Strain number NIES-656  
Phylum Chlorophyta  
Class Chlorophyceae  
Scientific name Hafniomonas montana (Geitler) Ettl & Moestrup  
Synonym  
Former name  
Common name Green alga  
Locality (Date of collection) Tsukuba, Ibaraki, Japan (1986-04-30)  
Latitude / Longitude 36.105319 / 140.081738 
Habitat (Isolation source) Freshwater (Pond sediment)  
History < Suda, Shoichiro  
Isolator (Date of isolation) Suda, Shoichiro (1986-05-02)  
Identified by Suda, Shoichiro  
State of strain Subculture; Unialgal; Clonal; Axenic[2017 Dec]  
Culture condition
(Preculture condition)
Medium:  C  
Temperature:  20 C
Light intensity:  3 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  1 M  
Gene information 18S rRNA ( AB101518 ) , atpB ( AB101505 ) , psaB ( AB101516 ) , rbcL ( AB101512 ) , psbO ( AB293974 )  
Cell size (min - max)  
Organization Unicellular; Flagellate 
Characteristics Authentic strain, Epitype (Nakada et al. 2007)  
Other strain no. Other strain no. : 430M3-3  
Remarks Axenic 
Movie  
Reference
Munakata, H., Nakada, T., Nakahigashi, K., Nozaki, H., Tomita, M. 2016 Phylogenetic position and molecular chronology of a colonial green flagellate, Stephanosphaera pluvialis (Volvocales, Chlorophyceae), among unicellular algae. J. Eucaryot. Microbiol., 63, 340-348.
Keywords: Balticola; coloniality; divergence time; evolution; molecular phylogeny
Strain(s): 12314442142843243944656756957165686188796810482293256441244125 
PubMed: 26595722
DOI: 10.1111/jeu.12283

Nakada, T., Suda, S., Nozaki, H. 2007 A taxonomic study of Hafniomonas (Chlorophyceae) based on a comparative examination of cultured material. J. Phycol., 43, 397-411.
Keywords: Chlorophyceae; Hafniomonas; morphology; new species; taxonomy
Strain(s): 257656171417151716171717181720 
DOI: 10.1111/j.1529-8817.2007.00328.x

Takahashi, F., Okabe, Y., Nakada, T., Sekimoto, H., Ito, M., Kataoka, H. & Nozaki, H. 2007 Origins of the secondary plastids of Euglenophyta and Chlorarachniophyta as revealed by an analysis of the plastid-targeting, nuclear-encoded gene psbO. J. Phycol., 43, 1302-1309.
Keywords: alanine-X-alanine motif; Chlorarachniophyta; Euglenophyta; phylogeny; plastids; psbO; secondary endosymbiosis; transit peptide; thylakoid-targeting domain
Strain(s): 67203254381483533624656 
DOI: 10.1111/j.1529-8817.2007.00411.x

Nakazawa, A. & Nozaki, H. 2004 Phylogenetic analysis of the Tetrasporalean genus Asterococcus (Chlorophyceae) based on 18S ribosomal RNA gene sequences. J. Jpn. Bot., 79, 255-261.
Keywords: 18S rRNA gene; Asterococcus; Palmellopsidaceae; phylogeny; Tetrasporales
Strain(s): 257656 

Nozaki, H., Misumi, O., Kuroiwa, T. 2003 Phylogeny of the quadriflagellate Volvocales (Chlorophyceae) based on chloroplast multigene sequences. Mol. Phylogenet. Evol., 29, 58-66.
Strain(s): 257656 
PubMed: 12967607
DOI: 10.1016/S1055-7903(03)00089-7

Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55.
Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection
Strain(s): 192122232425262728293031323334353637383940414445465051525354555658596061626364656667686970737475767778798081878990919293949596979899100101102103104105106107108109110111112119120122123125127128129130131132133134135137138139144147151152153154155156157158159160162163164165166167168170171172173174175176177178179180181182183185186187188189191192193194197198199200201202203204205206207208209210211212213214215216217224226227228229230231232241242243244245246248257259261262263266267268287288289290294295297298299300301302303305306308309310312313329334337338339340341342349351359360361362375378379380382384385390394396397398415416418419421422423424425426427428429430431432433434436437438439440446447448449450451452453454455456457458459460464468469474478479480481503504505506507509510512514515522523524527528529530531532536537538539540541542543544545546564565566567568569570571572573574575576577578579580581582583584585592593594595596597598604610628630631632633634635636637638639640641642643644645646647648649650651652653654655656657658659660661662663664665666667672685686687688689690691692693694717718719720721722723724725726727728729 

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