株番号 NIES-68  
門名 Streptophyta  
綱名 Charophyceae 
学名 Closterium peracerosum-strigosum-littorale complex  
シノニム  
以前使われていた種名  
一般名 接合藻 ; ミカヅキモ ; ヒメミカヅキモ  
地名(採集年月) ネパール Damchan (1965-11-05)  
緯度/経度  
生息環境(分離源) 淡水 (土)  
分離後の履歴 ← 市村輝宜  
分離者(分離年月) 市村輝宜  
同定者 市村輝宜  
株の状態 継代培養; 無菌[2013 Nov]; 単藻; クローン  
培養条件
(前培養条件)
培地名:  C  
温度: 15 (20) ℃
光強度: 7-14 (10-17) µmol photons/m2/sec,  明暗周期: 10L:14D
継代培養周期: 3 M (21 D)  
遺伝子情報 18S rRNA ( AB363692 ) , 5.8S-ITS2-28S ( AB625555 ) , CpCAB1 ( AB363403 ) , CpHSP70 ( AB363402 ) , CpMADS1 mRNA ( AB091476 ) , CpPI mRNA ( AB012698 ) , ESTs(cDNA) ( AU294770-AU295959 ) , chloroplast phosphoribulokinas ( AB650527 )  
特性 生物指標 ; 雌雄異株: 交配型 (−) ; 交配群I B  
サイズ (min - max) 90 - 110 μm  
体制 単細胞性 
その他の株番号 その他の株番号:N-31-24  
注意事項 無菌  
動画  
文献
Sekimoto, H. 2017 Sexual reproduction and sex determination in green algae. J. Plant Res., 130, 423-431.
キーワード: Closterium; Chlamydomonas; Sexual reproduction; Sex determination
Strain(s): 6768 
PubMed: 28188480
DOI: 10.1007/s10265-017-0908-6

Tsuchikane, Y., Tsuchiya, M., Hindák, F., Nozaki, H., Sekimoto, H. 2012 Zygospore formation between homothallic and heterothallic strains of Closterium. Sex. Plant Reprod., 25, 1-9.
キーワード: Closterium; Conjugation; Homotallism; Heterothallism: Dexual reproduction; Zygospore
Strain(s): 5859606162636465676869702612666 
PubMed: 21858438
DOI: 10.1007/s00497-011-0174-z

Hori, S., Sekimoto, H., Abe, J. 2012 Properties of cell surface carbohydrates in sexual reproduction of the Closterium peracerosum–strigosum–littorale complex (Zygnematophyceae, Charophyta). Phycol. Res., 60, 254-260.
キーワード: cell-cell recognition; Closterium; ConcanavalinA; conjugation; lectin; Lycopersicon esculentum lectin; sexual reproduction
Strain(s): 6768 
DOI: 10.1111/j.1440-1835.2012.00656.x

Abe, J., Hori, S., Tsuchikane, Y., Kitao, N., Kato, M., Shiraiwa, H. 2011 Stable nuclear transformation of the Closterium peracerosum–strigosum–littorale complex. Plant Cell Physiol., 52, 1676-1685.
Strain(s): 6768 
PubMed: 21804095
DOI: 10.1093/pcp/pcr103

Tsuchikane, Y., Ito, M., Sekimoto, H. 2008 Reproductive isolation by sex pheromones in the Closterium peracerosum-strigosum-littorale complex (Zygnematales, Charophyceae). J. Phycol., 44, 1197-1203.
キーワード: Closterium; conjugation; pheromone; reproductive isolation; sexual reproduction
Strain(s): 53545859646567686970229261 
PubMed: 27041716
DOI: 10.1111/j.1529-8817.2008.00577.x

Abe, J., Hiwatashi, Y., Ito, M., Hasebe, M., Sekimoto, H. 2008 Expression of exogenous genes under the control of endogenous HSP70 and CAB promoters in the Closterium peracerosum–strigosum–littorale complex. Plant Cell Physiol., 49, 625-632.
Strain(s): 68 
PubMed: 18308758
DOI: 10.1093/pcp/pcn039

Imaizumi, M., Sekimoto, H., Noguchi, T. 2007 Induction of sex pheromone secretion and observations of the secretory pathway in the Closterium peracerosum-strigosum-littorale complex. Plant Sci., 173, 206-212.
キーワード: Closterium peracerosum-strigosum-littorale; Immunoelectron microscopy; Protoplast release-inducing protein; Sex pheromone; Sexual reproduction; Zygote formation
Strain(s): 6768 
DOI: 10.1016/j.plantsci.2007.05.002

Akatsuka, S., Tsuchikane, Y., Fukumoto, R., Fujii, T., Sekimoto, H. 2006 Physiological characterization of the sex pheromone protoplast-release-inducing protein from the Closterium peracerosum-strigosum-littorale complex (Charophyta). Phycol. Res., 54, 116-121.
キーワード: Closterium; conjugation; mucilage; protoplast release; sex pheromone; sexual reproduction
Strain(s): 6768 
DOI: 10.1111/j.1440-1835.2006.00418.x

