Strain Data

Strain number		NIES-574
Phylum		Chlorophyta
Class		Chlorophyceae
Scientific name		Pandorina morum (O.F.Müller) Bory var. morum
Synonym		Volvox morum O.F.Müller 1786
Former name
Common name		Green alga
Locality (Date of collection)		Nepal (1986-09-**)
Latitude / Longitude
Habitat (Isolation source)		Freshwater (Paddy soil)
History		< Nozaki, Hisayoshi
Isolator (Date of isolation)		Nozaki, Hisayoshi (1987-09-16)
Identified by		Nozaki, Hisayoshi
State of strain		Cryopreservation; Unialgal; Clonal; Axenic[2018 Feb]
Culture condition (Preculture condition)		Medium: VT Temperature: 20 C Light intensity: 7-10 µmol photons/m²/sec, L/D cycle: 10L:14D Duration: 75 D
Gene information		atpB ( AB014025 AB014026 ) , rbcL ( D63442 ) , psaA ( AB044226 ) , psaB ( AB044452 ) , psbC ( AB044505 )
Cell size (min - max)
Organization		Colonial; Flagellate
Characteristics		Phototaxis ; Heterothallic ; Isogamous ; Mating type (+) ; Crosses with NIES-575
Other strain no.		Other strain no. : 7916-P-7
Remarks		Cryopreserved; Axenic
Movie

