Strain number NIES-2152  
Phylum Chlorophyta  
Class Trebouxiophyceae  
Scientific name Parachlorella kessleri (Fott & Nováková) Krienitz, Hegewald, Hepperle, Huss, Rohr & Wolf  
Synonym Chlorella kessleri Fott & Nováková  
Former name  
Common name  
Locality (Date of collection)  
Latitude / Longitude  
Habitat (Isolation source)  
History < IAM (2007) < Yakult < IAM;  
Isolator (Date of isolation) Watanabe, Atsushi  
Identified by Kessler, E. (1994); Confirmed at NIES by DNA sequencing  
State of strain Cryopreservation; Unialgal; Clonal; Axenic[2008 Apr]  
Culture condition
(Preculture condition)
Medium:  C (agar)  
Temperature:  20 C
Light intensity:  8-15 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  3 M  
Gene information Whole-genome ( BBXU00000000 ) , 18S rRNA ( AB488589 )  
Cell size (min - max)  
Organization Unicellular 
Characteristics Starch production (Takeshita, T. et al. 2014) ; Genome decoded strain (Ota, S. et al. 2016)  
Other strain no. Other collection strain no. : IAM C-33
 
Remarks Cryopreserved 
Movie  
Reference
Ota, S., Oshima, K., Yamazaki, T., Kim, S., Yu, Z., Yoshihara, M., Takeda, K., Takeshita, T., Hirata, A., Bišová, K., Zachleder, V., Hattori, M., Kawano, S. 2016 Highly efficient lipid production in the green alga Parachlorella kessleri: draft genome and transcriptome endorsed by whole-cell 3D ultrastructure. Biotechnol. Biofuels, 9, 13 (article ID).
Keywords: Genome; Green alga; Lipid body; Parachlorella kessleri; RNA-seq; Whole-genome sequence; Transcriptome; 3D-TEM
Strain(s): 2152 
PubMed: 26811738
DOI: 10.1186/s13068-016-0424-2

Ota, S., Yoshihara, M., Yamazaki, T., Takeshita, T., Hirata, A., Konomi, M., Oshima, K., Hattori, M., Bišová, K., Zachleder, V., Kawano, S. 2016 Deciphering the relationship among phosphate dynamics, electron-dense body and lipid accumulation in the green alga Parachlorella kessleri. Sci Rep, 6, 25731 (article ID).
Strain(s): 2152 
DOI: 10.1038/srep25731

Takeshita, T., Ota, S., Yamazaki, T., Hirata, A., Zachleder, V., Kawano, S. 2014 Starch and lipid accumulation in eight strains of six Chlorella species under comparatively high light intensity and aeration culture conditions. Biores. Technol., 158, 127-134.
Keywords: Accumulation shift; Lipid; Chlorella; Starch; Strong light
Strain(s): 215121522159216921702179 
DOI: 10.1016/j.biortech.2014.01.135

Yamazaki, T; Hirai, C; Ota, S; Kuwano, K; Kawano, S. 2014 Use of FM1-43, a membrane-specific fluorescent dye, to estimate plasma membrane integrity in the cryopreservation of green algae. CryoLetters, 35, 180-187.
Keywords: FM1-43, cryopreservation, two-step freezing, plasma membrane, green algae, Parachlorella kessleri
Strain(s): 2152 

Ota, S., Matsuda, T., Takeshita, T., Yamazaki, T., Kazama, Y., Abe, T., Kawano, S. 2013 Phenotypic spectrum of Parachlorella kessleri (Chlorophyta) mutants produced by heavy-ion irradiation. Biores. Technol., 149, 432-438.
Keywords: Algae; Breeding; Parachlorella; Heavy-ion irradiation; Mutation
Strain(s): 2152 
PubMed: 24135567
DOI: 10.1016/j.biortech.2013.09.079

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