Strain number NIES-3874  
Phylum Chlorophyta  
Class Chlorophyceae  
Scientific name Volvox capensis M.F.Rich & M.A.Pocock  
Synonym  
Former name  
Common name Green alga ; Volvocine alga  
Locality (Date of collection) A marsh connected to Ninepipe Reservoir, Montana, USA (2014-07-28)  
Latitude / Longitude 47.44041138 /  
Habitat (Isolation source) Freshwater (Soil)  
History < Nozaki, Hisayoshi  
Isolator (Date of isolation) Nozaki, Hisayoshi (2014-09-03)  
Identified by Nozaki, Hisayoshi  
State of strain Subculture; Mixed; Unialgal; Clonal; Axenic[2022 Apr]  
Culture condition
(Preculture condition)
Medium:  VTAC  
Temperature:  15 C
Light intensity:  0 µmol photons/m2/sec,
Duration:  7 D  
Gene information rbcL ( LC033870 ) , psbC ( LC033872 ) , ITS1-5.8S-ITS-26S ( LC034074 ) , MID ( LC699263 )  
Cell size (min - max)  
Organization Colonial; Flagellate 
Characteristics Mixotrophic ; Phototaxis ; Homothallic ; Monoecious ; Oogamy ; Resting zygote forming ; When grow in light-dark condition, sexual spheroides develope to form zygotes and do not reproduce asexually to maintain the culture.  
Other strain no. Other strain no. : M1-2  
Remarks Axenic; Fragile species to transportation stresses; Read and agree "How to order 4.1" 
Movie  
Reference
Yamagishi, S., Yamamoto, K., Takahashi, K., Kawai-Toyooka, H., Suzuki, S., Matsuzaki, R., Yamaguchi, H., Kawachi, M., Higashiyama, T., Nozaki, H. 2023 Evolutionary analysis of MID homologs during the transition from homothallic species to heterothallic species in Volvox sect. Volvox (Chlorophyceae). Phycol. Res., 72, 46-55.
Keywords: male gamete; mating system; minus dominance gene (MID); molecular evolution; sex; sperm packet; volvocine green algae
Strain(s): 28945646054154454556957257458265265365473285185285787289189213621363170817101859245725692735273627403382338337803782378638743984398639884021402543364432453545364537 
DOI: 10.1111/pre.12538

Nozaki, H., Mahakham, W., Heman, W., Matsuzaki, R., Kawachi, M. 2020 A new preferentially outcrossing monoicous species of Volvox sect. Volvox (Chlorophyta) from Thailand. PLoS One, 15, e0235622 (article ID).
Strain(s): 273627403382338338744021433644324433443444354436 
PubMed: 32614898
DOI: 10.1371/journal.pone.0235622

Nozaki, H., Ueki, N., Misumi, O., Yamamoto, K., Yamashita, S., Herron, M. D., Rosenzweig, F. 2015 Morphology and reproduction of Volvox capensis (Volvocales, Chlorophyceae) from Montana, USA. Phycologia, 54, 316-320.
Keywords: Monoecious species; Morphology; Pplyspermy; Reproduction; Volvox capensis; Volvox sect. Volvox
Strain(s): 3874 
DOI: 10.2216/15-14.1

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