A big image opens when the thumbnail is clicked.(nies-0257.jpg)
Strain number NIES-257  
Phylum Chlorophyta  
Class Chlorophyceae  
Scientific name Hafniomonas laevis Nakada, Suda & Nozaki  
Synonym  
Former name Hafniomonas montana (Geitler) Ettl et Moestrup  
Common name Green alga  
Locality (Date of collection) inside NIES, Onogawa, Tsukuba, Ibaraki, Japan (1983-10-30)  
Latitude / Longitude 36.048471 / 140.117609 
Habitat (Isolation source) Freshwater (Pool water)  
History < Suda, Shoichiro  
Isolator (Date of isolation) Suda, Shoichiro (1983-10-30)  
Identified by Inouye, Isao; Nakada, et al. (Reidentify)  
State of strain Subculture; Unialgal; Clonal; Axenic[2017 Dec]  
Culture condition
(Preculture condition)
Medium:  C  
Temperature:  20 (20) C
Light intensity:  6-8 (10-17) µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  1 M (14 D)  
Gene information Plastid DNA ( KT625415 ) , 18S rRNA ( AB101517 ) , atpB ( AB101504 ) , rbcL ( AB101510 ) , psaB ( AB101515 )  
Cell size (min - max) 15 - 17 μm  
Organization Unicellular; Flagellate 
Characteristics Authentic strain (Nakada et al. 2007) ; Genome decoded strain (Lemieux et al. 2015)  
Other strain no. Other strain no. : OUT-5  
Remarks Axenic 
Movie  
Reference
Sausen, N., Malavasi, V., Melkonian, M. 2018 Molecular phylogeny, systematics and revision of the type species of Lobomonas, L. francei,(Volvocales, Chlorophyta) and closely related taxa. J. Phycol., 54, 198-214.
Keywords: crystalline glycoprotein lattice; CWClade; ITS2; L. panduriformis; rbcL; Reinhardtinia; scanning electron microscopy; SSU nrRNA
Strain(s): 25741845647454156657766673587988088288313621708185825673923 
PubMed: 29278416
DOI: 10.1111/jpy.12615

Lemieux, C., Vincent, A. T., Labarre, A., Otis, C., Turmel M. 2015 Chloroplast phylogenomic analysis of chlorophyte green algae identifies a novel lineage sister to the Sphaeropleales (Chlorophyceae). BMC Evol. Biol., 15, 264 (article ID).
Keywords: Chlorophyceae; Chlamydomonadales; Golenkiniaceae; Treubarinia, Jenufa genus; Plastid genome; Flagellar apparatus
Strain(s): 257425 
PubMed: 26620802
DOI: 10.1186/s12862-015-0544-5

Nakada, T., Suda, S., Nozaki, H. 2007 A taxonomic study of Hafniomonas (Chlorophyceae) based on a comparative examination of cultured material. J. Phycol., 43, 397-411.
Keywords: Chlorophyceae; Hafniomonas; morphology; new species; taxonomy
Strain(s): 257656171417151716171717181720 
DOI: 10.1111/j.1529-8817.2007.00328.x

Nakazawa, A. & Nozaki, H. 2004 Phylogenetic analysis of the Tetrasporalean genus Asterococcus (Chlorophyceae) based on 18S ribosomal RNA gene sequences. J. Jpn. Bot., 79, 255-261.
Keywords: 18S rRNA gene; Asterococcus; Palmellopsidaceae; phylogeny; Tetrasporales
Strain(s): 257656 

Nozaki, H., Misumi, O., Kuroiwa, T. 2003 Phylogeny of the quadriflagellate Volvocales (Chlorophyceae) based on chloroplast multigene sequences. Mol. Phylogenet. Evol., 29, 58-66.
Strain(s): 257656 
PubMed: 12967607
DOI: 10.1016/S1055-7903(03)00089-7

Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55.
Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection
Strain(s): 192122232425262728293031323334353637383940414445465051525354555658596061626364656667686970737475767778798081878990919293949596979899100101102103104105106107108109110111112119120122123125127128129130131132133134135137138139144147151152153154155156157158159160162163164165166167168170171172173174175176177178179180181182183185186187188189191192193194197198199200201202203204205206207208209210211212213214215216217224226227228229230231232241242243244245246248257259261262263266267268287288289290294295297298299300301302303305306308309310312313329334337338339340341342349351359360361362375378379380382384385390394396397398415416418419421422423424425426427428429430431432433434436437438439440446447448449450451452453454455456457458459460464468469474478479480481503504505506507509510512514515522523524527528529530531532536537538539540541542543544545546564565566567568569570571572573574575576577578579580581582583584585592593594595596597598604610628630631632633634635636637638639640641642643644645646647648649650651652653654655656657658659660661662663664665666667672685686687688689690691692693694717718719720721722723724725726727728729 

Suda, S. & Watanabe, M. M. 1995 Notes on microalgae in Japan (2). Hafniomonas montana (Geitler) Ettl et Moestrup (Chlorophyceae, Dunaliellales). Jpn. J. Phycol. (Sôrui), 43, 40-41 (in Japanese with English title).
Strain(s): 257 

Watanabe, M. M., Kasai, F., Hiwatari, T., Suda, S., Nei, T. 1984 Cryopreservation of microalgae in liquid nitrogen - Survival of algal cultures after freezing. Jpn. J. Freez. Dry., 30, 23-26 (in Japanese with English title).
Strain(s): 1819212426293139404144454650737475767780818788899091939598991001011201221311351371441512042072132572633794152219223522392579 

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