02.002

Wada, Y., Yokotera, H., Yamazaki, K., Toyonaga, K. 2004 Study on the multiply capability of phytoplankton at the artificial repetition on blight and dark condition. Advance in River Engineering, Vol.10, 465-470 (in Japanese with English summary).
Keywords: destratification system; multiply capability; blight and dark condition; Microcystis; algal growth potential; record of light shield
Strain(s): 44 

Jang, M-H., Ha, K., Joo, G-J., Takamura, N. 2003 Toxin production of cyanobacteria is increased by exposure to zooplankton. Freshw. Biol., 48, 1540-1550.
Keywords: info-chemicals; microcystin production; Microcystis aeruginosa; zooplankton; zooplankton culture media filtrate
Strain(s): 44878899 

Hirooka, T., Akiyama, Y., Tsuji, N., Nakamura, T., Nagase, H., Hirata, K., Miyamoto, K. 2003 Removal of hazardous phenols by microalgae under photoautotrophic conditions. J. Biosci. Bioeng., 95, 200-203.
Keywords: green algae; cyanobacteria; nitrophenol; chlorophenol; bisphenol A; photoautotrophic conditions
Strain(s): 192344422437 

Asai, R., Ootani, K., Nomura, Y., Nakamura, C., Ikebukuro, K., Arikawa, Y., Miyake, J., Karube, I. 2003 PCR-based ribosomal DNA detection technique for microalga (Heterosigma carterae) causing red tide and its application to a biosensor using labeled probe. Mar. Biotechnol., 5, 417-423.
Keywords: small subunit rDNA; Heterosigma carterae; fluorescence polarization; asymmetric polymerase chain reaction; surface plasmon resonance
Strain(s): 144 
DOI: 10.1007/s10126-002-0081-2

Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55.
Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection
Strain(s): 19212223242526272829303132333435363738394041444546505152535455565758596061626364656667686970737475767778798081878990919293949596979899100101102103104105106107108109110111112119120122123124125127128129130131132133134135137138139144147148150151152153154155156157158159160162163164165166167168169170171172173174175176177178179180181182183185186187188189191192193194195197198199200201202203204205206207208209210211212213214215216217224226227228229230231232241242243244245246248257259261262263266267268271287288289290294295297298299300301302303305306308309310311312313329334336337338339340341342349351359360361362375378379380382384385390394396397398415416418419421422423424425426427428429430431432433434436437438439440446447448449450451452453454455456457458459460464468469474478479480481503504505506507509510512514515522523524527528529530531532536537538539540541542543544545546564565566567568569570571572573574575576577578579580581582583584585592593594595596597598604610628630631632633634635636637638639640641642643644645646647648649650651652653654655656657658659660661662663664665666667672685686687688689690691692693694717718719720721722723724725726727728729 

Choi, K., Imai, A., Kim, B., Matsushige, K. 2001 Properties of dissolved organic carbon (DOC) released by three species of blue-green algae. Korean J. Limnol., 34, 20-29.
Keywords: extracellular organic carbon; fractionation; blue-green algae; growth phases
Strain(s): 4474204 

Dwi, S., Hirata, K., Asada, Y., Miyamoto, K. 2001 Utilization of cyanobacterial biomass from water bloom for bioproduction of lactic acid. World J. Microbiol. Biotech., 17, 259-264.
Keywords: cyanobacterium; CO2 supplementation; lactic acid; Lactobacillus agilis; L. amylovorus; L. ruminis; starch; water bloom
Strain(s): 2344 

Lyra, C., Suomalainen, S., Gugger, M., Vezie, C., Sundman, P., Paulin, L., Sivonen, K. 2001 Molecular characterization of planktic cyanobacteria of Anabaena, Aphanizomenon, Microcystis and Planktothrix genera. Int. J. Syst. Evol. Microbiol., 51, 13-526.
Keywords: cyanobacteria; 16S rRNA gene; RFLP; sequencing; REP; ERIC
Strain(s): 41447379818998102104 
DOI: 10.1099/00207713-51-2-513

Otsuka, S., Suda, S., Li, R., Watanabe, M., Oyaizu, H., Matsumoto, S., Watanabe, M. M. 1999 Phylogenetic relationships between toxic and non-toxic strains of the genus Microcystis based on 16S to 23S internal transcribed spacer sequence. FEMS Mircobiol. Lett., 172, 15-21.
Keywords: cyanobacteria; Microcystis; internal transcribed spacer; phylogeny; microcystin
Strain(s): 44104111105410551058106710681072108510861090109110921105111511171143 

Li, R., Yokota, A., Sugiyama, J., Watanabe, M., Hiroki, M., Watanabe, M. M. 1998 Chemotaxonomy of planktonic cyanobacteria based on non-polar and 3-hydroxy fatty acid composition. Phycol. Res., 46, 21-28.
Keywords: 3-hydroxy fatty acids; chemotaxonomy; fatty acid; non-polar fatty acids; planktonic cyanobacteria
Strain(s): 2740447678798081878998102104111112204205207298604810821825826 

