Strain Data

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Strain number		NIES-39
Phylum		Cyanophyta
Class		Cyanophyceae
Scientific name		Arthrospira platensis Gomont
Synonym		Oscillatoria platensis (Gomont) Bourrelly 1970
Former name		Spirulina platensis (Gomont) Geitler
Common name		Blue-green alga ; Cyanobacteria
Locality (Date of collection)		Lake Chad,
Latitude / Longitude
Habitat (Isolation source)		Salt water
History		< IAM (1983);
Isolator (Date of isolation)
Identified by
State of strain		Subculture; Unialgal; Clonal; Axenic[2018 Feb]
Culture condition (Preculture condition)		Medium: SOT Temperature: 20 (25) C Light intensity: 6-8 (12) µmol photons/m²/sec, L/D cycle: 10L:14D Duration: 4 M (14 D)
Gene information		Whole-genome ( AP026945 AP011615 ) , 16S rRNA ( AB074508 DQ393279 ) , cpcB, cpcA ( DQ393287 ) , crtB ( AB001284 ) , cyaA ( D49530 ) , cyaG ( D49531 ) , cyaC ( D49692 ) , groEL ( AB039632 ) , gyrB ( AB074765 ) , petH ( AB113346 ) , MS ( AB548759 ) , mth ( AB282753 ) , recA ( U33924 ) , rpoC1 ( AB074788 ) , rpoD1 ( AB074815 )
Cell size (min - max)		6 - 7 μm
Organization		Filamentous
Characteristics		Autotrophic ; Genome decoded strain (Fujisawa et al. 2010) ; Hydrogen evolution ; Contains good quality of proteins
Other strain no.		Other collection strain no. : IAM M-135 (=M-222); UTEX 3086
Remarks		Axenic
Movie

