保存株情報

株番号		NIES-123
門名		Chlorophyta
綱名		Chlorophyceae
学名		Rusalka fusiformis (Matvienko) Nakada
シノニム		Chlorogonium fusiforme Matvienko
以前使われていた種名		Chlorogonium metamorphum Skuja (in IAM)
一般名		緑藻
地名（採集年月）		日本北海道ニセコ町神仙沼（1964-07-30）
緯度/経度		42.904268 / 140.592828
生息環境（分離源）		淡水
分離後の履歴		← IAM;
分離者（分離年月）		市村輝宜 (1964-07-30)
同定者		市村輝宜; 野崎久義; 仲田崇志（再同定）
株の状態		継代培養; 無菌[2017 Dec]; 単藻; クローン
培養条件（前培養条件）		培地名： AF-6 温度： 20 (25) ℃ 光強度： 2 (14-16) µmol photons/m²/sec, 明暗周期： 10L:14D 継代培養周期： 2 M (14 D)
遺伝子情報		18S rRNA ( AB360750 ) , atpB ( AB084329 ) , rbcL ( AB010242 ) , psaB ( AB084370 )
特性		Authentic株 ; タイプ標本 (NIES-50007, Epitype) ; 雌雄同株
サイズ (min - max)		- 18 μm
体制		単細胞性; 鞭毛性
その他の株番号		他のコレクション番号：IAM C-349 (=C-569) その他の株番号：MKF-14
注意事項		無菌
動画

文献
Munakata, H., Nakada, T., Nakahigashi, K., Nozaki, H., Tomita, M. 2016 Phylogenetic position and molecular chronology of a colonial green flagellate, Stephanosphaera pluvialis (Volvocales, Chlorophyceae), among unicellular algae. J. Eucaryot. Microbiol., 63, 340-348. キーワード: Balticola; coloniality; divergence time; evolution; molecular phylogeny Strain(s): 123, 144, 421, 428, 432, 439, 446, 567, 569, 571, 656, 861, 887, 968, 1048, 2293, 2564, 4124, 4125 PubMed: 26595722 DOI: 10.1111/jeu.12283 Nakada, T., Nozaki, H., Pröschold, T. 2008 Molecular phylogeny, ultrastructure, and taxonomic revision of Chlorogonium (Chlorophyta): emendation of Chlorogonium and description of Gungnir gen. nov. and Rusalka gen.nov. J. Phycol., 44, 751-760. キーワード: Chlorogonium; Gungnir gen.nov.; molecular phylogeny; morphology; Rusalka gen.nov.; taxonomy; ultrastructure; Volvocales Strain(s): 123, 439, 692, 751, 755, 761, 1357, 1359, 1360, 1869, 4125 DOI: 10.1111/j.1529-8817.2008.00525.x Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55. キーワード: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection Strain(s): 19, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 44, 45, 46, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 73, 74, 75, 76, 77, 78, 79, 80, 81, 87, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 119, 120, 122, 123, 124, 125, 127, 128, 129, 130, 131, 132, 133, 134, 135, 137, 138, 139, 144, 147, 148, 150, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 162, 163, 164, 165, 166, 167, 168, 169, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 185, 186, 187, 188, 189, 191, 192, 193, 194, 195, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 224, 226, 227, 228, 229, 230, 231, 232, 241, 242, 243, 244, 245, 246, 248, 257, 259, 261, 262, 263, 266, 267, 268, 271, 287, 288, 289, 290, 294, 295, 297, 298, 299, 300, 301, 302, 303, 305, 306, 308, 309, 310, 311, 312, 313, 329, 334, 336, 337, 338, 339, 340, 341, 342, 349, 351, 359, 360, 361, 362, 375, 378, 379, 380, 382, 384, 385, 390, 394, 396, 397, 398, 415, 416, 418, 419, 421, 422, 423, 424, 425, 426, 427, 428, 429, 430, 431, 432, 433, 434, 436, 437, 438, 439, 440, 446, 447, 448, 449, 450, 451, 452, 453, 454, 455, 456, 457, 458, 459, 460, 464, 468, 469, 474, 478, 479, 480, 481, 503, 504, 505, 506, 507, 509, 510, 512, 514, 515, 522, 523, 524, 527, 528, 529, 530, 531, 532, 536, 537, 538, 539, 540, 541, 542, 543, 544, 545, 546, 564, 565, 566, 567, 568, 569, 570, 571, 572, 573, 574, 575, 576, 577, 578, 579, 580, 581, 582, 583, 584, 585, 592, 593, 594, 595, 596, 597, 598, 604, 610, 628, 630, 631, 632, 633, 634, 635, 636, 637, 638, 639, 640, 641, 642, 643, 644, 645, 646, 647, 648, 649, 650, 651, 652, 653, 654, 655, 656, 657, 658, 659, 660, 661, 662, 663, 664, 665, 666, 667, 672, 685, 686, 687, 688, 689, 690, 691, 692, 693, 694, 717, 718, 719, 720, 721, 722, 723, 724, 725, 726, 727, 728, 729 Nozaki, H., Onishi, K., Morita, E. 2002 Differences in pyrenoid morphology are correlated with differences in the rbcL genes of members of the Chloromonas lineage (Volvocales, Chlorophyceae). J. Mol. Evol., 55, 414-430. キーワード: ATP synthase β-subunit (atpB) gene; Chloromonas; gene evolution; large subunit of Rubisco (rbcL) gene; morphological evolution; P700 chlorophyll a-apoprotein A2 (psaB) gene; pyrenoid; Volvocales Strain(s): 96, 123, 144, 213, 418, 421, 425, 428, 432, 439, 446, 447, 456, 474, 522, 564, 566, 567, 569, 571, 574, 582, 653, 732, 851, 858, 861, 875, 882, 883, 968 PubMed: 12355262 DOI: 10.1007/s00239-002-2338-9 Nozaki, H., Ohta, N., Morita, E., Watanabe, M. M. 1998 Toward a natural system of species in Chlorogonium (Volvocales, Chlorophyta): a combined analysis of morphological and rbcL gene sequence data. J. Phycol., 34, 1024-1037. キーワード: Chlorogonium; Chlorogonium kasakii sp. nov.; Chlorophyta; molecular phylogeny; monophyletic species; morphology; natural systematics; rbcL gene; species taxonomy; Volvocales Strain(s): 123, 439, 446, 692, 742, 743, 744, 745, 746, 747, 748, 749, 750, 751, 752, 753, 754, 755, 756, 757, 758, 759, 760, 761 DOI: 10.1046/j.1529-8817.1998.341024.x Nozaki, H., Aizawa, K., Watanabe, M. M. 1996 Re-examination of two NIES strains labeled Chlorogonium metamorphum (Volvocales, Chlorophyta) from Japan. Nova Hedwigia Beiheft, 112, 483-490. キーワード: Chlorogonium; Chlorophyta; morphology; neotype strain; species identification; Volvocales Strain(s): 123, 446 Ichimura, T., Itoh, T. 1977 17. Preservation methods of microalgae (I) [17. Bisaisôrui no hozonhô (I)]. In Preservation methods of microorganisms [Biseibutsu Hozonhô], Ed. by Nei, T., University of Tokyo Press, Tokyo, p. 355-373 (in Japanese without English title). Strain(s): 19, 21, 22, 23, 24, 27, 28, 30, 31, 32, 33, 34, 36, 37, 38, 39, 40, 41, 44, 45, 46, 47, 48, 49, 50, 122, 123, 127, 131, 144, 152, 173, 175, 179, 180, 181, 182, 183, 185, 198, 200, 203, 206, 217, 226, 227, 241, 266, 267, 268, 271, 295, 309, 310, 334, 338, 379, 436, 448, 450, 455, 527

