Strain number NIES-2280  
Phylum Chlorophyta  
Class Chlorophyceae  
Scientific name Tetradesmus obliquus (Turpin) Kützing  
Former name Scenedesmus obliquus (Turpin) Kützing  
Common name Green alga  
Locality (Date of collection)  
Latitude / Longitude  
Habitat (Isolation source)  
History < IAM (2007) < Tsuzuki, Mikio (1988) < Oh-Hama, Takeshi < Senger, H. (1965) < Bishop, N. < Gaffron, H.  
Isolator (Date of isolation) Gaffron, H.  
Identified by  
State of strain Cryopreservation; Unialgal; Clonal; Axenic[2013 Nov]  
Culture condition
(Preculture condition)
Medium:  C (agar)  
Temperature:  20 C
Light intensity:  3-5 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  4 M  
Gene information  
Cell size (min - max) 5 - 10 μm  
Characteristics Starch production (Miyachi, S. et al. 1986)  
Other strain no. Other collection strain no. : IAM C-538
Other strain no. : Gaffron D-3  
Remarks Cryopreserved[2017 Nov]; Axenic 
Shen, X.-F., Liu, J.-J., Chu, F.-F., Lam, P.K.S., Zeng, R.J. 2015 Enhancement of FAME productivity of Scenedesmus obliquus by combining nitrogen deficiency with sufficient phosphorus supply in heterotrophic cultivation. Appl. Energy, 158, 348-354.
Keywords: Scenedesmus obliquus; Heterotrophic cultivation; Acetate; Nitrogen deficiency; Phosphorus; Biodiesel productivity
Strain(s): 2280 
DOI: 10.1016/j.apenergy.2015.08.057

Shimonaga, T., Konishi, M., Oyama, Y., Fujiwara, S., Satoh, A., Fujita, N., Colleoni, C., Buléon, A., Putaux, J.-L., Ball, S. G., Yokoyma, A., Hara, Y., Nakamura, Y., Tsuzuki, M. 2008 Variation in Storage α-polyglucans of the Porphyridiales (Rhodophyta). Plant Cell Physiol., 49, 103-116.
Keywords: Amylose; Floridean starch; Glycogen; Porphyridiales; Rhodophyta; Semi-amylopection
Strain(s): 181445305512138216022582280 
PubMed: 18079144
DOI: 10.1093/pcp/pcm172

Kotzabasis, K., Schuering, M., Senger, H. 1989 Occurrence of protochlorophyll and its phototransformation to chlorophyll in mutant C-2A' of Scenedesmus obliquus. Physiol. Plant., 75, 221-226.
Keywords: green algae; phototransformation; protochlorophyllide; protochlorophyllide reductase; Scenedesmus obliquus
Strain(s): 2280 
DOI: 10.1111/j.1399-3054.1989.tb06172.x

Miyachi, S., Tsuzuki, M., Maruyama, I., Ganter, M., Miyachi, S. 1986 Effects of CO2 concentration during growth on the intracellular structure of Chlorella and Scenedesmus (Chlorophyta). J. Phycol., 22, 313-319.
Keywords: Chlorella; CO2 concentration; electron microscopy; pyrenoid starch; Scenedesmus
Strain(s): 68721572160217021732280 

Oh-hama, T., Siebelt, F., Furihata, K., Seto, H., Miyachi, S., Ohmori, K. 1986 31P-NMR studies on inorganic polyphosphates in microalgae. J. Phycol., 22, 485-490.
Keywords: Anabaena cylindrica; Chlorella vulgaris 11h; inorganic polyphosphate; 13P nuclear magnetic resonance of algae
Strain(s): 2280 
DOI: 10.1111/j.1529-8817.1986.tb02492.x

Oh-hama, T., Seto, H., Miyachi, S. 1985 13C-Nuclear magnetic resonance studies of the biosynthesis of 5-aminolevulinic acid destined for chlorophyll formation in dark-grown Scenedesmus obliquus. Plant Sci., 42, 153-158.
Keywords: biosynthesis of 5-aminolevulinic acid; chlorophyll biosynthesis; 13C-nuclear magnetic resonance; Scenedesmus obliquus
Strain(s): 2280 
DOI: 10.1016/0168-9452(85)90121-9

Tsuzuki, M., Shimamoto, T., Yang, S. Y., Miyachi, S. 1984 Diversity in intracellular locality, nature, and function of carbonic anhydrase in verious plants. Ann. NY Acad. Sci., 429, 238-240.
Strain(s): 6872160216121622170217322352280 
DOI: 10.1111/j.1749-6632.1984.tb12341.x

Oh-hama, T., Seto, H., Otake, N., Miyachi, S. 1983 13C-NMR spectra of chlorophylls a and b in green algae biosynthetically enriched with [1-13C] glutamate. FEBS Lett., 153, 404-408.
Keywords: 5-aminolevulinic acid; chlorophyll a; chlorophyll b; 13C-NMR; [1-13C]glutamic acid; Scenedesmus obliquus
Strain(s): 2280 

Oh-hama, T., Seto, H., Otake, N., Miyachi, S. 1982 13C-NMR evidence for the pathway of chlorophyll biosynthesis in algae. Biochem. Biophys. Res. Commun., 105, 647-652.
Strain(s): 2280 

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