Strain number NIES-3773  
Phylum Chlorophyta  
Class Chlorophyceae  
Scientific name Pyrobotrys squarrosa (Korshikov) Korshikov  
Synonym Chlamydobotrys squarrosa Korshikov 1928; Uva squarrosa (Korshikov) Fott 1967; Pyrobotrys squarrosa (Korshikov) Huber-Pestalozzi in Fott 1967  
Former name  
Common name Green alga  
Locality (Date of collection) Akiragi, Hagi Yamaguchi Japan (2013-01-08)  
Latitude / Longitude 34.347111 / 131.419667 
Habitat (Isolation source) Freshwater (Sediment)  
History < Sugasawa, Mizuho  
Isolator (Date of isolation) Sugasawa, Mizuho (2014-04-08)  
Identified by Sugasawa, Mizuho  
State of strain Subculture; Unialgal; Clonal  
Culture condition
(Preculture condition)
Medium:  AF-6  
Temperature:  20 C
Light intensity:  24-35 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  21 D  
Gene information 18S rRNA ( LC093474 ) , rbcL ( LC093475 ) , psaB ( LC093476 )  
Cell size (min - max)  
Organization Colonial; Flagellate 
Characteristics Mixotrophic ; Osmotrophic ; Phototaxis ; Microaerophilic  
Other strain no. Other strain no. : YS11  
Remarks Fragile species to transportation stresses; Read and agree "How to order 4.1"; Culturing under microaerophilic condition 
Movie  
Reference
Sugasawa, M., Matsuzaki, R., Kawafune, K., Takahashi, T., Kawachi, M., Krientiz, L., Nozaki, H. 2015 Taxonomic study of Pyrobotrys (Spondylomoraceae, Chlorophyceae) based on comparative morphological and molecular analyses of culture strains established using novel methods. Cytologia, 80, 513-524.
Keywords: Molecular phylogeny; Morphology; Pyrobotrys; Pyrobotrys elongata; Spondylomoraceae; Stigma; Taxonomy; Ultrastructure
Strain(s): 3768376937703771377237733774 
DOI: 10.1508/cytologia.80.513

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