文献

Munakata, H., Nakada, T., Nakahigashi, K., Nozaki, H., Tomita, M. 2016 Phylogenetic position and molecular chronology of a colonial green flagellate, Stephanosphaera pluvialis (Volvocales, Chlorophyceae), among unicellular algae. J. Eucaryot. Microbiol., 63, 340-348.
キーワード: Balticola; coloniality; divergence time; evolution; molecular phylogeny
Strain(s): 123, 144, 421, 428, 432, 439, 446, 567, 569, 571, 656, 861, 887, 968, 1048, 2293, 2564, 4124, 4125
PubMed: 26595722
DOI: 10.1111/jeu.12283

Nakada, T., Nozaki, H., Pröschold, T. 2008 Molecular phylogeny, ultrastructure, and taxonomic revision of Chlorogonium (Chlorophyta): emendation of Chlorogonium and description of Gungnir gen. nov. and Rusalka gen.nov. J. Phycol., 44, 751-760.
キーワード: Chlorogonium; Gungnir gen.nov.; molecular phylogeny; morphology; Rusalka gen.nov.; taxonomy; ultrastructure; Volvocales
Strain(s): 123, 439, 692, 751, 755, 761, 1357, 1359, 1360, 1869, 4125
DOI: 10.1111/j.1529-8817.2008.00525.x

Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55.
キーワード: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection
Strain(s): 19, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 44, 45, 46, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 73, 74, 75, 76, 77, 78, 79, 80, 81, 87, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 119, 120, 122, 123, 124, 125, 127, 128, 129, 130, 131, 132, 133, 134, 135, 137, 138, 139, 144, 147, 148, 150, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 162, 163, 164, 165, 166, 167, 168, 169, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 185, 186, 187, 188, 189, 191, 192, 193, 194, 195, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 224, 226, 227, 228, 229, 230, 231, 232, 241, 242, 243, 244, 245, 246, 248, 257, 259, 261, 262, 263, 266, 267, 268, 271, 287, 288, 289, 290, 294, 295, 297, 298, 299, 300, 301, 302, 303, 305, 306, 308, 309, 310, 311, 312, 313, 329, 334, 336, 337, 338, 339, 340, 341, 342, 349, 351, 359, 360, 361, 362, 375, 378, 379, 380, 382, 384, 385, 390, 394, 396, 397, 398, 415, 416, 418, 419, 421, 422, 423, 424, 425, 426, 427, 428, 429, 430, 431, 432, 433, 434, 436, 437, 438, 439, 440, 446, 447, 448, 449, 450, 451, 452, 453, 454, 455, 456, 457, 458, 459, 460, 464, 468, 469, 474, 478, 479, 480, 481, 503, 504, 505, 506, 507, 509, 510, 512, 514, 515, 522, 523, 524, 527, 528, 529, 530, 531, 532, 536, 537, 538, 539, 540, 541, 542, 543, 544, 545, 546, 564, 565, 566, 567, 568, 569, 570, 571, 572, 573, 574, 575, 576, 577, 578, 579, 580, 581, 582, 583, 584, 585, 592, 593, 594, 595, 596, 597, 598, 604, 610, 628, 630, 631, 632, 633, 634, 635, 636, 637, 638, 639, 640, 641, 642, 643, 644, 645, 646, 647, 648, 649, 650, 651, 652, 653, 654, 655, 656, 657, 658, 659, 660, 661, 662, 663, 664, 665, 666, 667, 672, 685, 686, 687, 688, 689, 690, 691, 692, 693, 694, 717, 718, 719, 720, 721, 722, 723, 724, 725, 726, 727, 728, 729

Nozaki, H., Onishi, K., Morita, E. 2002 Differences in pyrenoid morphology are correlated with differences in the rbcL genes of members of the Chloromonas lineage (Volvocales, Chlorophyceae). J. Mol. Evol., 55, 414-430.
キーワード: ATP synthase β-subunit (atpB) gene; Chloromonas; gene evolution; large subunit of Rubisco (rbcL) gene; morphological evolution; P700 chlorophyll a-apoprotein A2 (psaB) gene; pyrenoid; Volvocales
Strain(s): 96, 123, 144, 213, 418, 421, 425, 428, 432, 439, 446, 447, 456, 474, 522, 564, 566, 567, 569, 571, 574, 582, 653, 732, 851, 858, 861, 875, 882, 883, 968
PubMed: 12355262
DOI: 10.1007/s00239-002-2338-9

Nozaki, H., Ohta, N., Morita, E., Watanabe, M. M. 1998 Toward a natural system of species in Chlorogonium (Volvocales, Chlorophyta): a combined analysis of morphological and rbcL gene sequence data. J. Phycol., 34, 1024-1037.
キーワード: Chlorogonium; Chlorogonium kasakii sp. nov.; Chlorophyta; molecular phylogeny; monophyletic species; morphology; natural systematics; rbcL gene; species taxonomy; Volvocales
Strain(s): 123, 439, 446, 692, 742, 743, 744, 745, 746, 747, 748, 749, 750, 751, 752, 753, 754, 755, 756, 757, 758, 759, 760, 761
DOI: 10.1046/j.1529-8817.1998.341024.x

Nozaki, H., Aizawa, K., Watanabe, M. M. 1996 Re-examination of two NIES strains labeled Chlorogonium metamorphum (Volvocales, Chlorophyta) from Japan. Nova Hedwigia Beiheft, 112, 483-490.
キーワード: Chlorogonium; Chlorophyta; morphology; neotype strain; species identification; Volvocales
Strain(s): 123, 446

Ichimura, T., Itoh, T. 1977 17. Preservation methods of microalgae (I) [17. Bisaisôrui no hozonhô (I)]. In Preservation methods of microorganisms [Biseibutsu Hozonhô], Ed. by Nei, T., University of Tokyo Press, Tokyo, p. 355-373 (in Japanese without English title).
Strain(s): 19, 21, 22, 23, 24, 27, 28, 30, 31, 32, 33, 34, 36, 37, 38, 39, 40, 41, 44, 45, 46, 47, 48, 49, 50, 122, 123, 127, 131, 144, 152, 173, 175, 179, 180, 181, 182, 183, 185, 198, 200, 203, 206, 217, 226, 227, 241, 266, 267, 268, 271, 295, 309, 310, 334, 338, 379, 436, 448, 450, 455, 527

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