Strain number NIES-2212  
Phylum Chlorophyta  
Class Chlorophyceae  
Scientific name Chlamydomonas debaryana Goroschankin  
Synonym  
Former name Chlamydomonas angulosa Dill  
Common name Green alga  
Locality (Date of collection) Kobe Hyogo Japan  
Latitude / Longitude 34.689404 / 135.192204 
Habitat (Isolation source)  
History < IAM (2007) < UTEX (1996);  
Isolator (Date of isolation) Tsubo, Yoshihiro  
Identified by  
State of strain Subculture; Unialgal; Clonal; Axenic[2013 Feb]  
Culture condition
(Preculture condition)
Medium:  C (agar)  
Temperature:  20 C
Light intensity:  3-5 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  6 M  
Gene information Whole-genome ( BDDB00000000 ) , 18S rRNA ( AB701542 )  
Cell size (min - max) 7 - 12 μm  
Organization Unicellular; Flagellate 
Characteristics Phototaxis ; Starch production (Toyoshima, M. et al. 2015) ; Genome decoded strain (Hirashima, T. et al. 2016)  
Other strain no. Other collection strain no. : IAM C-549 (=C-1); Tsubo I16; UTEX 618; CCAP 11/59; SAG 4.72
 
Remarks Axenic 
Movie  
Reference
Hirashima, T., Toyoshima, M., Moriyama, T., Nakamura, Y., Sato, N. 2017 Characterization of phosphoethanolamine-N-methyltransferases in green algae. Biochem. Biophys. Res. Commun., 488, 141-146.
Keywords: Green algal evolution; Chlamydomonas; Phosphatidylcholine biosynthesis; Phosphoethanolamine-N-methyltransferase
Strain(s): 22022207221222352242 
PubMed: 2848352
DOI: 10.1016/j.bbrc.2017.05.026

Hirashima, T., Tajima, N., Sato, N. 2016 Draft genome sequences of four species of Chlamydomonas containing phosphatidylcholine. GenomeA, 4, e01070-16 (article ID).
Strain(s): 2202220722122242 
DOI: 10.1128/genomeA.01070-16

Takahashi, K., Moestrup, Ø., Jordan, R. W., Iwataki, M. 2015 High-level accumulation of triacylglycerol and starch in photoautotrophically grown Chlamydomonas debaryana NIES-2212. Plant Cell Physiol., 56, 2447-2456.
Keywords: Biofuel; Chlamydomonas debaryana NIES-2212; Fatty acid; Starch; Triacylglycerol
Strain(s): 2212 
PubMed: 26542110
DOI: 10.1093/pcp/pcv163

Sakurai, K., Mori, N., Sato, N. 2014 Detection and characterization of phosphatidylcholine in various strains of the genus Chlamydomonas (Volvocales, Chlorophyceae). J. Plant Res., 127, 641-650.
Keywords: Chlamydomonas; Delta 5 desaturase; Diacylglyceryl-N,N,N-trimethylhomoserine; Fatty acid; Phosphatidylcholine
Strain(s): 22022207221222152219222522302242231624622499 
DOI: 10.1007/s10265-014-0644-0

Yumoto, K., Kasai, F., Kawachi, M. 2013 Taxonomic re-examination of Chlamydomonas strains maintained in the NIES-Collection. Microbiol. Cult. Coll., 29, 1-12.
Strain(s): 1584374384408849681021102210481733184818492201220222032204220622072208220922102211221222132214221522162218221922202222222322242225222822292230223122332234223522362237223922402241224222432246224722482314231523162317231823192320232123222323232424622463249925752576257725782579 

Nozaki, H., Mori, T., Misumi, O., Matsunaga, S., Kuroiwa, T. 2006 Males evolved from the dominant isogametic mating type. Curr. Biol., 16, R1018-R1020.
Strain(s): 16111612161316141615221222132214 
PubMed: 17174904
DOI: 10.1016/j.cub.2006.11.019

Sakaguchi, T., Tsuji, T., Nakajima, A., Horikoshi, T. 1979 Accumulation of cadmium by green microalgae. Eur. J. Appl. Microbiol. Biotechnol., 8, 207-215.
Strain(s): 2212227322742279 
DOI: 10.1007/BF00506184

Nakajima, A., Horikoshi, T., Sakaguchi, T. 1979 Uptake of copper ion by green microalgae. Agric. Biol. Chem., 43, 1455-1460.
Strain(s): 2212227322742279 
DOI: 10.1080/00021369.1979.10863659

Sakaguchi, T., Horikoshi, T., Nakajima, A. 1978 Uptake of uranium from seawater by microalgae. J. Ferm. Technol., 56, 561-565.
Strain(s): 2138213922122279 

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