Strain number NIES-3388  
Phylum Chlorophyta  
Class Chlorophyceae  
Scientific name Colemanosphaera charkowiensis (Korshikov) Nozaki et al.  
Synonym Pandorina charkowiensis Korshikov  
Former name  
Common name Green alga  
Locality (Date of collection) Isanuma Pond Kawagoe Saitama Japan (2013-06-15)  
Latitude / Longitude 35.92138888 / 139.51444444 
Habitat (Isolation source) Freshwater (Ditch water)  
History < Nozaki, Hisayoshi  
Isolator (Date of isolation) Nozaki, Hisayoshi (2013-06-15)  
Identified by Nozaki, Hisayoshi  
State of strain Subculture; Unialgal; Clonal; Non-axenic  
Culture condition
(Preculture condition)
Medium:  AF-6VTAC  
Temperature:  20 C
Light intensity:  20 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  1 M  
Gene information 18S rRNA ( LC086350 ) , 18S-ITS1-5.8S-ITS2-28S ( AB905585 )  
Cell size (min - max)  
Organization  
Characteristics Planktonic ; Dioecious ; Anisogamous ; Resting spore-forming ; Authentic strain, Epitype (Nozaki, H. et al. 2014)  
Other strain no. Other strain no. : 2013-0615-IC-7  
Remarks  
Movie  
Reference
Nakada, T., Ito, T., Tomita, M. 2016 18S ribosomal RNA gene phylogeny of a colonial volvocalean lineage (Tetrabanaceae-Goniaceae-Volvocaceae, Volvocales, Chlorophyceae) and its close relatives. J. Jpn. Bot., 91, 345-354.
Keywords: 18S rRNA; colonial; green algae; molecular phylogeny; unicellular; Volvocales
Strain(s): 418456460474541544545564566571654666721725728735737867877879880881882883136222422246226625673388 

Nozaki, H., Yamada, K. T., Takahashi, F., Matsuzaki, R., Nakada, T. 2014 New "missing link" genus of the colonial volvocine green algae gives insights into the evolution of oogamy. BMC Evol. Biol., 14, 37 (article ID).
Strain(s): 3382338333843385338633873388 
DOI: 10.1186/1471-2148-14-37

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