Hamada, S., Sekimoto, H., Tanabe, Y., Tsuchikane, Y., Ito, M. 2006 Isolation of myosin XI genes from the Closterium peracerosum-strigosum-littorale complex and analysis of their expression during sexual reproduction. J. Plant Res., 119, 105-113.
キーワード: Closterium; Cytokinesis; Myosin XI; Real-time PCR; Sexual cell division; Sexual reproduction
Strain(s): 656768 
PubMed: 16456621
DOI: 10.1007/s10265-005-0249-8

Tanabe, Y., Hasebe, M., Sekimoto, H., Nishiyama, T., Kitani, M., Henschel, K., Münster, T., Theißen, G., Nozaki, H., Ito, M. 2005 Characterizaion of MADS-box genes in charophycean green alga and its implication for the evolution of MADS-box genes. Proc. Nati. Acad. Sci. U. S. A., 102, 2436-2441.
キーワード: charophyve; land plants; Chara; Coleochaete; Closterium
Strain(s): 6768 
PubMed: 15699346
DOI: 10.1073/pnas.0409860102

Tsuchikane, Y., Fujii, T., Ito, M., Sekimoto, H. 2005 A sex pheromone, protoplast release-inducing protein (PR-IP) inducer, induces sexual cell division and production of PR-IP in Closterium. Plant Cell Physiol., 46, 1472-1476.
キーワード: Closterium; conjugation; pheromone; sexual cell division; sexual reproduction
Strain(s): 6768 
PubMed: 15970600
DOI: 10.1093/pcp/pci158

Sekimoto, H. 2004 Molecular characterization of a novel sex pheromone, PR-IP inducer, in Closterium peracerosum-strigosum-littorale complex. Jpn. J. Phycol., 52, Suppl., 117-122.
キーワード: Closterium; conjugation; gamete mating; sex pheromone, sexual reproduction
Strain(s): 6768 

Tsuchikane, Y., Fukumoto, R., Akatsuka, S. Fujii, T., Sekimoto, H. 2003 Sex pheromones that induce sexual cell division in the Closterium peracerosum-strigosum-littorale complex (Charophyta). J. Phycol., 39, 303-309.
キーワード: Closterium; conjugation; gametangial cell; pheromone; sexual cell division; sexual reproduction
Strain(s): 6768 
DOI: 10.1046/j.1529-8817.2003.02062.x

Sekimoto, H., Tanabe, Y., Takizawa, M., Ito, N., Fukumoto, R., Ito, M. 2003 Expressed sequence tags from Closterium peracerosum-strigosum-littorale complex, a unicellular charophycean alga, in the sexual reproduction process. DNA Res., 10, 147-153.
キーワード: Closterium; conjugation; EST; sexual reproduction
Strain(s): 6768 
PubMed: 14621293
DOI: 10.1093/dnares/10.4.147

Akatsuka, S., Sekimoto, H., Iwai, H., Fukumoto, R., Fujii, T. 2003 Mucilage secretion regulated by sex pheromones in Closterium peracerosum-strigosum-littorale complex. Plant Cell Physiol., 44, 1081-1087.
キーワード: Closterium; mucilage; sex pheromone; sexual reproduction
Strain(s): 6768 
DOI: 10.1093/pcp/pcg131

Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55.
キーワード: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection
Strain(s): 19212223242526272829303132333435363738394041444546505152535455565758596061626364656667686970737475767778798081878990919293949596979899100101102103104105106107108109110111112119120122123124125127128129130131132133134135137138139144147148150151152153154155156157158159160162163164165166167168169170171172173174175176177178179180181182183185186187188189191192193194195197198199200201202203204205206207208209210211212213214215216217224226227228229230231232241242243244245246248257259261262263266267268271287288289290294295297298299300301302303305306308309310311312313329334336337338339340341342349351359360361362375378379380382384385390394396397398415416418419421422423424425426427428429430431432433434436437438439440446447448449450451452453454455456457458459460464468469474478479480481503504505506507509510512514515522523524527528529530531532536537538539540541542543544545546564565566567568569570571572573574575576577578579580581582583584585592593594595596597598604610628630631632633634635636637638639640641642643644645646647648649650651652653654655656657658659660661662663664665666667672685686687688689690691692693694717718719720721722723724725726727728729 

Sekimoto, H. 2002 Production and secretion of a biologically active Closterium sex pheromone by Saccharomyces cerevisiae. Plant Physiol. Biochem., 40, 789-794.
キーワード: Closterium; conjugation; pheromone; PR-IP; sexual reproduction
Strain(s): 6768 
DOI: 10.1016/S0981-9428(02)01432-8

Sekimoto, H., Fukumoto, R. 1999 Sex pheromones in Closterium. Chem. Regul. Plant, 34, 305-315.
Strain(s): 64656768 