Hirata, K., Phunchindawan, M., Tukamoto, J., Goda, S., Miyamoto, K. 1996 Cryopreservation of microalgae using encapsulation-dehydration. CryoLetters, 17, 321-328.
Keywords: Dunaliella tertiolecta; microalgae; cryopreservation; encapsulation-dehydration
Strain(s): 8192224273244242375396422437 

Neilan, B. A., Jacobs, D., Goodman, A. E. 1995 Genetic diversity and phylogeny of toxic cyanobacteria determined by DNA polymorphisms within the phycocyanin locus. Appl. Environ. Microbiol., 61, 3875-3883.
Strain(s): 244041447376788081878998102107111112204207298 

Watanabe, M. M., Sawaguchi, T. 1995 Cryopreservation of a water-bloom forming cyanobacterium, Microcystis aeruginosa f. aeruginosa. Phycol. Res., 43, 111-116.
Keywords: cryopreservation; DMSO; Microcystis aeruginosa f. aeruginosa; rapid cooling; two step cooling
Strain(s): 4490 

Kasai, F., Takamura, N., Hatakeyama, S. 1993 Effects of simetryne on growth of various freshwater algal taxa. Environ. Pollut., 79, 77-83.
Strain(s): 35447377102137151230231255275280298 

Soma, Y., Imaizumi, T., Yagi, K., Kasuga, S. 1993 Estimation of algal succession in lake water using HPLC analysis of pigments. Can. J. Fish. Aquat. Sci., 50, 1142-1146.
Strain(s): 44738188104111233234333351 

Hanazato, T. 1991 Interrelations between Microcystis and Cladocera in the highly eutrophic Lake Kasumigaura, Japan. Int. Revue ges. Hydrobiol., 76, 21-36.
Keywords: Microcystis; Cladocera; toxicity; food chain; Lake Kasumigaura
Strain(s): 44298299 

Kang, C. B. H., Ho. K.-K. 1991 Characteri-zation of a soluble inorganic pyrophosphatase from Microcystis aeruginosa. Archi. Biochem. Biophys., 289, 281-288.
Strain(s): 44 

Yamamoto, Y., Suzuki, K. 1990 Distribution and algal-lysing activity of fruiting myxobacteria in Lake Suwa. J. Phycol., 26, 457-462.
Keywords: algal-lysing activity; cyanobacteria; fruiting myxobacteria
Strain(s): 19404499102112 

Tan, S., Ho, K.-K. 1989 Purification of an acidic plastocyanin from Microcystis aeruginosa. Biochim. Biophys. Acta, 973, 111-117.
Keywords: plastocyanin; cytochrome c-553; absorption chromatography; cyanobacteria; photosynthesis
Strain(s): 44 

Saito, K., Matsumoto, M., Sekine, T., Murakoshi, I. 1989 Inhibitory substances from Myriophyllum brasiliense of growth of blue-green algae. J. Nat. Prod., 52, 1221-1226.
Strain(s): 4473 

Kang, B.-H., Tan, S., Ho, K.-K. 1988 Isolation of water-soluble cytochromes from cyanobacteria by adsorption chromatography. Archi. Biochem. Biophys., 263, 387-393.
Strain(s): 44 

Asada, Y., Kawamura, S., Ho, K.-K. 1987 Hydrogenase from the unicellular cyano-bacterium, Microcystis aeruginosa. Phytochemistry, 26, 637-640.
Keywords: Microcystis aeruginosa; cyanobacterium; hydrogenase; purification; properties
Strain(s): 44 

Tachibana, H., Moriguchi, A., Inoue, T., Imaoka, T. 1986 Fundamental study on algal growth potential. II. Utilization of particulate nutrients by algae in river waters. Proc. Environ. Sanit. Eng. Res., 22, 151-161 (in Japanese).
Strain(s): 442170 

Watanabe, M. M., Kasai, F., Hiwatari, T., Suda, S., Nei, T. 1984 Cryopreservation of microalgae in liquid nitrogen - Survival of algal cultures after freezing. Jpn. J. Freez. Dry., 30, 23-26 (in Japanese with English title).
Strain(s): 1819212426293139404144454650737475767780818788899091939598991001011201221311351371441512042072132572633794152219223522392579 

Tachibana, H. 1984 Fundamental study on algal growth potential. Proc. Environ. Sanit. Eng. Res., 20, 53-60 (in Japanese).
Strain(s): 44 

Ichimura, T., Itoh, T. 1977 17. Preservation methods of microalgae (I) [17. Bisaisôrui no hozonhô (I)]. In Preservation methods of microorganisms [Biseibutsu Hozonhô], Ed. by Nei, T., University of Tokyo Press, Tokyo, p. 355-373 (in Japanese without English title).
Strain(s): 19212223242728303132333436373839404144454647484950122123127131144152173175179180181182183185198200203206217226227241266267268271295309310334338379436448450455527 

Ichimura, T., Watanabe, M. M. 1977 An axenic clone of Microcystis aeruginosa Kutz. emend. Elenkin from Lake Kasumigaura. Bull. Jpn. Soc. Phycol., 25, 177-181.
Strain(s): 44 

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