Reference
Yamagishi, S., Yamamoto, K., Takahashi, K., Kawai-Toyooka, H., Suzuki, S., Matsuzaki, R., Yamaguchi, H., Kawachi, M., Higashiyama, T., Nozaki, H. 2023 Evolutionary analysis of MID homologs during the transition from homothallic species to heterothallic species in Volvox sect. Volvox (Chlorophyceae). Phycol. Res., 72, 46-55. Keywords: male gamete; mating system; minus dominance gene (MID); molecular evolution; sex; sperm packet; volvocine green algae Strain(s): 289, 456, 460, 541, 544, 545, 569, 572, 574, 582, 652, 653, 654, 732, 851, 852, 857, 872, 891, 892, 1362, 1363, 1708, 1859, 2457, 2569, 2735, 2736, 2740, 3382, 3383, 3780, 3782, 3786, 3874, 3984, 3986, 3988, 4021, 4025, 4336, 4432, 4535, 4536, 4537 DOI: 10.1111/pre.12538 Hanschen, E. R., Herron, M. D., Wiens, J. J., Nozaki, H., Michod, R. E. 2018 Repeated evolution and reversibilityof self-fertilization in the volvocinegreen algae. Evolution, 72, 386-398. Keywords: Haploid; phylogenetics; self-fertilization; sex; sexual reproduction; volvocine green algae Strain(s): 456, 458, 460, 541, 544, 545, 564, 566, 568, 569, 572, 574, 578, 582, 653, 732, 851, 856, 857, 872, 875, 876, 877, 878, 880, 883, 891, 892, 1362, 2457, 2569, 3382, 3780, 3782 PubMed: 29134623 DOI: 10.1111/evo.13394 Hanschen, E. R., Davison, D. R., Ferris, P. J., Michod, R. E. 2018 On the rediscovery of Volvox perglobator (Volvocales, Chlorophyceae) and the evolution of outcrossing from self-fertilization. Evol. Ecol. Res., 19, 299-318. Keywords: homothallism; phylogenetics; reversion; self-fertilization; sexual reproduction; volvocine green algae Strain(s): 289, 421, 456, 458, 460, 474, 522, 541, 543, 544, 545, 564, 566, 568, 569, 572, 574, 578, 582, 653, 732, 751, 851, 856, 872, 876, 877, 878, 880, 882, 1038, 1122, 1140, 1148, 1156, 1157, 1362, 1708, 1711, 1713, 1859, 2457, 2569, 2738, 3382, 3388, 3780, 3782, 3986, 4145, 4259, 4260 Nakazawa, A. & Nishii, I. 2012 Amidic and acetonic cryoprotectants improve cryopreservation of volvocine green algae. CryoLetters, 33, 201-212. Keywords: cryopreservation; dimethylformamide; hydroxyacetone; Volvocaceae; Volvox; Chlamydomonas Strain(s): 545, 564, 574, 717, 729, 730, 731, 1361, 2236 PubMed: 22825787 Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55. Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection Strain(s): 19, 21, 22, 23, 25, 26, 27, 29, 31, 32, 34, 35, 36, 37, 38, 40, 44, 45, 46, 50, 51, 52, 53, 54, 55, 56, 58, 59, 60, 66, 67, 68, 73, 74, 75, 77, 78, 79, 80, 81, 87, 89, 92, 93, 95, 97, 98, 99, 100, 105, 106, 107, 108, 109, 110, 111, 112, 119, 120, 127, 128, 129, 130, 131, 132, 133, 134, 135, 137, 139, 144, 152, 153, 154, 155, 156, 157, 158, 159, 160, 162, 163, 164, 165, 166, 167, 168, 171, 177, 186, 189, 192, 193, 197, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 214, 215, 216, 217, 224, 226, 230, 231, 232, 242, 243, 244, 245, 246, 248, 259, 263, 266, 267, 268, 287, 288, 290, 294, 295, 297, 299, 300, 302, 303, 305, 306, 308, 309, 310, 312, 313, 329, 334, 337, 338, 340, 341, 342, 349, 351, 359, 361, 362, 375, 378, 379, 380, 382, 384, 385, 390, 394, 396, 398, 415, 416, 419, 422, 423, 426, 427, 429, 430, 431, 433, 434, 436, 438, 439, 440, 446, 447, 448, 450, 451, 452, 453, 454, 455, 456, 458, 460, 464, 474, 478, 479, 481, 503, 504, 505, 506, 507, 509, 510, 512, 514, 515, 522, 523, 524, 527, 529, 530, 531, 532, 536, 537, 538, 539, 540, 541, 542, 544, 545, 546, 564, 565, 566, 568, 569, 570, 572, 573, 574, 575, 578, 579, 580, 581, 582, 584, 592, 593, 594, 595, 596, 597, 598, 610, 628, 630, 631, 635, 638, 639, 640, 641, 642, 643, 644, 645, 647, 648, 649, 650, 652, 653, 655, 657, 658, 659, 660, 661, 662, 663, 664, 665, 666, 667, 672, 685, 686, 687, 688, 689, 691, 692, 693, 694, 717, 718, 719, 722, 723, 726, 727, 729 Nozaki, H., Onishi, K., Morita, E. 2002 Differences in pyrenoid morphology are correlated with differences in the rbcL genes of members of the Chloromonas lineage (Volvocales, Chlorophyceae). J. Mol. Evol., 55, 414-430. Keywords: ATP synthase β-subunit (atpB) gene; Chloromonas; gene evolution; large subunit of Rubisco (rbcL) gene; morphological evolution; P700 chlorophyll a-apoprotein A2 (psaB) gene; pyrenoid; Volvocales Strain(s): 144, 439, 446, 447, 456, 474, 522, 564, 566, 569, 574, 582, 653, 732, 851, 858, 861, 875, 883 PubMed: 12355262 DOI: 10.1007/s00239-002-2338-9 Nozaki, H., Misawa, K., Kajita, T., Kato, M., Nohara, S., Watanabe, M. M. 2000 Origin and evolution of the colonial Volvocales (Chlorophyceae) as inferred from multiple, chloroplast gene sequences. Mol. Phylogenet. Evol., 17, 256-268. Strain(s): 456, 460, 474, 541, 545, 564, 566, 569, 572, 574, 582, 653, 666, 722, 727, 730, 731, 732, 736, 851, 854, 872, 884, 886, 888, 889, 890, 893, 896 PubMed: 11083939 DOI: 10.1006/mpev.2000.0831 Nozaki, H., Ohta, N., Takano, H., Watanabe, M. M. 1999 Reexamination of phylogenetic relationships within the colonial Volvocales (Chlorophyta): an analysis of atpB and rbcL gene sequences. J. Phycol., 35, 104-112. Keywords: atpB gene; Chlorophyta; Gonium; molecular phylogeny; rbcL gene; Volvocaceae; volvocine series; Volvox Strain(s): 456, 460, 541, 564, 566, 568, 569, 572, 574, 582, 653, 666, 722, 727, 730, 731, 732, 736, 851, 852, 858, 861, 884 DOI: 10.1046/j.1529-8817.1999.3510104.x Nozaki, H., Ito, M., Sano, R., Uchida, H., Watanabe, M. M., Kuroiwa, T. 1995 Phylogenetic relationships within the colonial Volvocales (Chlorophyta) inferred from rbcL gene sequence data. J. Phycol., 31, 970-979. Keywords: Chlorophyta; Goniaceae; large subunit of ribulose-1,5-bisphosphate carboxylase / oxygenase (rbcL) gene; molecular phylogeny; Tetrabaenaceae; Volvocaceae Strain(s): 456, 460, 541, 564, 566, 569, 572, 574, 653, 731, 732 DOI: 10.1111/j.0022-3646.1995.00970.x Nozaki, H. 1988 Colonial Volvocales (Chlorophyta) from Kathmandu, Nepal. In Cryptogams of the Himalayas. Vol. 1. The Kathmandu Valley, Eds. by Watanabe, M. & Malla, S. B., Department of Botany, National Science Museum, Tsukuba, pp. 39-46. Strain(s): 568, 574, 575

