Strain number NIES-2202  
Phylum Chlorophyta  
Class Chlorophyceae  
Scientific name Chlamydomonas applanata Pringsheim  
Synonym  
Former name Chlamydomonas aggregata Deason et Bold  
Common name Green alga  
Locality (Date of collection) Williamson County Texas USA  
Latitude / Longitude 30.689888 /  
Habitat (Isolation source) Terrestrial  
History < IAM (2007) < BIU (UTEX; 1967);  
Isolator (Date of isolation) Deason, T. R. (1957-**-**)  
Identified by  
State of strain Cryopreservation; Unialgal; Clonal; Axenic[2018 Jan]  
Culture condition
(Preculture condition)
Medium:  C (agar)  
Temperature:  20 C
Light intensity:  3-5 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  6 M  
Gene information Whole-genome ( BDCZ00000000 ) , 18S rRNA ( AB701514 )  
Cell size (min - max) 2 - 10 μm  
Organization Unicellular; Flagellate 
Characteristics Phototaxis ; Genome decoded strain (Hirashima et al. 2016)  
Other strain no. Other collection strain no. : IAM C-214; SAG 2.72; UTEX 969
Other strain no. : Deason T-1-12  
Remarks Cryopreserved; Axenic 
Movie  
Reference
Hirashima, T., Toyoshima, M., Moriyama, T., Sato, N. 2018 Evolution of the phosphatidylcholine biosynthesis pathways in green algae: Combinatorial diversity of methyltransferases. J. Mol. Evol., 86, 68-76.
Keywords: Chlamydomonas; Metabolic diversity; Phosphatidylcholine biosynthesis; Phosphoethanolamine-N-methyltransferase; Phospholipid-N-methyltransferase
Strain(s): 2202220722122242 
PubMed: 29330556
DOI: 10.1007/s00239-017-9826-4

Widyaningrum, D., Iida, D., Tanabe, Y., Hayashi, Y., Kurniasih, S. D., Ohama, T. 2018 Acutely induced cell mortality in the unicellular green alga Chlamydomonas reinhardtii (Chlorophyceae) following exposure to acrylic resin nanoparticles. J. Phycol.,
Strain(s): 3514436241842143243845654557173273785810221048184821502169220122022208220922122280235224622578 
DOI: 10.1111/jpy.12798

Hirashima, T., Toyoshima, M., Moriyama, T., Nakamura, Y., Sato, N. 2017 Characterization of phosphoethanolamine-N-methyltransferases in green algae. Biochem. Biophys. Res. Commun., 488, 141-146.
Keywords: Green algal evolution; Chlamydomonas; Phosphatidylcholine biosynthesis; Phosphoethanolamine-N-methyltransferase
Strain(s): 22022207221222352242 
PubMed: 2848352
DOI: 10.1016/j.bbrc.2017.05.026

Hirashima, T., Tajima, N., Sato, N. 2016 Draft genome sequences of four species of Chlamydomonas containing phosphatidylcholine. GenomeA, 4, e01070-16 (article ID).
Strain(s): 2202220722122242 
PubMed: 27688324
DOI: 10.1128/genomeA.01070-16

Sakurai, K., Mori, N., Sato, N. 2014 Detection and characterization of phosphatidylcholine in various strains of the genus Chlamydomonas (Volvocales, Chlorophyceae). J. Plant Res., 127, 641-650.
Keywords: Chlamydomonas; Delta 5 desaturase; Diacylglyceryl-N,N,N-trimethylhomoserine; Fatty acid; Phosphatidylcholine
Strain(s): 22022207221222152219222522302242231624622499 
PubMed: 24947506
DOI: 10.1007/s10265-014-0644-0

Yumoto, K., Kasai, F., Kawachi, M. 2013 Taxonomic re-examination of Chlamydomonas strains maintained in the NIES-Collection. Microbiol. Cult. Coll., 29, 1-12.
Strain(s): 158437438440884968102110221048173318481849220122022203220422062207220822092210221122122213221422152216221822192220222222232224222522282229223022312233223422352236223722382239224022412242224322462247224823142315231623172318231923202321232223232324246224632499257525762577257825793284 

Brand, J. J., Wright, J. N. 1989 Hydrogen production by eukaryotic algae. Biotech. Bioeng., 33, 1482-1488.
Strain(s): 2151216921902194220122022203220422112213221922792291 

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