Reference
Shiraishi, H. & Nishida, H. 2023 Complete genome sequence of the edible filamentous cyanobacterium Arthrospira platensis NIES-39, based on long-read sequencing. Microbiol. Resour. Ann., 12, e0113922 (article ID). Strain(s): 39 PubMed: 36537808 DOI: 10.1128/mra.01139-22 Sugiyama, K., Takahashi, K., Nakazawa, K., Yamada, M., Kato, S., Shinomura, T., Nagashima, Y., Suzuki, H., Ara, T., Harada, J., Takaichi, S. 2020 Oxygenic phototrophs need ζ-carotene isomerase (Z-ISO) for carotene synthesis: Functional analysis in Arthrospira and Euglena. Plant Cell Physiol., 61, 276–282. Keywords: Arthrospira; CarotenoidEuglena; Oxygenic phototrophs; Z-ISO; ζ-carotene isomerase Strain(s): 39 PubMed: 31593237 DOI: 10.1093/pcp/pcz192 Tanaka, K., Kishi, M., Assaye, H., Toda, T. 2020 Low temperatures in dark period affect biomass productivity of a cyanobacterium Arthrospira platensis. Algal Res., 52, 102132 (article ID). Keywords: Microalgae; Respiration; Temperature; Arthrospira platensis; Biomass productivity; Night biomass loss Strain(s): 39 DOI: 10.1016/j.algal.2020.102132 Komatsu, K., Onouchi, H., Imai, A., Kawasaki, N., Hashim, E. F., Mohd Rajuddin, M. K. 2019 Effects of dissolved organic matter in soil extracts on the growth of microalgae. J. Jpn. Soc. Wat. Environ., 42, 239-246 (in Japanese with English summary). Keywords: Soil extracts; Microalgae; Microplate technique; Excitation emission matrix; Molecular size distribution Strain(s): 39, 2168, 2256, 2257, 2258 DOI: 10.2965/jswe.42.239 Tanioka, Y. 2019 Food chemistry and physiological function of pseudovitamin B12. Vitamins (Japan), 93, 47-51 (in Japanese with English summary). Keywords: pseudovitamin B12; corrinoid compounds; cyanobacteria; meheionine synthase; shellfish Strain(s): 39 DOI: 10.20632/vso.93.2_47 Tadama, S. & Shiraishi, H. 2017 Growth of the edible microalga Arthrospira platensis in relation to boron supply. International Journal of GEOMATE, 12, 90-95. Keywords: Cyanobacteria; Spirulina; Arthrospira platensis; Boron, H-resorcinol Strain(s): 39 DOI: 10.21660/2017.30.2580 Sugiyama, K., Ebisawa, M., Yamada, M., Nagashima, Y., Suzuki, H., Maoka, T., Takaichi, S. 2017 Functional lycopene cyclase (CruA) in cyanobacterium, Arthrospira platensis NIES-39, and its role in carotenoid synthesis. Plant Cell Physiol., 58, 831-838. Keywords: Arthrospira; Carotenoid; Lycopene cyclase; crtR; cruA; β-Carotene hydroxylase Strain(s): 39 PubMed: 28371918 DOI: 10.1093/pcp/pcx015 Toyoshima, M., Aikawa, S., Yamagishi, T., Kondo, A., Kawai, H. 2015 A pilot-scale floating closed culture system for the multicellular cyanobacterium Arthrospira platensis NIES-39. J. Appl. Phycol., 27, 2191-2202. Keywords: Arthrospira (Spirulina) platensis NIES-39; Biomass productivity; Floating closed culture system; Modified seawater medium; Outdoor sulture Strain(s): 39 DOI: 10.1007/s10811-014-0484-2 Yoshikawa, K., Aikawa, S., Kojima, Y., Toya, Y., Furusawa, C., Kondo, A., Shimizu, H. 2015 Construction of a genome-scale metabolic model of Arthrospira platensis NIES-39 and metabolic design for cyanobacterial bioproduction. PLoS One, 10, e0144430 (article ID). Strain(s): 39 PubMed: 26640947 DOI: 10.1371/journal.pone.0144430 Yoshikawa, T., Ikeda, Y., Sakata, T., Maeda, H. 2011 Cloning and analysis of the ggpS gene from cyanobacteria Arthrospira spp. involved in the synthesis of an osmolyte glucosylglycerol. Biocontrol Science, 16, 55-61. Keywords: Arthrospira; ggpS; Glucosylglycerol; Osmolyte; Synechococcus Strain(s): 39 PubMed: 21719990 DOI: 10.4265/bio.16.55 Tanioka, Y., Miyamoto, E., Yabuta, Y., Ohnishi, K., Fujita, T., Yamaji, R., Misono, H., Shigeoka, S., Nakano, Y., Inui, H., Watanabe, F. 2010 Methyladeninylcobamide functions as the cofactor of methionine synthase in a cyanobacterium, Spirulina platensis NIES-39. FEBS Lett., 584, 3223-3226. Keywords: adeninylcobamide; cobalamin; cyanobacteria; methionine synthase; pseudovitamine B12; Spirulina platensis Strain(s): 39 PubMed: 20558164 DOI: 10.1016/j.febslet.2010.06.013 Yukino, T., Hayashi, M., Inoue, Y., Imamura, J., Nagano, N., Murata, H. 2005 Preparation of docosahexaenoic acid fortified Spirulina platensis and its lipid and fatty acid compositions. Nippon Suisan Gakkaishi, 71, 74-79 (in Japanese with English summary). Keywords: docosahexaenoic acid; DHA; Spirulina; foods with function claims Strain(s): 39 DOI: 10.2331/suisan.71.74 Toyomizu, M., Suzuki, K., Kawata, Y., Kojima, H., Akiba, Y. 2001 Effective transformation of the cyanobacterium Spirulina platensis using electroporation. J. Appl. Phycol., 13, 209-214. Keywords: Arthrospira platensis; cyanobacterium; blue-green alga; electroporation; feed enzyme; poultry; Spirulina; transformation Strain(s): 39 DOI: 10.1023/A:1011182613761 Watanabe, F., Miyamoto, E., Nakano, Y. 2001 Inactive corrinoid-compound significantly decreases in Spirulina platensis grown in a cobalt-deficient medium. J. Agric. Food Chem., 49, 5685-5688. Keywords: Vitamin B12; pseudovitamin B12; algal health food; spirulina tablet; intrinsic facto Strain(s): 39 PubMed: 11714378 DOI: 10.1021/jf010733i Suzuki, S., Tazawa, R., Miya, A., Adachi, T., Kanki, R., Toyobe, M., Oguchi, M. 1994 Gas revitalization by microalgae. I. Studies on species and culture condition. CELSS J., 7, 23-28 (in Japanese with English summary). Keywords: microalgae; Spirulina; SOT medium; culture condition; CO²/O² gas exchange system Strain(s): 39, 45 Watanabe, M. M., Kasai, F., Hiwatari, T., Suda, S., Nei, T. 1984 Cryopreservation of microalgae in liquid nitrogen - Survival of algal cultures after freezing. Jpn. J. Freez. Dry., 30, 23-26 (in Japanese with English title). Strain(s): 18, 29, 39, 45, 74, 75, 77, 95, 100, 120, 131, 135, 379 Watanabe, M. M. 1977 Biosystematics in Closterium of sexual unicellular green algae and Calothrix and Spirulina of asexual filamentous blue-green algae, with special reference to the analyses of natural populations. Thesis D. Sci., Hokkaido University, 114 pp., 28 tables, 54 figs. Strain(s): 39, 45, 51, 56, 66, 68 Watanabe, M. M. & Ichimura, T. 1977 Fresh- and salt-water forms of Spirulina platensis in axenic cultures. Bull. Jpn. Soc. Phycol., 25, *Suppl. (Mem. Iss. Yamada),* 371-377. Strain(s): 39, 45