Reference

Yamagishi, S., Yamamoto, K., Takahashi, K., Kawai-Toyooka, H., Suzuki, S., Matsuzaki, R., Yamaguchi, H., Kawachi, M., Higashiyama, T., Nozaki, H. 2023 Evolutionary analysis of MID homologs during the transition from homothallic species to heterothallic species in Volvox sect. Volvox (Chlorophyceae). Phycol. Res., 72, 46-55.
Keywords: male gamete; mating system; minus dominance gene (MID); molecular evolution; sex; sperm packet; volvocine green algae
Strain(s): 289, 456, 460, 541, 544, 545, 569, 572, 574, 582, 652, 653, 654, 732, 851, 852, 857, 872, 891, 892, 1362, 1363, 1708, 1859, 2457, 2569, 2735, 2736, 2740, 3382, 3383, 3780, 3782, 3786, 3874, 3984, 3986, 3988, 4021, 4025, 4336, 4432, 4535, 4536, 4537
DOI: 10.1111/pre.12538

Hanschen, E. R., Herron, M. D., Wiens, J. J., Nozaki, H., Michod, R. E. 2018 Repeated evolution and reversibilityof self-fertilization in the volvocinegreen algae. Evolution, 72, 386-398.
Keywords: Haploid; phylogenetics; self-fertilization; sex; sexual reproduction; volvocine green algae
Strain(s): 456, 458, 460, 541, 544, 545, 564, 566, 568, 569, 572, 574, 578, 582, 653, 732, 851, 856, 857, 872, 875, 876, 877, 878, 880, 883, 891, 892, 1362, 2457, 2569, 3382, 3780, 3782
PubMed: 29134623
DOI: 10.1111/evo.13394

Hanschen, E. R., Davison, D. R., Ferris, P. J., Michod, R. E. 2018 On the rediscovery of Volvox perglobator (Volvocales, Chlorophyceae) and the evolution of outcrossing from self-fertilization. Evol. Ecol. Res., 19, 299-318.
Keywords: homothallism; phylogenetics; reversion; self-fertilization; sexual reproduction; volvocine green algae
Strain(s): 289, 421, 456, 458, 460, 474, 522, 541, 543, 544, 545, 564, 566, 568, 569, 572, 574, 578, 582, 653, 732, 751, 851, 856, 872, 876, 877, 878, 880, 882, 1038, 1122, 1140, 1148, 1156, 1157, 1362, 1708, 1711, 1713, 1859, 2457, 2569, 2738, 3382, 3388, 3780, 3782, 3986, 4145, 4259, 4260

Nakazawa, A. & Nishii, I. 2012 Amidic and acetonic cryoprotectants improve cryopreservation of volvocine green algae. CryoLetters, 33, 201-212.
Keywords: cryopreservation; dimethylformamide; hydroxyacetone; Volvocaceae; Volvox; Chlamydomonas
Strain(s): 545, 564, 574, 717, 729, 730, 731, 1361, 2236
PubMed: 22825787

Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55.
Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection
Strain(s): 19, 21, 22, 23, 25, 26, 27, 29, 31, 32, 34, 35, 36, 37, 38, 40, 44, 45, 46, 50, 51, 52, 53, 54, 55, 56, 58, 59, 60, 66, 67, 68, 73, 74, 75, 77, 78, 79, 80, 81, 87, 89, 92, 93, 95, 97, 98, 99, 100, 105, 106, 107, 108, 109, 110, 111, 112, 119, 120, 127, 128, 129, 130, 131, 132, 133, 134, 135, 137, 139, 144, 152, 153, 154, 155, 156, 157, 158, 159, 160, 162, 163, 164, 165, 166, 167, 168, 171, 177, 186, 189, 192, 193, 197, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 214, 215, 216, 217, 224, 226, 230, 231, 232, 242, 243, 244, 245, 246, 248, 259, 263, 266, 267, 268, 287, 288, 290, 294, 295, 297, 299, 300, 302, 303, 305, 306, 308, 309, 310, 312, 313, 329, 334, 337, 338, 340, 341, 342, 349, 351, 359, 361, 362, 375, 378, 379, 380, 382, 384, 385, 390, 394, 396, 398, 415, 416, 419, 422, 423, 426, 427, 429, 430, 431, 433, 434, 436, 438, 439, 440, 446, 447, 448, 450, 451, 452, 453, 454, 455, 456, 458, 460, 464, 474, 478, 479, 481, 503, 504, 505, 506, 507, 509, 510, 512, 514, 515, 522, 523, 524, 527, 529, 530, 531, 532, 536, 537, 538, 539, 540, 541, 542, 544, 545, 546, 564, 565, 566, 568, 569, 570, 572, 573, 574, 575, 578, 579, 580, 581, 582, 584, 592, 593, 594, 595, 596, 597, 598, 610, 628, 630, 631, 635, 638, 639, 640, 641, 642, 643, 644, 645, 647, 648, 649, 650, 652, 653, 655, 657, 658, 659, 660, 661, 662, 663, 664, 665, 666, 667, 672, 685, 686, 687, 688, 689, 691, 692, 693, 694, 717, 718, 719, 722, 723, 726, 727, 729

Nozaki, H., Onishi, K., Morita, E. 2002 Differences in pyrenoid morphology are correlated with differences in the rbcL genes of members of the Chloromonas lineage (Volvocales, Chlorophyceae). J. Mol. Evol., 55, 414-430.
Keywords: ATP synthase β-subunit (atpB) gene; Chloromonas; gene evolution; large subunit of Rubisco (rbcL) gene; morphological evolution; P700 chlorophyll a-apoprotein A2 (psaB) gene; pyrenoid; Volvocales
Strain(s): 144, 439, 446, 447, 456, 474, 522, 564, 566, 569, 574, 582, 653, 732, 851, 858, 861, 875, 883
PubMed: 12355262
DOI: 10.1007/s00239-002-2338-9

Nozaki, H., Misawa, K., Kajita, T., Kato, M., Nohara, S., Watanabe, M. M. 2000 Origin and evolution of the colonial Volvocales (Chlorophyceae) as inferred from multiple, chloroplast gene sequences. Mol. Phylogenet. Evol., 17, 256-268.
Strain(s): 456, 460, 474, 541, 545, 564, 566, 569, 572, 574, 582, 653, 666, 722, 727, 730, 731, 732, 736, 851, 854, 872, 884, 886, 888, 889, 890, 893, 896
PubMed: 11083939
DOI: 10.1006/mpev.2000.0831

Nozaki, H., Ohta, N., Takano, H., Watanabe, M. M. 1999 Reexamination of phylogenetic relationships within the colonial Volvocales (Chlorophyta): an analysis of atpB and rbcL gene sequences. J. Phycol., 35, 104-112.
Keywords: atpB gene; Chlorophyta; Gonium; molecular phylogeny; rbcL gene; Volvocaceae; volvocine series; Volvox
Strain(s): 456, 460, 541, 564, 566, 568, 569, 572, 574, 582, 653, 666, 722, 727, 730, 731, 732, 736, 851, 852, 858, 861, 884
DOI: 10.1046/j.1529-8817.1999.3510104.x

Nozaki, H., Ito, M., Sano, R., Uchida, H., Watanabe, M. M., Kuroiwa, T. 1995 Phylogenetic relationships within the colonial Volvocales (Chlorophyta) inferred from rbcL gene sequence data. J. Phycol., 31, 970-979.
Keywords: Chlorophyta; Goniaceae; large subunit of ribulose-1,5-bisphosphate carboxylase / oxygenase (rbcL) gene; molecular phylogeny; Tetrabaenaceae; Volvocaceae
Strain(s): 456, 460, 541, 564, 566, 569, 572, 574, 653, 731, 732
DOI: 10.1111/j.0022-3646.1995.00970.x

Nozaki, H. 1988 Colonial Volvocales (Chlorophyta) from Kathmandu, Nepal. In Cryptogams of the Himalayas. Vol. 1. The Kathmandu Valley, Eds. by Watanabe, M. & Malla, S. B., Department of Botany, National Science Museum, Tsukuba, pp. 39-46.
Strain(s): 568, 574, 575

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