Strain number NIES-2212  
Phylum Chlorophyta  
Class Chlorophyceae  
Scientific name Chlamydomonas debaryana Goroschankin  
Synonym  
Former name Chlamydomonas angulosa Dill  
Common name Green alga  
Locality (Date of collection) Kobe Hyogo Japan  
Latitude / Longitude 34.689404 / 135.192204 
Habitat (Isolation source)  
History < IAM (2007) < UTEX (1996);  
Isolator (Date of isolation) Tsubo, Yoshihiro  
Identified by  
State of strain Subculture; Unialgal; Clonal; Axenic[2018 Feb]  
Culture condition
(Preculture condition)
Medium:  C (agar)  
Temperature:  20 C
Light intensity:  3-5 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  6 M  
Gene information Whole-genome ( BDDB00000000 ) , 18S rRNA ( AB701542 )  
Cell size (min - max) 7 - 12 μm  
Organization Unicellular; Flagellate 
Characteristics Phototaxis ; Starch production (Toyoshima et al. 2015) ; Genome decoded strain (Hirashima et al. 2016)  
Other strain no. Other collection strain no. : IAM C-549 (=C-1); Tsubo I16; UTEX 618; CCAP 11/59; SAG 4.72
 
Remarks Axenic 
Movie  
Reference
Hirashima, T., Toyoshima, M., Moriyama, T., Sato, N. 2018 Evolution of the phosphatidylcholine biosynthesis pathways in green algae: Combinatorial diversity of methyltransferases. J. Mol. Evol., 86, 68-76.
Keywords: Chlamydomonas; Metabolic diversity; Phosphatidylcholine biosynthesis; Phosphoethanolamine-N-methyltransferase; Phospholipid-N-methyltransferase
Strain(s): 2202220722122242 
PubMed: 29330556
DOI: 10.1007/s00239-017-9826-4

Toyoshima, M., Sato, N. 2018 Optimization of triacylglycerol and starch production in Chlamydomonas debaryana NIES-2212 with regard to light intensity and CO2 concentration. Microbiology, 164, 359-368.
Keywords: light intensity; CO2 supplement; TAG; Chlamydomonas debaryana; starch
Strain(s): 2212 
PubMed: 29458672
DOI: 10.1099/mic.0.000603

Widyaningrum, D., Iida, D., Tanabe, Y., Hayashi, Y., Kurniasih, S. D., Ohama, T. 2018 Acutely induced cell mortality in the unicellular green alga Chlamydomonas reinhardtii (Chlorophyceae) following exposure to acrylic resin nanoparticles. J. Phycol.,
Strain(s): 3514436241842143243845654557173273785810221048184821502169220122022208220922122280235224622578 
DOI: 10.1111/jpy.12798

Hirashima, T., Toyoshima, M., Moriyama, T., Nakamura, Y., Sato, N. 2017 Characterization of phosphoethanolamine-N-methyltransferases in green algae. Biochem. Biophys. Res. Commun., 488, 141-146.
Keywords: Green algal evolution; Chlamydomonas; Phosphatidylcholine biosynthesis; Phosphoethanolamine-N-methyltransferase
Strain(s): 22022207221222352242 
PubMed: 2848352
DOI: 10.1016/j.bbrc.2017.05.026

Hirashima, T., Tajima, N., Sato, N. 2016 Draft genome sequences of four species of Chlamydomonas containing phosphatidylcholine. GenomeA, 4, e01070-16 (article ID).
Strain(s): 2202220722122242 
PubMed: 27688324
DOI: 10.1128/genomeA.01070-16

Takahashi, K., Moestrup, Ø., Jordan, R. W., Iwataki, M. 2015 High-level accumulation of triacylglycerol and starch in photoautotrophically grown Chlamydomonas debaryana NIES-2212. Plant Cell Physiol., 56, 2447-2456.
Keywords: Biofuel; Chlamydomonas debaryana NIES-2212; Fatty acid; Starch; Triacylglycerol
Strain(s): 2212 
PubMed: 26542110
DOI: 10.1093/pcp/pcv163

Sakurai, K., Mori, N., Sato, N. 2014 Detection and characterization of phosphatidylcholine in various strains of the genus Chlamydomonas (Volvocales, Chlorophyceae). J. Plant Res., 127, 641-650.
Keywords: Chlamydomonas; Delta 5 desaturase; Diacylglyceryl-N,N,N-trimethylhomoserine; Fatty acid; Phosphatidylcholine
Strain(s): 22022207221222152219222522302242231624622499 
PubMed: 24947506
DOI: 10.1007/s10265-014-0644-0

Yumoto, K., Kasai, F., Kawachi, M. 2013 Taxonomic re-examination of Chlamydomonas strains maintained in the NIES-Collection. Microbiol. Cult. Coll., 29, 1-12.
Strain(s): 1584374384408849681021102210481733184818492201220222032204220622072208220922102211221222132214221522162218221922202222222322242225222822292230223122332234223522362237223922402241224222432246224722482314231523162317231823192320232123222323232424622463249925752576257725782579 

Nozaki, H., Mori, T., Misumi, O., Matsunaga, S., Kuroiwa, T. 2006 Males evolved from the dominant isogametic mating type. Curr. Biol., 16, R1018-R1020.
Strain(s): 16111612161316141615221222132214 
PubMed: 17174904
DOI: 10.1016/j.cub.2006.11.019

Sakaguchi, T., Tsuji, T., Nakajima, A., Horikoshi, T. 1979 Accumulation of cadmium by green microalgae. Eur. J. Appl. Microbiol. Biotechnol., 8, 207-215.
Strain(s): 2212227322742279 
DOI: 10.1007/BF00506184

Nakajima, A., Horikoshi, T., Sakaguchi, T. 1979 Uptake of copper ion by green microalgae. Agric. Biol. Chem., 43, 1455-1460.
Strain(s): 2212227322742279 
DOI: 10.1080/00021369.1979.10863659

Sakaguchi, T., Horikoshi, T., Nakajima, A. 1978 Uptake of uranium from seawater by microalgae. J. Ferm. Technol., 56, 561-565.
Strain(s): 2138213922122279 

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