Reference

Shiraishi, H. & Nishida, H. 2023 Complete genome sequence of the edible filamentous cyanobacterium Arthrospira platensis NIES-39, based on long-read sequencing. Microbiol. Resour. Ann., 12, e0113922 (article ID).
Strain(s): 39
PubMed: 36537808
DOI: 10.1128/mra.01139-22

Sugiyama, K., Takahashi, K., Nakazawa, K., Yamada, M., Kato, S., Shinomura, T., Nagashima, Y., Suzuki, H., Ara, T., Harada, J., Takaichi, S. 2020 Oxygenic phototrophs need ζ-carotene isomerase (Z-ISO) for carotene synthesis: Functional analysis in Arthrospira and Euglena. Plant Cell Physiol., 61, 276–282.
Keywords: Arthrospira; CarotenoidEuglena; Oxygenic phototrophs; Z-ISO; ζ-carotene isomerase
Strain(s): 39
PubMed: 31593237
DOI: 10.1093/pcp/pcz192

Tanaka, K., Kishi, M., Assaye, H., Toda, T. 2020 Low temperatures in dark period affect biomass productivity of a cyanobacterium Arthrospira platensis. Algal Res., 52, 102132 (article ID).
Keywords: Microalgae; Respiration; Temperature; Arthrospira platensis; Biomass productivity; Night biomass loss
Strain(s): 39
DOI: 10.1016/j.algal.2020.102132

Komatsu, K., Onouchi, H., Imai, A., Kawasaki, N., Hashim, E. F., Mohd Rajuddin, M. K. 2019 Effects of dissolved organic matter in soil extracts on the growth of microalgae. J. Jpn. Soc. Wat. Environ., 42, 239-246 (in Japanese with English summary).
Keywords: Soil extracts; Microalgae; Microplate technique; Excitation emission matrix; Molecular size distribution
Strain(s): 39, 2168, 2256, 2257, 2258
DOI: 10.2965/jswe.42.239

Tanioka, Y. 2019 Food chemistry and physiological function of pseudovitamin B12. Vitamins (Japan), 93, 47-51 (in Japanese with English summary).
Keywords: pseudovitamin B12; corrinoid compounds; cyanobacteria; meheionine synthase; shellfish
Strain(s): 39
DOI: 10.20632/vso.93.2_47

Tadama, S. & Shiraishi, H. 2017 Growth of the edible microalga Arthrospira platensis in relation to boron supply. International Journal of GEOMATE, 12, 90-95.
Keywords: Cyanobacteria; Spirulina; Arthrospira platensis; Boron, H-resorcinol
Strain(s): 39
DOI: 10.21660/2017.30.2580

Sugiyama, K., Ebisawa, M., Yamada, M., Nagashima, Y., Suzuki, H., Maoka, T., Takaichi, S. 2017 Functional lycopene cyclase (CruA) in cyanobacterium, Arthrospira platensis NIES-39, and its role in carotenoid synthesis. Plant Cell Physiol., 58, 831-838.
Keywords: Arthrospira; Carotenoid; Lycopene cyclase; crtR; cruA; β-Carotene hydroxylase
Strain(s): 39
PubMed: 28371918
DOI: 10.1093/pcp/pcx015

Toyoshima, M., Aikawa, S., Yamagishi, T., Kondo, A., Kawai, H. 2015 A pilot-scale floating closed culture system for the multicellular cyanobacterium Arthrospira platensis NIES-39. J. Appl. Phycol., 27, 2191-2202.
Keywords: Arthrospira (Spirulina) platensis NIES-39; Biomass productivity; Floating closed culture system; Modified seawater medium; Outdoor sulture
Strain(s): 39
DOI: 10.1007/s10811-014-0484-2

