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Strain number NIES-257  
Phylum Chlorophyta  
Class Chlorophyceae  
Scientific name Hafniomonas laevis Nakada, Suda & Nozaki  
Former name Hafniomonas montana (Geitler) Ettl et Moestrup  
Common name Green alga  
Locality (Date of collection) inside NIES Onogawa, Tsukuba Ibaraki Japan (1983-10-30)  
Latitude / Longitude 36.048471 / 140.117609 
Habitat (Isolation source) Freshwater (Pool water)  
History < Suda, Shoichiro  
Isolator (Date of isolation) Suda, Shoichiro (1983-10-30)  
Identified by Inouye, Isao; Nakada, et al. (Reidentify)  
State of strain Subculture; Unialgal; Clonal; Axenic[2014 Jan]  
Culture condition
(Preculture condition)
Medium:  C  
Temperature:  20 (20) C
Light intensity:  10-17 (10-17) µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  1 M (14 D)  
Gene information Plastid DNA ( KT625415 ) , 18S rRNA ( AB101517 ) , atpB ( AB101504 ) , psaB ( AB101515 ) , rbcL ( AB101509 AB101510 )  
Cell size (min - max) 15 - 17 μm  
Organization Unicellular; Flagellate 
Characteristics Genome decoded strain (Lemieux, C. et al. 2015)  
Other strain no. Other strain no. : OUT-5  
Lemieux, C., Vincent, A. T., Labarre, A., Otis, C., Turmel M. 2015 Chloroplast phylogenomic analysis of chlorophyte green algae identifies a novel lineage sister to the Sphaeropleales (Chlorophyceae). BMC Evol. Biol., 15, 264 (article ID).
Keywords: Chlorophyceae; Chlamydomonadales; Golenkiniaceae; Treubarinia, Jenufa genus; Plastid genome; Flagellar apparatus
Strain(s): 257425 
DOI: 10.1186/s12862-015-0544-5

Nakada, T., Suda, S., Nozaki, H. 2007 A taxonomic study of Hafniomonas (Chlorophyceae) based on a comparative examination of cultured material. J. Phycol., 43, 397-411.
Keywords: Chlorophyceae; Hafniomonas; morphology; new species; taxonomy
Strain(s): 257656171417151716171717181720 

Nakazawa, A., Nozaki, H. 2004 Phylogenetic analysis of the Tetrasporalean genus Asterococcus (Chlorophyceae) based on 18S ribosomal RNA gene sequences. J. Jpn. Bot., 79, 255-261.
Keywords: 18S rRNA gene; Asterococcus; Palmellopsidaceae; phylogeny; Tetrasporales
Strain(s): 257656 

Nozaki, H., Misumi, O., Kuroiwa, T. 2003 Phylogeny of the quadriflagellate Volvocales (Chlorophyceae) based on chloroplast multigene sequences. Mol. Phylogenet. Evol., 29, 58-66.
Strain(s): 257656 

Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55.
Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection
Strain(s): 19212223242526272829303132333435363738394041444546505152535455565758596061626364656667686970737475767778798081878990919293949596979899100101102103104105106107108109110111112119120122123124125127128129130131132133134135137138139144147148150151152153154155156157158159160162163164165166167168169170171172173174175176177178179180181182183185186187188189191192193194195197198199200201202203204205206207208209210211212213214215216217224226227228229230231232241242243244245246248257259261262263266267268271287288289290294295297298299300301302303305306308309310311312313329334336337338339340341342349351359360361362375378379380382384385390394396397398415416418419421422423424425426427428429430431432433434436437438439440446447448449450451452453454455456457458459460464468469474478479480481503504505506507509510512514515522523524527528529530531532536537538539540541542543544545546564565566567568569570571572573574575576577578579580581582583584585592593594595596597598604610628630631632633634635636637638639640641642643644645646647648649650651652653654655656657658659660661662663664665666667672685686687688689690691692693694717718719720721722723724725726727728729 

Suda, S., Watanabe, M. M. 1995 Notes on microalgae in Japan (2). Hafniomonas montana (Geitler) Ettl et Moestrup (Chlorophyceae, Dunaliellales). Jpn. J. Phycol. (Sôrui), 43, 40-41 (in Japanese with English title).
Strain(s): 257 

Watanabe, M. M., Kasai, F., Hiwatari, T., Suda, S., Nei, T. 1984 Cryopreservation of microalgae in liquid nitrogen - Survival of algal cultures after freezing. Jpn. J. Freez. Dry., 30, 23-26 (in Japanese with English title).
Strain(s): 1819212426293139404144454650737475767780818788899091939598991001011201221311351371441512042072132572633794152219223522392579 

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