Sekimoto, H., Fukumoto, R., Dohmae, N., Takio, K. Fujii, T., Kamiya, Y. 1998 Molecular cloning of a novel sex pheromone responsible for the release of a different sex pheromone in Closterium peracerosum-strigosum-littorale complex. Plant Cell Physiol., 39, 1169-1175.
キーワード: Clostreium; conjugation; gamete; mating; sex pheromone; sexual reproduction
Strain(s): 6768 
DOI: 10.1093/oxfordjournals.pcp.a029317

Endo, B., Fujii, T., Kamiya, Y., Sekimoto, H. 1997 Analysis of genomic sequences encoding a sex pheromone from the Closterium peracerosum-strigosum-littorale complex. J. Plant Res., 110, 463-467.
キーワード: cis-element; Closterium; genomic DNA; protoplast-release- inducing protein (PR-IP); sex pheromone; transcription
Strain(s): 6768 

Nojiri, T., Fujii, T., Sekimoto, H. 1995 Purification and characterization of a novel sex pheromone that induces the release of another sex pheromone during sexual reproduction of the heterothallic Closterium peracerosum-strigosum-littorale complex. Plant Cell Physiol., 36, 79-84.
キーワード: Closterium; conjugation (Closterium); protoplast-release-inducing protein; pheromone
Strain(s): 6768 
DOI: 10.1093/oxfordjournals.pcp.a078747

Sekimoto, H., Sone, Y., Fujii, T. 1995 Biochemical, physiological, and molecular analysis of sexual isolation in the species complex Closterium peracerosum-strigosum-littorale (Chlorophyta). J. Phycol., 31, 611-615.
キーワード: Chlorophyta; Closterium peracerosum-strigosum-littorale complex; conjugation; pheromone; plotoplast-release-inducing protein (PR-IP); sexual isolation
Strain(s): 64656768 
DOI: 10.1111/j.1529-8817.1995.tb02557.x

Sekimoto, H., Sone, Y., Fujii, T. 1994 Regulation of expression of the genes for a sex pheromone by an inducer of the sex pheromone in the Closterium peracerosum-strigosum-littorale complex. Planta, 193, 137-144.
キーワード: Clostreium complex; inducer (of protoplast-release-inducing protein); pheromone; protoplast-release-inducing protein (PR-IP); sexual reproduction
Strain(s): 6768 
PubMed: 7764622
DOI: 10.1007/BF00191617

Sekimoto, H., Fujii, T. 1993 Cell-cell interaction involved in the sexual reproduction of Closterium [Mikadukimo no yûsei seisyoku ni kakawaru saibôkan jôhô kôkan]. Iden, 47 (1), 43-48 (in Japanese without English title).
Strain(s): 64656768 

Sekimoto, H., Satoh, S., Fujii, T. 1993 Analysis of binding of biotinylated protoplast-release-inducing protein that induces release of gametic protoplasts in the Closterium peracerosum-strigosum-littorale complex. Planta, 189, 468-474.
キーワード: Clostreium; conjugation (Closterium); protoplast-release; inducing protein; pheromone; receptor
Strain(s): 6768 
PubMed: 24178507
DOI: 10.1007/BF00194447

Sekimoto, H., Fujii, T. 1992 Analysis of gametic protoplast release in the Closterium peracerosum-strigosum-littorale complex (Chlorophyta). J. Phycol., 28, 615-619.
キーワード: biologically active glycoprotein; Closterium peracerosum-strigosum-littorale complex; conjugation; gametic protoplast release
Strain(s): 6768 
DOI: 10.1111/j.0022-3646.1992.00615.x

Sekimoto, H., Satoh, S., Fujii, T. 1992 Biochemical and physiological properties of a gametic protoplast-release-inducing protein in Closterium. Korean J. Phycol., 7, 121-129.
キーワード: Clostreium; conjugation; glycoprotein; protoplast release in conjugation
Strain(s): 6768 

Sekimoto, H., Fujii, T. 1991 Sexual reproduction of microalgae : its physiological and biochemical study. Cell Science, 7, 562-571 (in Japanese with English title).
Strain(s): 6768 

Sekimoto, H., Satoh, S., Fujii, T. 1990 Biochemical and physiological properties of a protein inducing protoplast release during conjugation in the Closterium peracerosum-strigosum-littorale complex. Planta, 182, 348-354.
キーワード: Clostreium; conjugation (Closterium); glycoprotein; protoplast release in conjugation
Strain(s): 6768 
DOI: 10.1007/BF02411384

Watanabe, M. M. 1977 Biosystematics in Closterium of sexual unicellular green algae and Calothrix and Spirulina of asexual filamentous blue-green algae, with special reference to the analyses of natural populations. Thesis D. Sci., Hokkaido University, 114 pp., 28 tables, 54 figs.
Strain(s): 3945465152535455565758596061626364656667686970261262266268 

Ichimura, T. 1973 The life cycle and its control in some species of Closterium, with special reference to the biological species problems. Thesis D. Sci., University of Tokyo, 69 pp., 11 tables, 40 figs.
Strain(s): 666768124125127174175176177179180181182183185198202262337338450451 

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