Yoshikawa, K., Aikawa, S., Kojima, Y., Toya, Y., Furusawa, C., Kondo, A., Shimizu, H. 2015 Construction of a genome-scale metabolic model of Arthrospira platensis NIES-39 and metabolic design for cyanobacterial bioproduction. PLoS One, 10, e0144430 (article ID).
Strain(s): 39
PubMed: 26640947
DOI: 10.1371/journal.pone.0144430

Yoshikawa, T., Ikeda, Y., Sakata, T., Maeda, H. 2011 Cloning and analysis of the ggpS gene from cyanobacteria Arthrospira spp. involved in the synthesis of an osmolyte glucosylglycerol. Biocontrol Science, 16, 55-61.
Keywords: Arthrospira; ggpS; Glucosylglycerol; Osmolyte; Synechococcus
Strain(s): 39
PubMed: 21719990
DOI: 10.4265/bio.16.55

Tanioka, Y., Miyamoto, E., Yabuta, Y., Ohnishi, K., Fujita, T., Yamaji, R., Misono, H., Shigeoka, S., Nakano, Y., Inui, H., Watanabe, F. 2010 Methyladeninylcobamide functions as the cofactor of methionine synthase in a cyanobacterium, Spirulina platensis NIES-39. FEBS Lett., 584, 3223-3226.
Keywords: adeninylcobamide; cobalamin; cyanobacteria; methionine synthase; pseudovitamine B12; Spirulina platensis
Strain(s): 39
PubMed: 20558164
DOI: 10.1016/j.febslet.2010.06.013

Yukino, T., Hayashi, M., Inoue, Y., Imamura, J., Nagano, N., Murata, H. 2005 Preparation of docosahexaenoic acid fortified Spirulina platensis and its lipid and fatty acid compositions. Nippon Suisan Gakkaishi, 71, 74-79 (in Japanese with English summary).
Keywords: docosahexaenoic acid; DHA; Spirulina; foods with function claims
Strain(s): 39
DOI: 10.2331/suisan.71.74

Toyomizu, M., Suzuki, K., Kawata, Y., Kojima, H., Akiba, Y. 2001 Effective transformation of the cyanobacterium Spirulina platensis using electroporation. J. Appl. Phycol., 13, 209-214.
Keywords: Arthrospira platensis; cyanobacterium; blue-green alga; electroporation; feed enzyme; poultry; Spirulina; transformation
Strain(s): 39
DOI: 10.1023/A:1011182613761

Watanabe, F., Miyamoto, E., Nakano, Y. 2001 Inactive corrinoid-compound significantly decreases in Spirulina platensis grown in a cobalt-deficient medium. J. Agric. Food Chem., 49, 5685-5688.
Keywords: Vitamin B12; pseudovitamin B12; algal health food; spirulina tablet; intrinsic facto
Strain(s): 39
PubMed: 11714378
DOI: 10.1021/jf010733i

Suzuki, S., Tazawa, R., Miya, A., Adachi, T., Kanki, R., Toyobe, M., Oguchi, M. 1994 Gas revitalization by microalgae. I. Studies on species and culture condition. CELSS J., 7, 23-28 (in Japanese with English summary).
Keywords: microalgae; Spirulina; SOT medium; culture condition; CO²/O² gas exchange system
Strain(s): 39, 45

Watanabe, M. M., Kasai, F., Hiwatari, T., Suda, S., Nei, T. 1984 Cryopreservation of microalgae in liquid nitrogen - Survival of algal cultures after freezing. Jpn. J. Freez. Dry., 30, 23-26 (in Japanese with English title).
Strain(s): 18, 29, 39, 45, 74, 75, 77, 95, 100, 120, 131, 135, 379

Watanabe, M. M. 1977 Biosystematics in Closterium of sexual unicellular green algae and Calothrix and Spirulina of asexual filamentous blue-green algae, with special reference to the analyses of natural populations. Thesis D. Sci., Hokkaido University, 114 pp., 28 tables, 54 figs.
Strain(s): 39, 45, 51, 56, 66, 68

Watanabe, M. M. & Ichimura, T. 1977 Fresh- and salt-water forms of Spirulina platensis in axenic cultures. Bull. Jpn. Soc. Phycol., 25, Suppl. (Mem. Iss. Yamada), 371-377.
Strain(s): 39, 45

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